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A Alam

Publications and source records attributed to A Alam.

86 records · Page 5Linked to original sources
PubMed1980

Characterization of subspecies from a fungal fatty acid synthetase.

Fatty acid synthetase from the filamentous fungus, Aspergillus fumigatus dissociates during gel filtration on Sepharose 6B into two differently sized subspecies with mol. wt. approx. 1.5 x 10(6) and 8 x 10(5). After elution, they readily reform intact molecules, as determined by their enzymic activity (overall synthetase and 3-oxoreductase activities were measured), sedimentation coefficient and appearance in the electron microscope. Synthetase was cross-linked with dimethyl suberimidate and the resultant protein did not dissociate on Sepharose 6B. The two smaller species which were eluted after chromatography of untreated enzyme were also fixed by reaction with this reagent. They did not reform intact molecules of synthetase and were characterized by electron microscopy as large and small circular aggregates; the low molecular weight form also contained tetrameric structures which exhibited cyclic symmetry. The composition of the two species derived during dissociation was, therefore, confirmed as eight and four polypeptides, respectively; each contained polypeptides A and B. It is proposed that the intact fungal synthetase of composition A6B6 comprises three equivalent loops of protein, each of which contain four polypeptides, presumably with composition A2B2; the molecular weights of A and B are 207 000 and 201 000, respectively. During filtration on Sepharose 6B, two such loops remain associated to form a large circle, leaving the other four polypeptides ro rearrange themselves into a small circle or tetramer.

Aspergillus fumigatus↗
PubMed1978

Pregnancy complicated by pelvic abscess.

Tuboovarian abscess in pregnancy is a rare occurrence. The case presented and discussed is one in which a tuboovarian abscess was diagnosed and managed conservatively, with subsequent delivery of a viable infant.

Abscess↗
PubMedDate not supplied

Acute lower respiratory tract infections in hospitalized patients with diarrhea in Dhaka, Bangladesh.

This study focused on 401 children less than 5 years old who were hospitalized with acute lower respiratory tract infection (ALRI) and diarrhea in Dhaka, Bangladesh, and who were investigated for the presence of both bacterial and viral respiratory tract pathogens as well as for selected diarrheal pathogens. The most common manifestations of ALRI were pneumonia (374 cases), bronchiolitis (12 cases), and tracheobronchitis (11 cases). The majority (77%) of the illnesses were in children less than 2 years of age, and 88% of the children were malnourished. A respiratory tract pathogen was identified in 30% of the patients, and a diarrheal pathogen was identified in 34%. The overall case-fatality rate in children with ALRI and diarrhea was 8%. The case-fatality rate was 14% in children with bacterial pneumonia and diarrhea, 3% in those with viral pneumonia and diarrhea, and 14% in malnourished children with shigellosis and ALRI. The most common respiratory tract pathogens were respiratory syncytial virus, Streptococcus pneumoniae, influenza viruses, and Haemophilus influenzae type b.

Acute Disease↗
PubMedDate not supplied

Acute lower respiratory tract infection due to virus among hospitalized children in Dhaka, Bangladesh.

The nature of acute lower respiratory tract infection (ALRI) in hospitalized children and of the associated viral agents was assessed in a study of 601 children less than 5 years old over a 24-month period. Of these children, 80% were less than 24 months of age and the ratio of boys to girls was 1.7:1. Pneumonia (86.5% of cases) was the most frequently observed clinical manifestation. Shedding of virus was detected in 21.1% of the children; the highest rate occurred in infants 0-5 months old (27%) as compared with a rate of only 12.5% in children 25-60 months old. Virus was detected in 33.3%, 32.8%, 21.2%, and 20% of the cases of tracheobronchitis, bronchiolitis, pneumonia, and croup, respectively. Among the viruses detected, 78% were respiratory syncytial virus (RSV) (91% of infections with this virus occurred in children less than 2 years old) and 14.4% were influenza virus types A and B. Of the RSV infections, 61% occurred in infants less than 1 year old. The case-fatality rate was 6.8% overall and was 4.8% in virus-associated cases. No consistent pattern of seasonal occurrence of viral infections was discerned. RSV was detected throughout the year, with increased prevalence from January to April.

Acute Disease↗
PubMedDate not supplied

Rescue from pediatric ECMO with prolonged hybrid intratracheal pulmonary ventilation. A technique for reducing dead space ventilation and preventing ventilator induced lung injury.

Hybrid intratracheal pulmonary ventilation (h-ITPV) is a continuous flow ventilatory technique that uses a "reverse thruster" catheter to redirect the flow of gas away from the carina. We report here the use of h-ITPV in a pediatric patient with acute sickle cell chest syndrome who required venoarterial ECMO support because of refractory hypoxemic respiratory failure. Her ECMO course was complicated by air leaks, coagulopathy, cardiac tamponade, and necrotizing tracheobronchitis. She could be weaned from ECMO only by maintaining high pressure conventional ventilatory support. To prevent ventilator induced barotrauma, we initiated h-ITPV and weaned her from ECMO bypass. After 12 days of h-ITPV, with tidal volumes of 2-3 ml/kg at carinal peak inspiratory pressures of 25-30 cm H2O, the air leaks ceased and h-ITPV was discontinued. Dead space ventilation fraction (VD/VT) as low as 0.29 was achieved with this technique. Post-h-ITPV bronchoscopy displayed a dramatic resolution of the necrotizing tracheobronchitis. The patient survived and was discharged from the hospital. We conclude that the use of hybrid ITPV may facilitate weaning from ECMO to low pressure conventional ventilation and prevent the development of pulmonary barotrauma.

Acid-Base Equilibrium↗