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A Aransay

Publications and source records attributed to A Aransay.

7 recordsLinked to original sources
PubMed2000

Diagnosis of quinolone-resistant Coxiella burnetii strains by PCR-RFLP.

A total of 12 strains of Coxiella burnetii (8 Greek isolates from acute Q-fever patients, two reference strains-Nine Mile and Q212-and two pefloxacin-resistant laboratory strains) were examined for the presence of point mutations in the quinolone resistance determining region (QRDR) of gyrA gene by direct DNA sequencing of the polymerase chain reaction (PCR)-amplified fragments. The gene sequences of all eight Greek isolates and the two reference strains Nine Mile and Q212 [minimal inhibitory concentration (MIC)</= 4 microg/ml] were identical. Direct DNA sequencing of the in vitro-selected resistant strains (MICs to pefloxacin, 8-32 microg/ml) revealed a transition (G-->A) at the corresponding codon 87 of E. coli. This mutation lead to the substitution of Glu (codon GAG) by Lys (codon AAG ). Restriction maps of amplified gyrA gene sequences were determined by GCG Wisconsin PACKAGE, and the MnlI restriction enzyme was found to cut only the sensitive strains sequences and not the resistant ones. The present PCR-RFLP analysis has proved to be a simple, rapid, and useful method for the detection of Coxiella burnetii and, at the same time, for the diagnosis of quinolone-resistant Coxiella burnetii strains.

Amino Acid Sequence↗
PubMed1995

Molecular characterization of the OXA-7 beta-lactamase gene.

The OXA-7 gene, which encodes an oxacillinase, was cloned from plasmid pMG202 of Escherichia coli isolate 7181 (A. A. Medeiros, M. Cohenford, and G. A. Jacoby, Antimicrob. Agents Chemother. 27:715-719, 1985) and sequenced. The nucleotide sequence of the OXA-7 gene was closely related to that of the OXA-10 (PSE-2) gene, with a derived amino acid sequence of the OXA-7 enzyme showing greater than 95% homology with those of OXA-10 and OXA-11.

Amino Acid Sequence↗
PubMed1993

Pyelic benign fibroepithelial polyp in childhood: a case report.

Intraluminal masses within the upper urinary tracts are rare, are almost always benign, and most commonly take the form of fibroepithelial polyps within the ureters. Only exceptionally are these lesions found within the renal pelvis and for that reason may cause diagnostic difficulty. We present a new case of fibroepithelial polyp of the renal pelvis treated successfully in our hospital.

Child↗
PubMed1989

[Surgery on the prepuce for correction of distal hypospadias without chordee].

Distal hypospadias are the most frequent form of all. Many surgical procedures have been described. We present a series of 84 patients treated using a modification of the technique described in 1967 by Saint Aubert, known as preputioplasty. We described the technique employed. Our esthetics and functional results are excellent. There was a very low complication rate: 1.1 percent of fistulas and 2.3 percent of preputial dehiscence. In 4.7 percent of the patients there was a redundant prepuce. The final result is a non-circumcised appearance which is quite important in our sociocultural ambience.

Child↗