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Biomedical subjects

A A Zubakhin

Publications and source records attributed to A A Zubakhin.

At least 19 recordsLinked to original sources
PubMed2004

Reactivity of alveolar macrophages during granulomatous inflammation of the lungs under conditions of acute massive blood loss.

Reactivity of mouse alveolar macrophages by their ability to phagocytize killed St. aureus bacteria and production of reactive oxygen metabolites (nitro blue tetrazolium test) in response to zymosan administration was studied under normal conditions and after acute massive blood loss. Zymosan-induced granulomatous inflammation of the lungs during acute massive blood loss 2-fold inhibited the increase in oxidative metabolism of alveolar macrophages. Suppressed production of toxic oxygen radicals in alveolar macrophages was accompanied by accelerated recovery of cells on the surface of the respiratory tract.

Animals↗
PubMed2000

Liver resistance to CCl(4)-induced injury after stimulation of macrophages with various preparations.

Acute toxic hepatitis in male Wistar rats was produced by single injection of 40% CCl(4) (0.2 ml per 100 g body weight in oil). Pretreatment with various immunostimulators (bacterial polysaccharides prodigiozan and salmozan; yeast polysaccharides zymosan, peptidoglycan, and mannan; and hydrolytic enzyme egg lysozyme) produced a hepatoprotective effect correlating which the stimulatory influence on macrophages and increasing in the following order: mannan<peptidoglycan<zymosan<lysozyme<salmozan<prodigiozan.

Adjuvants, Immunologic↗
PubMed2000

Liver resistance to toxic effects of CCl(4) under conditions of gadolinium chloride depression of Kupffer cells.

Acute toxic hepatitis was modeled in (CBAxC57B1)F1 mice by single injection of 40% CCl(4) in oil. Pretreatment with gadolinium chloride, a selective blocker of Kupffer cells, considerably potentiated damage to hepatocytes leading to generalization of this process, delayed inflammatory infiltration, and inhibited reparative processes. Zymosan administered against the background of gadolinium chloride blockade improved liver resistance to CCl(4)-induced damage, intensified mononuclear infiltration, and accelerated reparative processes.

Animals↗
PubMed1993

Mononuclear phagocyte system responsiveness in CCl4-induced liver cirrhosis.

In rats with CCl4-induced liver cirrhosis, a twofold decrease of blood clearance rate and a fourfold reduction in number of Kupffer cells taking up colloidal carbon particles has been demonstrated. Zymosan stimulation does not lead to granuloma-like structures in the liver of CCl4-cirrhotic rats. In cirrhotic rats, unlike controls, the cathepsin D activity of liver tissue is very little increased by zymosan treatment and there is virtually no increase in collagenolytic activity. The increase in PGE content in cirrhotic rat liver after prodigiosan stimulation was 2.5 times less than in stimulated control animals. In cirrhotic rats, the IL-1 producing capacity of blood monocytes in vitro in response to lipopolysaccharide drops almost fivefold. The total count of bone marrow-derived myeloid colonies in cirrhotic zymosan-stimulated animals was reduced by 1.5-fold whereas in control animals zymosan induced a 1.8-fold increase in the number of myeloid colonies. The number, uptake and nitroblue tetrazolium-reducing capacities of lung, spleen, peritoneal and bone marrow macrophages in animals with liver cirrhosis were only slightly increased in response to zymosan as compared to control animals. The low response of extrahepatic macrophages to stimuli in cirrhotic animals is thought to be due to their premobilization during the development of cirrhosis.

Animals↗
PubMed1992

[Functional state of the hematopoietic system in different stages of CCL4-induced liver fibrosis in mice].

The state of hemopoietic system at different stages of CCL4-induced liver fibrosis in mice and its reaction to stimuli was examined. At early stages of liver fibrosis (2 weeks of CCL4-administration) we observed a disinhibition of granulo-monocytopoiesis. This is manifested in an increase of the number of immature granulocytes, monocytes/macrophages and CFU-GM in the bone marrow. After 24 h of zymosan injection in this stage an increase of both bone marrow CFU-GM and CFU-E, and the rate of serum colony stimulating activity (CSA) occurred. On the contrary, in advanced liver fibrosis (16 weeks of CCL4 administration), the rate of both granulo-monocytopoiesis and erythropoiesis were decreased as compared with the preceding stage of the process and the response to zymosan became perverted: the number of CFU-GM was paradoxically decreased, while the number of CFU-E remained practically unchanged. The rate of serum CSA increased very poorly too. Thus, in advanced liver fibrosis the hemopoietic system is functionally defective and this factor may be important in development of fibrotic process in the liver.

Animals↗
PubMed1987

[Characteristics of the recovery of the erythron after blood loss in prodigiozan-stimulated mice].

The effect of bacterial polysaccharide prodigiozan on recovery after single acute blood loss (2.5 per cent of the body weight) was studied on female mice CBA x C57Bl (F1). When administered intravenously in a dose of 0.5 mg/kg to healthy animals without blood loss prodigiozan had no significant effect on erythropoiesis. Prophylactic administration of the drug 24 hours before blood loss promoted more rapid and intensive mobilization of erythropoiesis: increasing of the levels of 59Fe incorporation into the bone marrow erythroid cells and the number of erythroid cells in the spleen as compared to those in nonstimulated animals. This is probably connected with prodigiozan-mediated stimulation of stem hemopoietic cell proliferation.

Animals↗
PubMedDate not supplied

[Effect of stimulation and inhibition of macrophage functions on hypercholesterolemia in rats].

In this study we investigated the effects of zymosan and prodigiozan, the macrophage stimulators, and GdCl3, a macrophage inhibitor, on blood lipoprotein composition, activities of liver cholesterly ester (CE) metabolising enzymes, incorporation of [14C]cholesterol (C) into bile acids and accumulation and synthesis of CE in peritoneal macrophages (PM) of rats fed with C-enriched diet for 7 days. The increase of number of phagocyte cells quantity in liver and blood colony-stimulating activity in rats pretreated with intravenous injection of zymosan and prodigiozan was accompanied by reduced C content in blood low density and very low density lipoproteins (LDL and VLDL), increase of liver lysosomal CE hydrolase activity (without change of acyl-CoA:C acyltransferase and cytoplasmatic CE hydrolase activities) and incorporation of labeled C into bile acids and decrease of CE formation and accumulation in PM in rats with hypercholesterolemia. In contrast, reduction of phagocyte population in liver caused by intravenous injection of GdCl3 was accompanied by enhancement of C and CE level in blood LDL and VLDL and decrease of lysosomal CE hydrolase activity and incorporation of C into bile acids in liver of C-feeding rats. The data obtained suggest that the stimulation of mononuclear phagocyte system may lead to a decrease of plasma C via activation of LDL and VLDL catabolism and induction of bile acid synthesis in liver.

Animals↗