PubMed Health⌕ Search

Biomedical subjects

A A Iakovlev

Publications and source records attributed to A A Iakovlev.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

[Radioimmunologic determination of aldosterone in the peripheral blood of diabetic patients].

The paper treats of the specific, accurate, highly sensitive, economic radioimmunological method of aldosterone determination. The authors used commercial reagent kits for the radioimmunological determination of aldosterone, this facilitating the work of investigators, and permitting to examine many samples for a short period of time. The method can be suggested for both the experimental and clinical practice.

Adult↗
PubMedDate not supplied

[Pentylenetetrazole kindling induces caspase-3 activation in rat brain].

Pentylenetetrazole kindling (but not a single pentylenetetrazole injection) induced caspase-3 activation in the cerebral cortex, hippocampus, and cerebellum of rats as well as neurodegeneration in the hippocampus. The number of neurons in the CA3 subfield of the hippocampus decreased significantly, whereas no apoptotic nuclei could be detected. The results support a possible non-apoptotic involvement of caspase-3 in brain plasticity.

Animals↗
PubMedDate not supplied

[Transcutaneous electrostimulation of the primary elements of the visual system in children after the extraction of a congenital cataract].

Courses of transcutaneous electrostimulation of the visual analyzer periphery according to E. B. Kompaneets were administered to 31 children (56 eyes) aged 4 to 12 because of low vision acuity after congenital cataract extraction. The amplitude of stimulating pulses was from 150 to 400 microA. The first course consisted of 5-8 sessions, repeated courses of 4 sessions. The results were assessed by vision acuity check-ups and recordings of visual [correction of auditory] evoked potentials (AEP). Vision acuity improved from 0.1 to 0.9 after a course of treatment. AEP amplitude was reduced in all the patients to 15.4 microV on an average, the time of the pulse conduction in the auditory system was normal (113.2 ms). Electrostimulation effects on the AEP were negligible. Electrostimulation may be recommended to children with low vision acuity and low values of the AEP amplitude after congenital cataract extraction.

Cataract↗
PubMedDate not supplied

[Morbidity: terminology, mechanisms of formation and classification].

In the 70-ies of the last century the evolution of the epidemic process doctrine resulted in developing population approach to morbidity analysis, in addition to the traditional focal approach. On the one hand, this led to the discussion on the possibility of applying such notion to non-infectious diseases and, on the other hand, to the discussion on the mechanisms of the development and formation of different manifestations in infectious diseases. In both cases the discussion was centered on the phenomenon of morbidity. The present article deals with the nature of morbidity as a phenomenon and the acceptability of general scientific approaches in the interpretation of seasonal and cyclic character of morbidity from the viewpoint of the wave process in an open system. On the basis of the data presented in this work the terms used in epidemiology in considering manifestations of the epidemic process are discussed. The improved classification of morbidity by qualitative and quantitative signs for infectious diseases is proposed.

Bacteria↗
PubMedDate not supplied

[Caspase-3 inhibition blocks long-term potentiation in hippocampal slices].

Incubation of hippocampal slices with Z-DEVD-FMK, a specific inhibitor of caspase-3, elicits a time dependent decrease in long-term potentiation (LTP). After 4 hours or later after the incubation with Z-DEVD-FMK the tetanization fails to induce LTP. However, Z-DEVD-FMK does not affect basal indices of synaptic plasticity and short-term plasticity (population spike amplitudes and paired pulse facilitation). The results are the first evidence for the involvement of caspase-3-mediated mechanisms in long-term potentiation phenomenon.

Animals↗
PubMedDate not supplied

[Using thioGlo-1 for the determination of thiols in brain tissue].

A technique for the fluorometric determination of protein and non-protein thiols in brain tissue with the maleimide reagent ThioGlo-1is has been described. Comparison with the most popular spectrophotometric Ellman method has been performed. The ThioGlo-1-based method is highly sensitive and may be used for thiol determination both in homogenates and supernatants of brain tissue.

Animals↗
PubMedDate not supplied

[Effects of morphine withdrawal on the indices of free radical homeostasis and nitric oxide system in rat liver and thymus].

Indices of oxidative stress, nitric oxide (NO) metabolism as well as the activity of caspase-3, an important enzyme of apoptotic cell death, were measured during the morphine withdrawal syndrome in liver and thymus of rats. Male Wistar rats were administered with morphine hydrochloride (i.p., at increasing doses from 10 to 100 mg/kg, twice a day, for 6 days). Thirty-six hours after the last administration the withdrawal syndrome was monitored using the specific autonomic and locomotor indices. During this period, weights of body and thymus significantly decreased. Oxidative stress in liver was accompanied by an increase in aspartate aminotransferase and gamma-glutamyl transferase in blood serum. No signs of oxidative stress could be demonstrated in thymus. The activity of the Ca(2+)-dependent isoform of nitric oxide synthase (NOS) in liver increased, while, the activity of the Ca(2+)-independent NOS diminished, the total activity of NOS in liver and thymus remained unchanged. The concentration of nitrates/nitrites in blood was decreased, in thymus increased, and in liver unchanged. Caspase-3 activity changed neither in liver, nor in thymus. The results are discussed from the perspective of possible antioxidant and antiapoptotic role of NO during morphine withdrawal syndrome.

Animals↗