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Biomedical subjects

A A Budnitskiĭ

Publications and source records attributed to A A Budnitskiĭ.

4 recordsLinked to original sources
PubMed1981

[Growth of continuous and primary cell cultures on a liquid surface].

A possibility to culture reinoculated BHK-21 cells and primary human embryo fibroblasts on the surface of liquid fluorocarbon is shown. Two types of growth of BHK cells on liquid surface can be distinguished: with the spreading of cells on the surface and formation of a monolayer, and with growth without cell spreading and with accumulation of cells in the form of clusters and small groups. Adhesion of cells to the liquid fluorocarbon surface is found to be weaker compared to the reproduction on the solid surface.

Cell Adhesion↗
PubMedDate not supplied

[Location of adenine nucleotides in striated fibers of skeletal muscles].

The absorption of light at 265 nm wave length in isolated muscle fibers and myofibrilles of vertebrate striated muscle was investigated with the aid of microscope with the wide spectral range (240--900 nm), equipped with image intensifier and recording cine camera. It was established that freshly isolated nonfatigue frog fibers absorbed UV-light along the entire of the length of the fiber. This fact attests the uniform distribution of ATP and some other nucleotides in the myoplasma of muscle cell. The fatigue of the fibers results in the appearance of a cross--striated pattern along the length of fiber, determinated by the alternation of the sarcomere regions with different degree of light absorption at the given wave length. The regions of the most heavily absorption of UV corresponds to the I-, M-, and N-bands of sarcomere. The comparison of the data obtained from intact and glycerinated fibers and also from myofibrilles with extracted A-bands allows to suppose that the absorption bands at 265 nm wave length are the places of location of bound ADP, RNA and minor fraction of ATP.

Adenine Nucleotides↗
PubMedDate not supplied

[Conformational fluctuations in chloroplasts].

Characteristic features of conformational changes in chloroplasts have been studied by microfilm technique at BMI-13 installation. The oscillatory character of changes in chloroplast cross-section area in time, determined earlier by the tele-lazer microscopy technique, has been confirmed by our data. When chloroplasts are simultaneously seen in the microscope it is possible to observe their synchronous oscillation. The stationarity check of conformational changes shows that is a non-stationary process in the general case. So it is concluded that the standard technique of analysing accidental non-stationary processes can be used for study of conformational changes in chloroplasts provided that the corresponding parts of their kinetics are selected or trends are eliminated depending on the type of non-stationarity.

Biometry↗