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Biomedical subjects

A A Del'vig

Publications and source records attributed to A A Del'vig.

At least 19 recordsLinked to original sources
PubMed1995

[Collagen metabolism in hypertrophic and keloid scars].

Imbalance between the synthesis and degradation of collagen is a common sign of tissues of hypertrophic and cheloid scars. The fact that the collagen of abnormal scars of the both types have a polyfunctional linkage with a structure of pyridinol and high amounts of a polyfunctional linkage with a structure of pyrrole (Ehlich's chromogen) in the collagen of cheloid scars support the assumption that the collagen synthesis-degradation imbalance may be due to the enhanced resistance of structurally unchanged collagen to collagenolytic enzymes. The comparative studies of the ratio of type 1 collagen homotrimers to heterotrimers, that of type 1 collagen dimers to monomers, and that of types 1 to type 3 collagen in the pepsin extracts of abnormal scars of various age and patients' intact skin suggest that there are certain differences in the magnitude and scar age-related changes in the expression of collagen genes and posttranslational collagen modifications in the tissue of hypertrophic and cheloid scars. It is suggested that age-specific time course of collagen metabolic and structural changes in the abnormal scars may result from their alterations of the population composition of fibroblasts with a different phenotype.

Cicatrix, Hypertrophic↗
PubMed1991

[Age changes in the level of pyridinoline cross-linking and ratio of soluble and insoluble collagen in human rib collagen].

The changes in the content of mature crosslinks with pyridinoline structure and soluble/insoluble collagen ratio in the costal cartilage tissue of human beings aged from 1 month to 57 years were found to be age-dependent. The effect of the pyridinoline crosslink content on the soluble/insoluble collagen ratio in human costal cartilage tissue may constitute no less than 67% of the total influence of the sum of all factors. The pronounced nonlinearity of the studied dependencies points to a possible involvement of a factor(s) other than the pyridinoline crosslink content.

Adult↗
PubMed1990

[The protective activity of polyclonal and monoclonal antibodies to the lipopolysaccharide of Neisseria meningitidis serogroup A in in vivo experiments].

The protective activity of the sera of mice immunized with the preparations of native and detoxified N. meningitidis lipopolysaccharide (LPS), group A, as well as with monoclonal antibodies to N. meningitidis antigens, groups A and B, was studied on the mucin model of meningococcal infection. The study showed that the maximum level of anti-LPS antibodies in mice was observed on day 7 after the injection of LPS. Immune sera obtained from mice were capable of protecting the animals from fetal meningococcemia induced by N. meningitidis strains of homologous and heterologous groups. As shown by the results of this study, the alkaline treatment of N. meningitidis native LPS did not decrease the protective properties of antibodies. The monoclonal antibodies under study were found to possess high preventive activity in mice challenged with N. meningitidis, groups A and B. Anti-LPS monoclonal antibodies showed greater protective activity than antipolysaccharide monoclonal antibodies.

Animals↗
PubMed1989

[The coagglutination reaction for detecting diphtheria toxin].

High-titer antidiphtheria antitoxic rabbit serum has been obtained, and on the basis of this serum a coagglutinating diagnosticum has been developed. The sensitivity of the test has been found to depend on the content of antitoxic antibodies in the serum and on its purity. Diagnostica prepared from native serum containing 500 I. U./ml (a titer of 1:51, 200 in the passive hemagglutination test) permit the detection of 0.02-0.03 Lf/ml of diphtheria toxin. A decrease in antibody titer to 5-25 I. U./ml leads to a drop in sensitivity to 0.2-2 Lf/ml. The use of LgG fraction and pure antibodies increases the sensitivity of the test to 0.002-0.003 Lf/ml. The possibility of detecting toxin in Corynebacterium diphtheriae strains is shown.

Agglutination Tests↗
PubMed1989

[The protective activity of the detoxified lipopolysaccharide of Neisseria meningitidis serogroup A in in vivo experiments].

The immunogenic potency, toxicity, homologous and heterologous protective activity of lipopolysaccharide preparations obtained from serogroup A N. meningitidis (LPS A) were studied in animal experiments. These preparations were shown to possess very high protective activity. The alkaline treatment of native LPS A decreased the toxicity of the preparation almost 20 times and did not affect its immunogenic potency. Detoxified LPS A was capable of protecting mice from fatal meningococcemia resulting from infection with N. meningitidis strains, serogroups A, B and C; the adsorption of the preparation on aluminium hydroxide did not affect its protective activity. In view of the properties of detoxified LPS A revealed in this investigation, it may be regarded as a possible vaccinal preparation.

Adsorption↗
PubMed1988

[Heterogeneity of collagen molecules types I and II according to their resistance to proteolysis].

Study of the effects of pepsin treatment on soluble collagens type I of the skin and collagens type II of the costal cartilage of healthy subjects revealed the presence of two classes of molecules differing in the stability of their three-helical structure. In collagen molecules possessing a low stability (their number may amount to 20-30%) within the temperature range of 4-30 degrees C pepsin causes a split-off of N-terminal sites with the formation of short chains, i.e., alpha 1(I), alpha 2(II), and alpha 1(II), whereas at higher temperatures (33 degrees C for collagens type I and 37 degrees C for collagens type II) a complete degradation of these molecules takes place. It was found that collagens types I and II molecules contain a high number of three-helical sites with a high susceptibility to pepsin. The putative functional role of structural heterogeneity of collagen molecules is discussed.

Cartilage↗
PubMed1988

[Kinetics of protein degradation in diploid and trisomic human fibroblasts].

