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Biomedical subjects

A A Andreev

Publications and source records attributed to A A Andreev.

17 recordsLinked to original sources
PubMed1988

[Use of enzyme-antibody immune complexes in serological analysis].

In model experiments with simulation of rabbit immune response to mouse IgG and antibody production in echinococcosis patients the indirect enzyme-linked immunosorbent assay has been several times more sensitive with peroxidase-antiperoxidase and catalase-anticatalase complexes than with enzyme-antiglobulin chemical conjugates. The immune complexes have been found to retain their activity for 6 months and longer.

Animals↗
PubMed1986

Depression of neuron responses to acetylcholine by combined application of norepinephrine and substrates of the tricarboxylic acid cycle.

The possible relationship between the function of nicotinic acetylcholine receptors in Lymnaea stagnalis neurons and energy metabolism was studied. Oxidative phosphorylation was activated by treatment of neurons with substrates of the tricarboxylic acid cycle and norepinephrine. Transmembrane currents induced by acetylcholine in isolated neurons were measured by voltage clamp. Succinate dehydrogenase activity was determined histochemically in the same neurons. Cyclic adenosine monophosphate concentration in ganglia were assayed by the protein saturation method of Gilman (1970). When used alone, succinate depressed the responses of about 50% of neurons to acetylcholine. Norepinephrine did not affect the acetylcholine-induced currents but almost doubled the inhibitory action of succinate. The mixture of norepinephrine and isocitrate also diminished the responses to acetylcholine but to a lesser extent than norepinephrine with succinate. A short-term exposure of the ganglia to succinate with norepinephrine led to the activation of succinate dehydrogenase in neurons and a threefold increase in cyclic adenosine monophosphate concentrations in ganglia. When used alone, norepinephrine doubled the cyclic adenosine monophosphate concentration. The results obtained suggest energy-dependent regulation of acetylcholine receptors.

Acetylcholine↗
PubMedDate not supplied

[Effect of prostaglandin E2 and its derivatives on the reaction of isolated mollusk neurons to acetylcholine].

A study was made of the effect of PGE2, ethyl ether and a carbohydrate derivative of PGE2 on the sensitivity to acetylcholine (ACh) of identified neurons of the mollusk pond snail. The response recorded in the mode of membrane potential fixation diminished in the presence of PGE2 and its derivatives. The rate of the decrease and the degree of the response blockade depended on the substance concentration. Washing of PGE2 made the neuronal sensitivity to ACh return to normal. With the use of PGE2 derivatives the sensitivity recovery was incomplete. The radioimmunochemical technique has demonstrated PGE2 to raise the cAMP level in the pond snail brain.

Acetylcholine↗