A A Olthof | PubMed Health

PubMed1988

Osteoblastic differentiation.

The fully differentiated osteoblast may be easily recognized in bone tissue. Its cuboidal shape, its position directly opposed to the bone surface and its capacity to produce calcified bone matrix are characteristic. Three other differentiation stages are also reasonably well defined--the preosteoblast, the osteocyte and the lining cell. These differentiation stages are preceded by an unknown number of precursor, progenitor and stem cell stages. Little is known about the regulation of the transitions between the various osteogenic phenotypes and their reversibility or irreversibility. One of the reasons for this is the lack of adequate tools with which to recognize the various differentiation stages. We have developed a number of monoclonal antibodies (in bone) specifically directed against osteocytes, osteoblasts and as yet unidentified cells in the periosteum. The anti-osteocyte monoclonals were used to recognize osteocytes in bone cell cultures and we obtained purified osteocyte populations for metabolic studies. Osteocytes were shown to have binding sites for parathyroid hormone. The antibodies directed against osteoblasts showed that at present our culture conditions are inadequate to allow osteoblast differentiation in vitro.

Animals↗

PubMed1988

Induction of ornithine decarboxylase activity in isolated chicken osteoblasts by parathyroid hormone: the role of cAMP and calcium.

We investigated the role of cAMP and Ca2+ as mediators in parathyroid hormone (PTH)-induced ornithine decarboxylase (ODC) activity in primary cultures of chicken osteoblasts. We present evidence that the induction of ODC activity by PTH is most likely a receptor-mediated process and that cAMP is a mediator. However, using three different approaches we have strong indications that cAMP is not the exclusive mediator of PTH-induced ODC activity. First, when the dose-response curve of PTH-induced ODC activity is compared with that of PTH-stimulated cAMP production, the ED50 for cAMP production is about five times as high as that for the induction of ODC activity. Second, 1 mM 9-(tetrahydro-2-furanyl) adenine (SQ 22.536) almost completely inhibited PTH-stimulated cAMP production whereas there was only a small inhibitory effect on PTH-induced ODC activity. Third, some PTH fragments unable to stimulate cAMP production were still able to induce ODC activity. We therefore propose that apart from cAMP, an additional messenger, most likely Ca2+, must be present. Evidence for this concept are the observations that substances affecting extracellular and intracellular Ca2+ levels (EGTA, A23187, CoCl2, verapamil) or antagonizing calmodulin (Trifluoroperazin, Compound 48/80) also strongly affect PTH-induced ODC activity. These effects could not be explained by a positive interaction of Ca2+ with the hormone-stimulated cAMP system as 2 mM EGTA strongly enhanced PTH-stimulated cAMP production but at the same time completely inhibited PTH-induced ODC activity. A similar dissociation between hormone-induced cAMP production and induction of ODC activity was found with the Ca2+ -ionophore A23187 (10(-7) M) which significantly inhibited PTH-stimulated cAMP production but strongly enhanced PTH-induced ODC activity. Our results suggest that intracellular Ca2+, and possibly calmodulin, in addition to cAMP, are involved in PTH-induced ODC activity in chicken osteoblasts. Most probably Ca2+ is the initial messenger and cAMP acts in a coordinate pattern as a synarchic messenger making the induction of ODC activity by PTH more sensitive to Ca2+. Furthermore, the present findings are in agreement with our concept of the existence of two receptors or two receptor-sites for PTH on osteoblasts. One receptor is coupled to the production of cAMP and is presumably activated when the first two aminoacids of the NH2-terminus of the hormone are present and the other, suggested to be responsible for the increase in intracellular Ca2+, is thought to be activated by a region of the hormone sequence between amino acid 3 and 34.(ABSTRACT TRUNCATED AT 250 WORDS)

1-Methyl-3-isobutylxanthine↗