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A A Tsutsaeva

Publications and source records attributed to A A Tsutsaeva.

At least 19 recordsLinked to original sources
PubMed1992

[The characteristics of the morphofunctional status of the adenohypophysis in lethally irradiated recipients after bone marrow transplantation].

The effect of lethal irradiation and transplantation of syngenic bone marrow in a dose of 1 x 10(7) cell/ml on the morphofunctional state of hypophysis at various stages of the posttransplantation period has been studied for 3 months using 100 linear male mice of a F1 (CBA x C57 B) line. The experiments conducted have shown that bone marrow transplantation reduces the intensity of the negative effect of irradiation on hypophysis and facilitates normalization of its histological structure. There was a correlation between changes in the number of secretory cells in the anterior lobe of the hypophysis and the level of corticosterone in irradiated and bone marrow-protected animals.

Animals↗
PubMed1991

[The effect of bone marrow transplantation on the dynamic recovery of the morphofunctional properties of the pancreas in irradiated recipients].

Effect of transplantation of syngeneic bone marrow in the dose of 1 X 10(7) cell/ml on the state of pancreatic gland in lethally irradiated recipients has been studied at different stages of posttransplantation period for 3 months using 250 linear male rats G (CBA x C57B). Histological and biochemical investigation, conducted in dynamics, have shown that transplantation of native and cryopreserved bone marrow to lethally irradiated animals facilitates activation of compensatory-restoration processes manifesting themselves in mitotic division of glandular and epithelial cells, as well as optimizes exchange of carbohydrates in the irradiated organism.

Animals↗
PubMed1989

[The dynamic morphofunctional state of the adrenals in irradiated recipients following bone marrow transplantation].

The influence of syngenic bone marrow transplantation in the dose of 1.10(7) cell/ml upon the adrenal gland state of lethally irradiated recipients at various stages of posttransplantation period during 3 months has been studied at 150 linear F1 (CBA x C57B) male mice. Histological and histochemical studies conducted have shown the transplantation of native and cryopreserved bone marrow of the lethally irradiated recipients to result, depending upon observation time, in undulating change in adrenal gland weight, cortex thickness and lipid content in spongiocytes, which testifies to the certain dynamics of processes, occurring in recipients' adrenal gland.

Adrenal Glands↗
PubMed1985

[Conservation of bone marrow CFUc in different phases of the cell cycle during cryopreservation].

The effect of low temperature (-196 degrees C) preservation on the recovery of colon-forming units (CFUs) of bone marrow at different phases of the cell cycle before cryopreservation is dealt with. The intact bone marrow "enriched" with CFUs in S phase of the cell cycle and the bone marrow without colony-forming units in S phase were exposed to cryopreservation. After cryopreservation of the bone marrow enriched with CFUs in S phase and th bone marrow without colony-forming units in S phase the number of CFUs decreases by the same value as in the cryopreserved bone marrow obtained from intact mice.

Animals↗
PubMed1982

[Comparative study of the proliferative activity of native and cryopreserved colony-forming units in humans and mice].

The agar culture method was used to state that colony-forming units responsible for cell structures of a hemopoietic series remained undamaged after cryopreservation (-196 degrees) in a suspension of mouse and human donor bone marrow. Cultivation of cryopreserved bone marrow revealed the inhibition of proliferative activity of cells growing in agar as compared with native ones. The inhibition of DNA synthesis was the basis of this process.

Animals↗
PubMed1982

[Respiration activity of frozen-thawed lymphoid cells].

The respiration and oxidative phosphorylation rate of lymphocytes were studied after their cryopreservation. The oxygen uptake of cells frozen in the freezing medium containing 20% serum and 10% PEO-400 or DMSO is higher than that of the native lymphocytes. 2,4-DNP has not demonstrated any stimulating effect on the frozen-thawed cell respiration. Succinate has significantly stimulated the respiration of cells frozen with PEO-400 and produces a slight stimulating effect on the respiration of cells frozen with DMSO. It is concluded that the mitochondria and plasma membranes of cryopreserved lymphocytes undergo considerable changes.

Animals↗
PubMed1980

[Cellular structures formed during bone marrow cultivation in agar].

The growth-enhancing effect and the differentiation of colonies formed by native and frozen-thawed myelokaryocytes in semiliquid agar were observed over a period from the 2nd to the 28th day. The cultivation of bone marrow in agar was shown to form colonies consisting of haemopoietic cells as well as of stromal elements. Under the cultivation of frozen-thawed bone marrow, the retardation of proliferative activity of growing cells was observed within up to 4 days. Later on the proliferative character and the rate of the myelokaryocytes of both the types were seen identical. The question of a possible existence of haemopoietic tissue and stromal elements in the progenitor cells of normal bone marrow is being discussed.

