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A A Bondar'

Publications and source records attributed to A A Bondar'.

12 recordsLinked to original sources
PubMed1988

[Construction of probes for hybridization with hepatitis A virus RNA based on phage M13].

Three fragments of a DNA copy complementary to tre hepatitis A virus RNA have been isolated from the pHAV-VPI-22 plasmid and cloned in M 13 mp 8 vector. The 140, 250 and 390 b. p. fragments corresponded to the viral genome fragment coding for VP1 protein. Single-stranded DNAs of the hybrid phages with 250 b. p. and 390 b. p. inserts have been used for radioactive probe synthesis. The specificity of the probes for viral RNA was confirmed in hybridization with hepatitis A virus RNA purified from the virus-infected cell culture. The hybridization was shown to detect up to 10(-12)-10(-13) g of the virus. A principal possibility to use the probes in diagnostic purposes was demonstrated by the virus detection in blood samples obtained from patients with hepatitis A infection.

Coliphages↗
PubMedDate not supplied

[The isolation and expression in E. coli of a recombinant plasmid containing the 3'-terminal fragment of the cDNA of the influenza A virus nucleoprotein gene].

A recombinant plasmid pN62 determining the synthesis of a hybrid protein consisting of a full-size beta-galactosidase and C-terminus fragment of influenza A virus nucleoprotein was constructed. The complete identity of pN62 insert with the 3'-terminus cDNA fragment of influenza A virus NP-gene and conservation of beta-galactosidase reading frame was confirmed by Maxam-Gilbert sequencing of pN62. An expression of pN62 plasmid in E. coli JM103 in the presence of IPTG resulted in accumulation of fused protein as poorly soluble inclusion bodies in the bacterial cells. Analysis of E. coli JM103/pN62 bacteria lysates by 7% PAGE revealed that molecular weight of hybrid polypeptide was 18 kDa heavier than normal beta-galactosidase and corresponded to the previously deduced weight of 135 kDa.

Base Sequence↗