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Biomedical subjects

A A Kruglova

Publications and source records attributed to A A Kruglova.

8 recordsLinked to original sources
PubMed1986

[New R-plasmids in strains of Serratia marcescens with multiple drug resistance].

In 4 S. marcescens polyresistant strains isolated from patients conjugative plasmids transferred to Escherichia coli have been detected. Two of these strains carry each one plasmid which codes resistance to 10 different antibiotics, including aminoglycosides which rarely occur in our country, and belongs to group IncC. The third strain is the host of 2 plasmids. One of them is similar to the above-mentioned 2 plasmids with respect to the incompatibility group and a set of markers, but additionally codes resistance to cephalosporins; the second plasmid has been determined as belonging to group IncM, unstable and capable of rendering the cells highly resistant only to aminoglycosides. And, finally, the fourth strain also carries 2 plasmids: one of them is unstable and belongs, supposedly, to group IncI alpha, and the second plasmid is stable and belongs to group IncM. The plasmid of group IncI alpha differs from all other plasmids of our Serratia by its capacity of rendering the cells highly resistant to chloramphenicol.

Anti-Bacterial Agents↗
PubMed1981

[Transfer to Escherichia coli of the mercury markers of new Pseudomonas aeruginosa plasmids].

A total of 5 new conjugative plasmids pLD can be transferred from one Ps. aeruginosa strain to other strains of this species, but not to E. coli. Only the markers of resistance to mercury can be transferred to E. coli by means of plasmids Inc P-1. The present work contains the data confirming that the mercury markers of 2 plasmids pLD 1017 and pLD 1051 behave similarly to transposons.

Conjugation, Genetic↗
PubMed1978

[Staphylococcal wound infection and the problems of antibacterial therapy].

An analysis of a qualitative composition of wound microflora grounded on the findings of the clinico-bacteriological examination of 315 cases with various pyo-inflammatory processes is presented here, and specific features of the species composition of wound microflora depending upon the character of a pyo-inflammatory process are shown. The sensitivity of staphylococcuses isolated from the wounds, to 19 antibacterial preparations is analyzed and recommendations on the most rational and differentiated use of antibacterial drugs in various forms of a purulent process of staphylococcal eti-logy are suggested.

Anti-Bacterial Agents↗
PubMedDate not supplied

[Visible and "cryptic" segregation of parental chromosomes in embryonic stem hybrid cells].

Chromosome segregation of the parental chromosomes was studied in 20 interspecific hybrid clones obtained by fusion of Mus musculus embryonic stem cells with Mus caroli splenocytes. FISH analysis with labeled species specific probes and microsatellite markers was used for identification of the parental chromosomes. Cytogenetic analysis has shown significant intra- and interclonal variability in chromosome numbers and ratios of the parental chromosomes in the hybrid cells: six clones contained all M. caroli chromosomes, nine clones showed moderate segregation of M. caroli chromosomes (from 1 to 7), and five clones showed extensive loss of M. caroli chromosomes (from 12 to complete loss of all M. caroli autosomes). Both methods demonstrated "cryptic" segregation of the somatic partner chromosomes. For instance, five clones with near-tetraploid chromosome sets contained only few M. caroli chromosomes (from 1 to 8). The data obtained suggest that the tetraploid chromosome set per se is not a sufficient criterion for conclusion on the absence of chromosome loss in the hybrid cells. Note that "cryptic" chromosome segregation occurred at a high frequency in the examined hybrid clones. Thus, "cryptic" segregation should be borne in mind for assessing pluripotency and genome reprogramming of embryonic stem hybrid cells.

Animals↗