Agar↗
PubMed1979

[Macrophage reaction in the bed of an allogeneic skin transplant].

The character of cell reactions, number of macrophages at the place of skin allograft was demonstrated to depend on the stage of posttransplantational period. Macrophagal reaction increases as the crisis of rejection is approaching; functional and metabolic activity of macrophages is increasing that is evident from an intensive vacuolization of cytoplasm, decreased contents of glycogene, enhanced acid phosphatase activity in them, increased amount of H3-thymidin labelled cells.

Acid Phosphatase↗
PubMed1978

[Nature of intercellular interactions at various stages of post-transplantation immunity].

The paper presents the results of studies on the nature and frequency of intercellular interactions at various stages of posttransplantation immunity done by means of the grating microscope. The study was carried out in inbred mice. The donors of the skin included CBA mice (for the syngeneic system) and BALB/c mice (for the allogeneic system), and the recipients were CBA mice. The target cells were epidermal cells of the tails of mice of the appropriate lines. The interaction of the target cells with lymphocytes, thymocytes and macrophages obtained from the animals sensitized with the appropriate transplant were examined at various intervals after transplantation. The frequency and the nature of intercellular contacts were found to vary in relation to the time after grafting and the species of the graft. Numerous arches, bridges, outgrowths and microvilli were formed both between killer cells and immunocompetent cells and between lymphoid and non-lymphoid cells.

Animals↗
PubMedDate not supplied

[Cryostability of yeast cells and their sensitivity to basic factors of freezing].

The cryostability of Saccharomyces cerevisiae cells was found to correlate with their sensitivity to some physico-chemical factors of freezing. The threshold of their resistance to the main factors of cryoconservation (a low-temperature shock, the presence of a salt, a change in the pH of the medium) was determined at a temperature value physiological for this microorganism. This work was done in order to substantiate and predict optimal freezing regimes while making microbial cells pass into the state of deep cryptobiosis.

Cryopreservation↗
PubMedDate not supplied

[The effect of initial bioenergetic state on the cryostability of yeast cells].

The cryostability of Saccharomyces cerevisiae cells decreased when they were cultivated under anaerobic conditions in a liquid growth medium YEPD as compared to the culture grown under aerobic conditions. The effect of cultivation conditions on the different cryostability of S. cerevisiae cells is discussed. The initial state of their bioenergetics was shown to influence the cryostability of yeast cells.

Anaerobiosis↗
PubMedDate not supplied

[Effect of low temperatures (-196 degrees) and of cryoprotectors on some bacterial species].

The effect of low temperatures (-196 degrees C) and cryoprotectors (PEO-400 and glycerol) on the survival, morphological and functional properties was studied with Escherichia coli, Serratia marcescens and Staphylococcus aureus 209. When the cells were frozen for a short period of time in liquid nitrogen, the survival and the rate of protein synthesis decreased in the gram-negative bacteria but remained almost the same in Staphylococcus aureus. PEO-400 and glycerol manifested cryoprotecting action on all the bacteria under study at concentrations which did not harm the bacteria.

Bacteria↗
PubMedDate not supplied

[Intracellular development of Escherichia coli bacteriophages after culturing the infected bacteria under conditions of anabiosis and hypothermia].

The intracellular growth of bacteriophages T3, T4 and phi X174 was studied in Escherichia coli cells frozen to -196 degrees C and cooled to 0 degree C at various intervals from the instant of phage infection. The processes of biosynthesis were delayed and the latent period was longer in the growth of cells frozen to -196 degrees C. The levels of RNA and protein biosynthesis as well as the yield of phages decreased when cells were frozen at a later stage of the phage growth. No changes were found in the intracellular growth processes of the phages during the subsequent cultivation of the bacterium when it was infected and then cooled to 0 degree C.

Bacterial Proteins↗
PubMedDate not supplied

[Effect of PHA on lymphocyte respiratory activity in mice].

A study of the intensity of oxygen consumption by mice lymphocytes at different stages of cultivation with PHA showed a pronounced increase of the respiratory activity beginning from the 14th hour. Maximum values of oxygen consumption by stimulated cells were observed at the 16th and 30th hours of cultivation (before the beginning of blastogenesis and before the maximum uptake of H3-thymidine, respectively.

Animals↗