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Biomedical subjects

A A Sozinov

Publications and source records attributed to A A Sozinov.

At least 19 recordsLinked to original sources
PubMed2000

[Assessment of genetic polymorphism in Colorado potato beetle Leptinotarsa decemlineata 8Say) by RAPD markers].

Four decanucleotide primers were chosen to detect polymorphism for randomly amplified polymorphic DNA (RAPD) in the populations of Colorado potato beetle. The variability of RAPD patterns was studied in three oblasts (Kiev, Moscow and Kurgan) that are geographically distant and considerably differ in ecological and climatic conditions affecting the development of this beetle: Kiev, Moscow, and Kurgan oblasts. A high level of intrapopulation polymorphism exceeding interpopulation differences was revealed. RAPD-marker subsets differing in the frequency and distribution throughout the studied populations were revealed.

Animals↗
PubMed1998

Genetics of alpha-amylases in hexaploid oat species.

The inheritance of alpha-amylases was studied in six F2 populations of hexaploid oats (Avena sativa, A. byzantina, A. fatua, A. sterillis) using polyacrylamide gel electrophoresis. A total of 22 loci was identified and described. Three main linkages of four or five loci each and an additional two pairs of linked loci were detected. It seems likely that the three main linkage groups represent homeologous chromosomes. Matching of alpha-amylase profiles of hexaploid (AACCDD), tetraploid (AACC), and diploid (AA) species was made to assign the linkage groups to particular subgenomes in the hexaploid oat. It was proposed that Linkage 1 (Amy12-Amy10-1-Amy4-Amy13-Amy11) belongs to the D-subgenome; Linkage 2 (Amy10-2, Amy9-Amy8-Amy6) belongs to the A-subgenome; and Linkage 3 (Amy7-Amy3-Amy5-Amy2) belongs to the C-subgenome of the hexaploid oat. The "malt" and "green" alpha-amylases in hexaploid and tetraploid oats have been identified. Isozymes of "green" alpha-amylase were lower in electrophoretic mobility than other isozymes and were governed by loci assigned to the A- and D-subgenomes.

Avena↗
PubMed1997

[Interlocus associations of various genetic biochemical systems in cattle].

Variation of some genetic biochemical systems (transferrin, ceruloplasmin, amylase-I, and kappa-casein) were studied in five cattle groups of different origin. Data on linkage disequilibrium demonstrated that some syntenic genes did not exhibit interlocus association. Conversely, interlocus association appeared between some loci that were located in different chromosomes. Apparently, the patterns of these associations may be specific for gene pools of individual groups from the same animal species.

Amylases↗
PubMed1988

Polymorphism and inheritance of gliadin components controlled by chromosome 6A of spring durum wheat.

The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate, pH 3.1) and two-dimensional (first dimension, Al-lactate, pH 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. Analysis of hybrids has shown that all components of the alpha zone of gliadin spectra are inherited together as blocks and are, probably, coded for by a cluster of tightly linked genes located on chromosome 6A. Fourteen variants of gliadin blocks have been identified, which can be classified into five families on the basis of component composition. All families but one have analogues among chromosome 6A-controlled blocks of bread wheat. The results indicate that some of the genome A diploid genotypes that were ancestors of durum wheats were also ancestors of bread wheats and that polyploid wheats were produced by repeated allopolyploidization events, as has been suggested earlier.

Chromosome Mapping↗
PubMedDate not supplied

[The mutational spectra of gene p53 in different types of tumors].

The comparative analysis of the frequencies of nucleotide exchanges in mutational spectra of gene p53 (5-8 exons) between germline cancer-prone families (Li-Fraumeni syndrome), between somatic mutations in the tumors of different histogenesis and cell lines, obtained from them, was carried out. The nucleotide positions with high level of mutation events (mutational "hot spots"), typical for germline mutational spectra, tissue-specific patterns of their disappearing and appearing of new ones in solid tumors in vivo, nearly complete absence of "hot spots" in lymphomas and cell lines in vitro were revealed. The obtained results allowed to suggest, that one of the leading factor controlling hot-spots distribution in 5-8 exons of gene p53 is the specificity of cell division conditions in vivo and in vitro.

Base Sequence↗
PubMedDate not supplied

[The effect of natural selection on the allelic frequency of gliadin-coding loci and their associations in an artificially created hybrid population of winter wheat].

Hybrid population with heterogeneity for five gliadin-coding loci was created by crossing of five winter common wheat cultivars (Belozerskaya 47, Ilyichevka, Kyyanka, Mironovskaya 808, Polesskaya 70) by the complete scheme. Frequencies of alleles at these loci and their associations were analyzed after two, four and ten years of resowing. The ratio of alleles at the studied loci was changed as reproduction occurred. Gli 1A5 allele displayed stable tendency to frequency increasing, Gli 1D1--a little tendency and Gli 6A3--very essential tendency. The share of Gli 6D2 allele increased only after ten years of population reproduction. Combination of alleles at two, three, and five loci was not accidental. The most common regularity was predominance of the frequency of allelic associations characteristic of the parents (except for Ilyichevka) over new combinations. Possible causes of this phenomenon are discussed.

