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Biomedical subjects

A A Kuznetsov

Publications and source records attributed to A A Kuznetsov.

At least 55 records · Page 3Linked to original sources
PubMed1980

[Heterogeneity of pituitary gonadotropin from sturgeons].

A highly purified preparation of the pituitary gonadotropin of a chondrostean fish, sevryuga (Acipenser stellatus Pall.) has been shown to be homogeneous by criteria of molecular weight, sedimentation coefficient and by N-terminal amino acid analysis. On the contrary, polyacrylamide gel electrophoresis and isoelectric fucusing have revealed a marked heterogeneity of the hormone preparation. The determination of gonadotropic activity (in vitro amphibian oocyte maturation test) has shown the similar specific activities of the differently charged hormone molecules.

Animals↗
PubMed1978

[Extraction of acid alpha-glucosidase from human spleen].

An efficient method for isolation of acid alpha-glucosidase from human spleen is developed. The method involves chromatography of the enzyme on rho-aminophenyl-alpha-D-glucopyranoside covalently bound to CH-Sepharose 4B, with subsequent gel filtration on Sephadex G-200. The enzyme was homogeneous by the polyacrylamide gel electrophoresis data; it was purified about 1500-fold, as compared with the crude extract (the total yield 12.5%). Besides acid alpha-glucosidase, the preparations of alpha-L-fucosidase, alpha-D-galactosidase and beta-N-acetylglucosaminidase were isolated and purified 200-, 130- and 280-fold, respectively. The nature of interaction between acid alpha-glucosidase and immobilized rho-aminophenyl-alpha-glucopyranoside is discussed.

Chromatography, Gel↗
PubMed1978

[Sutureless anastomoses in gastrointestinal surgery with and without steady magnetic field (experimental study)].

Experimentally in 52 dogs, sutureless gastro-duodenal and ceco-jejunal side-to-side anastomoses were done by means of devices proposed by N.N. Kanshin for connecting void organs, and the features of accretion of the organ walls under the effect of both simple mechanical compression and that with the help of a steady magnetic field were studied. In both variants the process of anastomosis formation completes at 6-7 days after the operation and natural elimination of the compressing elements occurs at 7-8 days. Morphological examinations of sutureless anastomoses formed under a magnetic field or without it revealed no differences between them. The experimental results demonstrated a number of significant advantages of the sutureless methods of tissue connection over the traditional manual intestinal suture.

Animals↗
PubMedDate not supplied

[Distribution of beryllium in the organs of rats].

Tests with albino female-rats evidenced that following intravenous, intraperitoneal and intramuscular administration of Be to critical organs the ones most likely to be implicated proved the liver, kidneys and the skeleton, while with intratracheal introduction it were the lungs.

Animals↗