Biomedical subjects

A B DeMilo

Publications and source records attributed to A B DeMilo.

13 recordsLinked to original sources

PubMed1999

Rapid high-performance liquid chromatography method to quantitate elaterinide in juice and reconstituted residues from a bitter mutant of hawkesbury watermelon.

A C(18) reversed-phase HPLC method has been developed to analyze the concentration of 2-O-beta-D-glycopyranosyl-cucurbitacin E (synonym, elaterinide) in juice or reconstituted residues of juice derived from a bitter mutant of Hawkesbury watermelon, Citrullus lanatus (Thunb.) Matsum. & Nakai. The method requires only two steps (homogenation and filtration) for sample preparation prior to column injection and, thus, is amenable to processing large numbers of test samples. Percent elaterinide in juice samples from whole fruit, processed in various ways, ranged from 0.0230 to 0.0808%. Error differences in percent elaterinide for repetitive analyses within the same lot of juice ranged from 1.2 to 5.6%.

Beverages↗

PubMed1996

Saturated and monofluoro analogs of the oriental fruit fly attractant methyl eugenol show reduced genotoxic activities in yeast.

Methyl eugenol, is a commercially used fruit fly attractant and a suspected carcinogen. Several phenylpropenes, including methyl eugenol and the known carcinogen safrole, score negative in the Salmonella assay but score positive in the yeast DEL assay that selects for intrachromosomal recombination events in the yeast Saccharomyces cerevisiae. In an attempt to dissociate the beneficial properties of methyl eugenol from its genotoxic properties, saturated or fluorinated analogs were evaluated for their ability to induce intrachromosomal (DEL) recombination in yeast. Field tests have previously shown that all of the analogs used have appreciable properties as fruit fly attractants. The analogs 1,2-dimethoxy-4-ethylbenzene, 1,2-dimethoxy-4-(2-fluoro-2-propenyl)benzene, 1,2-dimethoxy-4-(2-fluoroethyl)benzene and 1,2-dimethoxy-4-(3-fluoro-2-propenyl)benzene all showed reduced toxicity and reduced recombinagenicity in yeast compared to methyl eugenol. These results confirm the validity of fluorination and/or removal of the 2-propenyl moiety in reducing the toxicity and recombinagenicity of methyl eugenol derivatives.

Animals↗

PubMed1988

Susceptibility of laboratory-reared northern fowl mites, Ornithonyssus sylviarum (Acari: Macronyssidae), to selected acaricides.

Toxicity was determined for 15 acaricides against a laboratory strain of northern fowl mites, Ornithonyssus sylviarum (Canestrini and Fanzago). Adult females were exposed to residues on filter paper for 24 h. Three organophosphorous compounds (monocrotophos, cythioate, and famphur) were more toxic to the northern fowl mite than was carbaryl, the most commonly used pesticide in the poultry industry. The other tested compounds were less toxic to the mite than was carbaryl. Four of these, not used previously for northern fowl mite control, had low LC50's for northern fowl mites:aldicarb (0.46); pirimiphos-methyl (0.73); exo, exo-2,8-dichloro-4-thiatricyclo[3.2.1.0.]octane-4-oxide (AI3-63182) (0.87); and diazinon (2.48).

Animals↗

PubMed1977