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A B Kriegler

Publications and source records attributed to A B Kriegler.

26 records · Page 2Linked to original sources
PubMed1986

Subpopulations of mouse bone marrow high-proliferative-potential colony-forming cells.

Bone marrow cells taken from mice treated eight days previously with 5-fluorouracil, formed colonies consisting of 10-100 cells after four days of incubation in methylcellulose cultures containing only 500 cells/dish, in the presence of partially purified synergistic factor from human placental-conditioned medium (SFHPlac) and macrophage colony-stimulating factor (CSF-1). Replating of these colonies revealed a high incidence (27%) of another class of high-proliferative-potential colony-forming cells (HPP-CFC) responsive only to the synergistic factor in WEHI-3B-conditioned medium (SFW, which appears to be identical to interleukin 3) plus CSF-1. These colonies contained no HPP-CFC responsive to SFHPlac plus CSF-1, although primary cultures incubated for 14 days in the presence of SFHPlac plus CSF-1 formed large colonies (diameter greater than 0.5 mm), indicating the presence of HPP-CFC responsive to SFHPlac plus CSF-1 in the starting marrow. Primary cultures containing SFW alone, or purified interleukin 3 alone, also gave rise to colonies consisting of 10-100 cells after four days of incubation in methylcellulose cultures; however, the cells from these colonies were unable to form large colonies on replating in the presence of either CSF-1 plus SFHPlac or CSF-1 plus SFW. These results suggest that two distinct populations of HPP-CFC exist and that the population of HPP-CFC stimulated by CSF-1 plus SFHPlac differentiates to form HPP-CFC that respond to CSF-1 plus SFW.

Animals↗
PubMed1984

Sources of murine macrophage colony-stimulating factor that also contain growth factors for primitive macrophage progenitor cells.

A new growth factor (synergistic factor, SF) has recently been described, which, in combination with a macrophage CSF source, is able to stimulate the proliferation of primitive high-proliferative-potential macrophage-progenitor cells (HPP-CFC) in mouse bone marrow in culture. It has been found that the addition of supraoptimal amounts of the crude CSF sources, extracts of pregnant mouse uterus and embryo, media conditioned by L cells or the mouse mammary tumor cell line (EMT6), stimulated the proliferation of HPP-CFC in the absence of any added SF. These preparations thus appear to contain a factor with similar biological properties to those of SF. This conclusion is supported by the results obtained from Sephacryl S200 chromatography of these three CSF sources, which indicate that in all three cases fractions with apparent molecular weight (MW) greater than 68,000 contained the major portion of the CSF-1 activity, whereas fractions with MW less than 68,000 contained the SF-like activity, together with minor amounts of CSF-1 activity.

Animals↗
PubMed1984

The effect of prostaglandins E1 and E2 on macrophage progenitor cells with high proliferative potential in mouse bone marrow in vitro.

High proliferative potential macrophage progenitor cells (HPP-CFC) in 5-fluorouracil (FU) treated and normal mouse bone marrow (BM) have been shown to be less sensitive to inhibition of proliferation by prostaglandins of the E series (PGE) than low proliferative potential macrophage progenitor cells (LPP-CFC) in normal BM in agar cultures. The growth of large colonies (diameter greater than 0.5 mm) derived from HPP-CFC in FU BM, which require a combination of macrophage colony-stimulating factor (CSF-1) plus a new growth factor called synergistic activity (SA), are inhibited by 50% in the presence of 5.5 X 10(-6) M PGE1. On the other hand, LPP-CFC in normal BM, which form smaller colonies (diameter less than or equal to 0.5 mm) in the presence of CSF-1 alone, require only 5 X 10(-8) M PGE1 for the same level of inhibition. Addition of appropriate concentrations of PGE1 to the agar culture assay should improve detection of HPP-CFC by inhibiting the proliferation of LPP-CFC. These observations suggest that the apparent negative feedback control of macrophage production by PGE operates largely on the LPP-CFC, which respond to CSF-1 alone, and is probably not involved in the regulation of the more primitive HPP-CFC.

Alprostadil↗
PubMed1982

Partial purification and characterization of a growth factor for macrophage progenitor cells with high proliferative potential in mouse bone marrow.

A population of macrophage progenitor cells, with high proliferative potential, has recently been demonstrated in postfluorouracil-treated and normal mouse bone marrow (BM) in vitro, when the newly discovered growth factor (synergistic activity, SA) is combined with a macrophage colony-stimulating factor (CSF) as a proliferative stimulus. SA, shown to be present in human spleen and placental conditioned media (HSCM and HPCM, respectively) have been studied and found to be unstable to trypsin digestion and to heating at 50 degrees C or above; stable between pH 4 and 9; nonadherent to Con-A-Sepharose; and to have an isoelectric point between pH 5 and 5.8 and a molecular weight of between 14,000 and 21,000 as indicated by gel filtration chromatography. SAs from both HSCM and HPCM have been purified 89- and 122-fold, respectively, by precipitation of extraneous proteins at pH 5 followed by chromatographing twice on Sephacryl S200. Neither of these partially purified SAs contain any CSF for mouse BM. These results indicate that the SAs from HSCM and HPCM may be closely related and that they are structurally different from CSFs derived from various murine sources that have been shown to be stable to proteolytic enzymes and heat.

Animals↗
PubMed1981

Identification of the "factor" in erythrocyte lysates which enhances colony growth in agar cultures.

The activity in human erythrocyte lysates which enhances colony growth of mouse bone marrow (BM) and other cell types in agar culture, could not be separated from hemoglobin (Hb). This conclusion was reached after various procedures, including purification of Hb in human hemolysates by crystallisation, separation of Hb into its major (A0) and minor (A1 and A2) components by DEAE-Sephadex chromatography and separation of a hemolysate into a Hb fraction and a non-Hb protein fraction by DEAE-cellulose chromatography; all resulted in the enchancement activity remaining with the Hb fraction. Separation of globins from rat or human lysates by an acid acetone precipitation, resulted in an acetone powder (AP) which retained the enhancement activity towards both mouse BM and tumour cell lines. The AP was separated into alpha and beta globins by chromatography on Sephadex G100 in 20% formic acid followed by CM-cellulose chromatography in a 8 M urea system. Since the enhancement activity is associated with both the alpha and beta globin peaks even under these dissociating conditions, it has been concluded that the enhancement factor in erythrocyte lysates is Hb itself. The enhancement activity of an AP is abolished by treatment with N-ethylmaleimide, suggesting that sulfhydryl groups in Hb are required for the activity.

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