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Biomedical subjects

A A Abramov

Publications and source records attributed to A A Abramov.

10 recordsLinked to original sources
PubMed2003

[The methodological specificity of PCR-test of gene p53 in plasma and blood cell DNA of oncological patients].

The evaluation of mutations in the p53 gene is of a big value for the laboratory diagnostics in oncology. The p53 gene was analyzed, within the present case study, in blood plasma, in which tumor-origin DNAs were detected, as well as in blood cells and in tumor tissue. Optimal conditions were defined for polymerase chain reaction (PCR) with primers. The adequacy of the conditions selected for PCR and for SSCP-electrophoresis, when used in the analysis of mutations in exons 5-8, gene p53, was shown by the method of direct sequencing. The first results on revealing the frequency of gene-p53 mutations were obtained for some of the oncology diseases in the studied population.

Blood Cells↗
PubMed1992

[The effect of verapamil on the course of stenocardia of effort, on the blood serum lipid level and on the basic indices of carbohydrate metabolism during the long-term use of the preparation].

The therapeutical efficiency of verapamil given for 3 months was studied in 36 patients with stable exercise-induced angina by multistep gradually increasing bicycle ergometry exercise test. The effects of the agent on serum lipid levels were evaluated in 30 cases. The therapeutical effect was achieved in 24 patients; verapamil caused a pronounced increase in physical working capacity just during the acute drug test in all cases and showing growing effects within a month of treatment. No increase in therapeutical effects was observed in 12 cases in whom a rise in exercise time was insignificant during the acute drug test. Verapamil produced no noticeable effect on fasting serum glucose and insulin levels after glucose tolerance test in 19 healthy individuals and 11 patients with lower carbohydrate tolerance. Thirteen patients with primary hypercholesterolemia had decreased levels of total cholesterol due to low density lipoprotein cholesterol.

Adult↗
PubMed1991

[Hemostasis and the blood lipid spectrum in children with a hereditary disposition for arterial hypertension and the autonomic vascular dystonia syndrome].

Overall 75 children aged 7-13 years with hereditary load as regards arterial hypertension were examined. Hemocoagulation, the content of total cholesterol and triglycerides in blood serum, and that of cholesterol in high density lipoproteins were estimated in children with and without vegetovascular dystonia. The changes in blood lipids of the atherogenic nature, activation of the processes of coagulation were coupled with vegetovascular dystonia and showed up maximally when combined with initial sympathicotonia, hypersympathicotonic reactivity, and with the hyperdiastolic variety of the clino-orthostatic test. The children with hereditary load as regards arterial hypertension and with vegetovascular dystonia should be attributed to the high risk group in order to carry out follow-up studies and a complex of treatment and health measures.

Adolescent↗
PubMed1980

[Extinction coefficient of thyroglobulin].

The extinction coefficient at 280 nm of the solution (pH 7,2-7,4) of thyroglobulin from normal thyroid and nodular euthyroid goiter was measured with the values of 0,6-0,7 to 0,9 ml.mg-1.cm-1. The value of E1%280 as function of the iodoamino acid content depends on the morphological state of the thyroid gland. A simple method for determination of the E1%280 value by measuring of the protein absorption spectrum is proposed.

Amino Acids↗
PubMedDate not supplied

[Preeclampsia of pregnant women complicated by HELLP syndrome].

One case of a Hell syndrome in a female of 22 with gestational eclampsism is described. The syndrome seemed to develop 2 weeks before hospitalization and manifested with brain hemorrhage followed by obstruction of liquor spaces. Morphologically, HELL syndrome was characterized by multilobular liver necrosis, hemoglobinurea nephrosis and pronounced hemorrhagic diathesis.

Abortion, Therapeutic↗
PubMedDate not supplied

[Characteristics of methyl-specific PCR-test of glutathione-S-transferase P1 gene in plasm DNA and cellular urinary precipitate for differential diagnosis of prostatic adenoma and adenocarcinoma].

The study was made of possibilities of methyl-specific PCR-test of glutathione-S-transferase P1 (GSTP1) gene in differential diagnosis of prostatic cancer: sensitivity of the test, comparison of reagents of Russian and foreign production. Blood plasm and cellur urinary precipitate DNA was investigated in patients with prostatic adenocarcinoma. The results show that the above test with domestic reagents is rather perspective for differential diagnosis.

Adenocarcinoma↗
PubMedDate not supplied

[Differences in the secondary structure of thyroglobulin under normal conditions and in nodular goiter].

Intact and iodine-treated thyroglobulin from normal thyroid gland and from nodular euthyroid goiter were studied by means of circular dichroism to detect differences in the protein secondary structure. Contents of alpha- and beta-structures in thyroglobulins were differentiated only by several per cents but artificial iodination of the protein increased markedly the difference between thyroglobulins of various origin. Extrapolation of the data to "zero" content of iodine-containing amino acids demonstrated that the initial steps of thyroglobulin synthesis occurring before iodination "in vitro", appears to be also responsible for alterations in the protein secondary structure.

Circular Dichroism↗