Biomedical subjects

A A Tokmanov

Publications and source records attributed to A A Tokmanov.

3 recordsLinked to original sources

PubMed1991

[Role of active forms of oxygen in inducing luminol-dependent chemiluminescence in macrophages].

The luminol-dependent chemiluminescence of mouse peritoneal macrophages during phagocytosis of opsonized zymosan was studied by using specific active oxygen scavengers and metabolic inhibitors. Extracellular hydrogen peroxide and superoxide anion were shown to contribute immensely to the induction of the chemiluminescence. The role of the hydroxyl radical was rather insignificant, whereas singlet oxygen was not involved in this process. The interaction between luminol and peroxide was shown to be peroxidase-dependent. An inhibitory analysis revealed that the interaction between luminol, peroxide and superoxide anion obeyed a hybrid enzyme-free radical mechanism.

Animals↗

PubMed1990

[Glucose determination by using luminol].

The technique of glucose assay is based on measurements of the intensity of chemiluminescence resultant from glucose oxidation in an enzymic system containing glucose oxidase and peroxidase, as well as luminol in a saturating concentration. The sensitivity of chemiluminometric assay is by one order higher than the sensitivity of the routine colorimetric analysis. The suggested method is highly sensitive to glucose, does not involve the use of a carcinogenic stain, and may be used with a variety of the substrate concentrations: from 0.5 to 150 micrograms per sample.

Blood Glucose↗

PubMed1990

[The trigger function of cAMP in regulating the chemiluminescence of macrophages exposed to ethanol].

The capacity of mouse peritoneal macrophages to luminol-dependent zymosan-induced chemiluminescence was investigated. It was revealed that a short-term incubation (5-10 min) of macrophages in 0.05-0.50% ethanol resulted in a dose-dependent reduction of chemiluminescent response. Ethanol induce a sharp 2-3 fold increase in the intracellular cAMP content after a 2 minutes incubation. The temporal and concentration correlation of both the processes suggests that the inhibition of chemiluminescence may be mediated by the intracellular cAMP content.

Animals↗