Alleles↗
PubMedDate not supplied

[Activity of neurons in the anterolateral region of the rabbit motor cortex during taking food and nonfood objects in instrumental behavior].

We showed earlier that only 2-4% of N-neurons in the rabbit's anterolateral ("masticatory") motor cortex (AC) that are being specialized in relation to Newly formed acts (such as pressing the pedal, approaching the pedal) during the elaboration of instrumental food-acquistion behavior. The majority of neurons in this area are O-neurons that are specialized in relation to the Older acts formed long before the acquisition of instrumental behavior: mostly taking of food. It was shown also that electrical stimulation of this area produced jaw movements. It might seem reasonable to propose that the number of N-neurons may be increased in this area if pedal pressing in substituted with instrumental act like seizure and pulling a ring that is more corresponding to the "projectional property" of the AC. Here we show that the number of N-neurons in rabbit's AC remains constant -3.4%, even is the animals that were trained to pull a ring instead of pressing a pedal. Thus, we assume that the small number of N-neurons is the stable property of the AC. However, the role of the AC in the subserving of the behavior does not remain the same. Much more AC units (O-neurons) were activated in the ring pulling than in the pedal pressing instrumental act.

Animals↗
PubMedDate not supplied

[Conjugation of the 1BL/1RS translocation with qualitative and quantitative traits in the common wheat T.aestivum].

The review considers the effect of the rye 1BL/1RS translocation in the common wheat genome on qualitative and quantitative traits: grain quality, resistance to diseases, productivity and adaptivity, parthenogenesis, regeneration in anther culture, frequency of chromosome aberrations and frequency of cross-pollination. Data on special features of transmission of the 1BL/1RS translocation through male and female gametes are presented.

Crops, Agricultural↗
PubMedDate not supplied

[Recombination frequency in the locus Gli-D1 of common wheat T. aestivum L].

The recombination frequency at the gliadin locus Gli-D1 of common wheat was determined by the maximum likelihood method. Recombination was observed between the gene encoding the fastest omega-component of the allele Gli-D1j, and the genes encoding the other omega-gliadins of this allele. The frequency of recombination was 0.65 +/- 0.18% for the cross between the near-isogenic lines of winter common wheat with respect to gliadin loci Gli-D1-4 and Gli-B1-3 and 0.78 +/- 0.45% for the cross between the varieties Yunnat and B-16.

Alleles↗
PubMedDate not supplied

[Hematologic chimerism in cattle twins].

A cytogenetic examination of a cow and its offsprings, obtained by pair transplantations of the embryos was carried out. The fragile X and the heteromorphism of the chromosomes XX of this cow and some of its offsprings were revealed. The variability of the hematological chimerism of the pair transplanted embryos was observed. The immigrated cells were, as a rule, distinguished by the comparatively low frequencies of the metaphases with the nearly located chromosomes XX. The heterogeneity of the stem blood cells of the embryos for some traits, and for the ability to migrate in the blood of the twin is under discussion.

Animals↗
PubMedDate not supplied

[Comparative analysis of variability of various genetic systems in farm animals].

The particularities of the genetic structure formings of the different genetic systems (biochemical and immunogenetic markers) in the breeds, interbreed crosses of sheep and groups of cattle in connections with the locus, breed and the way of the productivity trait selections were described. The uniqueness of the characteristics and forming mechanisms of the genetic structure related with these factors and the inadequacy of the suppositions about them on the genealogical data were discussed.

Alleles↗
PubMedDate not supplied

[The heterogeneity of the cytogenetic variability in the bone marrow cells of laboratory and wild rodents in the exclusion zone of the Chernobyl Atomic Electric Power Station].

Analysis of the characters of cytogenetic variabilities and correlative interrelations between them revealed the group specificities of karyotype instability in bone marrow cells of some lines of laboratory and of wild mice under the conditions of increased radionuclide pollution in the Chernobyl zone. The complex correlative interrelations between the frequencies of cytogenetic anomalies and the character of cell division (the number of metaphases, binucleated leukocytes per 1000 cells) were observed. The obtained data indicate the stimulatory influence of chronic low doses of ionizing radiation on the manifestation of spontaneous mutagenesis, which has own genotype specific characteristics.

Animals↗
PubMedDate not supplied

[The dynamics of the allele variants of biochemical markers in generations of cattle in the exclusion zone of the Chernobyl Atomic Electric Power Station].

Analysis of the heritability of allelic variants of polymorphous genetic-biochemical systems in the cattle groups, reproduced in conditions of increased radionuclide pollution in exclusion zone of the Chernobyl AES was carried out. The disturbances of equal probability of the heritability for different allelic variants in some cattle group were revealed. It was supposed, that the appearance of the disturbances may take part in changeabilities of different gene pools under the conditions of ecological stresses. Possible mechanisms of this phenomenon were discussed.

Alleles↗
PubMedDate not supplied

[The bovine leukosis virus and the diagnosis of infected animals].

The results of selection of primers and amplification of fragments of provirus genes Gag and Env of bovine leukemia virus (BLV) in artificially infected cell line and in cattle leukocytes are presented. The distinctions between diagnoses of BLV infected animals by the testing their blood for antibodies to viral antigens and by the presence of provirus genes in DNA of their leukocytes were revealed.

Animals↗