The degradation rate of long-lived and short-lived proteins was determined in diploid fibroblasts and fibroblasts with trisomy 7 derived from human embryos. Two fractions of proteins were detected in the exponentially growing diploid fibroblasts with half-lives (T 1/2) 37 and 19 hours. The rate of protein degradation increases in diploid fibroblasts as they approach confluence and protein fractions with T 1/2 30, 18 and 12 hours appear. The rate of protein degradation in trisomic fibroblasts does not change for the long-lived and short-lived proteins and is the same in both exponential (T 1/2 31 and 14 hours) and stationary phase (T 1/2 33 and 17 hours). The relative amount of the short-lived proteins in trisomic fibroblasts in the stationary phase decreased as compared with the one in diploid fibroblasts. It is apparent that a mechanism of regulation of protein catabolism in trisomic fibroblasts is impaired.

Cells, Cultured↗
PubMed1987

[Cultivation of murine B-cell hybridomas in the spleen].

The tumorigenic capacity of mouse B-cell hybridomas in both cloned and primary cultures was studied. The cells were selected for inoculation from 24-well plates and introduced into the spleen of syngeneic mice. The cells took in 50% of the animals. The cells of hybridoma tumors induced as the result of intrasplenic inoculation, when subcultured in the second passage following the standard scheme, i.e. inoculated intraperitoneally in a dose of 1 X 10(7) cells into mice previously treated with vaseline oil or pristane, produced tumors in 100% of the animals.

Animals↗
PubMed1987

[Abnormal structure of type II collagen in a patients with funnel chest].

The electrophoretical analysis and CNBr-peptide mapping of the collagens, isolated from the costal cartilage of 30 patients with non-classified and syndromal forms of pex excavatum (funnel chest) (27 patients) and pex carinatum (3 patients) was carried out. In case of one patient with the nonclassified form of funnel chest the electrophoretical mobility of CB 9.7-peptide was found to be decreased. The electrophoretical mobilities of other peptides are not markedly changed. The data obtained allow one to suggest the mutation causing the defect in the region about 160 amino acid residues distant from the C-end of alpha 1 (II) chain of type II collagen of the patient.

Adolescent↗
PubMed1987

[Characteristics of intracellular metabolism of procollagen and other proteins in human embryo fibroblasts in trisomy for chromosome 7].

A comparative study of the relative rates of intracellular total protein metabolism in diploid and aneuploid (with trisomy for chromosome 7) human embryo fibroblasts in the logarithmic and stationary growth phases was carried out. Using double labeling with [14C]proline (24 hrs) and [3H]proline (3 hrs), it was found that: the rates of intracellular protein metabolism during transition to the stationary phase of growth are increased in diploid cells and decreased in cells with trisomy for chromosome 7; the relative rate of protein metabolism in the logarithmic phase is higher in trisomic cells than in diploid ones. The intracellular degradation of procollagen in trisomic cells is increased approximately by 17% as compared to normal fibroblasts. Treatment of cell lysates with bacterial collagenase revealed the presence of procollagen incomplete degradation products in anomalous fibroblasts. The observed differences in the rates and mode of protein metabolism during transition of diploid and trisomic fibroblasts to the stationary phase of growth suggest that the odd autosome interferes with the normal coordinated activity of genes in chromosomes.

Chromosomes, Human, Pair 7↗
PubMed1985

[Comparative characteristics of protein-synthesizing apparatus of diploid and aneuploid human embryo fibroblasts].

The time of average polypeptide chain synthesis (tc), distribution of synthesized polypeptides according to their molecular masses, the ratio of translating and nontranslating ribosomes and polyribosomes of different size have been analyzed for diploid and aneuploid strains of fibroblasts. The magnitude of tc as well as the size of polypeptide chains synthesized were found to be similar for both kinds of fibroblasts. The relative cellular content of the translating ribosomes has been shown to decrease during the transition of cells from both strains to the stationary growth phase. The relative content of heavy polyribosomes is lower in aneuploid cells as compared with that in diploid cells. The process of translation in aneuploid fibroblasts is concluded to have no essential deviations from normal.

Aneuploidy↗
PubMed1981

[Interrelation between protein synthesis and nucleo-cytoplasmic transport of messenger RNA in cells of rat livers in different stages of regeneration].

Synthesis of nonribosomal RNA was increased 2-2.5-fold in nuclei of rat liver cells at early steps of regeneration /2 days after partial hepatectomy/under conditions of protein synthesis inhibition by means of cycloheximide within 3 hrs. Amount of mRNA, transferred from nuclei into cytoplasm, was also 2-fold higher under these conditions as compared with control cells. The mRNA, transferred from nuclei into cytoplasm under the conditions of cycloheximide treatment of liver cells at early steps of regeneration, did penetrate the polyribosomes although the protein synthesis did not occur on the polyribosomes formed. At the later steps of regeneration /within 5 days after partial hepatectomy/ under the conditions of protein synthesis inhibition by cycloheximide within 3 hrs, formation of nonribosomal RNA was decreased by 40% in liver cell nuclei; amount of mRNA, transferred into cytoplasm, was also decreased by 40%. When the cycloheximide treatment was carried out during 1 hr, synthesis of nonribosomal RNA was increased 1.5-fold in liver cell nuclei at the later steps of regeneration although the amount of mRNA, transferred from nuclei into cytoplasm, was decreased by 30% as compared with the control cells. The data obtained suggest that during rat liver tissue regeneration a change occurred in the molecular mechanisms linking translation, biosynthesis and nuclear-cytoplasmic transport of mRNA of "tumoral" type/at early steps of regeneration/ or "normal type"/ at later steps of regeneration.

Animals↗