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Biomedical subjects

A B Pandit

Publications and source records attributed to A B Pandit.

18 recordsLinked to original sources
PubMed2006

Sonocrystallization: effect on lactose recovery and crystal habit.

Sonocrystallization is the use of power ultrasound to control the crystallization process, commonly used during the nucleation phase of crystallization. However, in the present study a different approach has been tried, in which the whole process of lactose crystallization from model reconstituted lactose solutions was completed rapidly with the aid of ultrasound, in the presence of 'ethanol' as an anti-solvent, at temperature of 30+/-2 degrees C (ambient temperature). The lactose recovery and crystal properties from sonicated samples were compared with non-sonicated samples. For optimization of sonocrystallization process for rapid lactose recovery, variations in the time of sonication, lactose concentration, protein concentration and pH were tried. A lactose recovery of 91.48% was obtained in 5 min of sonication time, from a reconstituted lactose solution (17.5% w/v, pH 4.2) as against 14.63% under only stirring. Lactose recovery decreased with lowering of pH from 4.2 to 2.8. The protein showed maximum influence on lactose recovery even at concentration of 0.2% w/v. A rapid process of crystallization gave a better uniformity in crystal size distribution of lactose samples.

Crystallization↗
PubMed2005

Ultrasonic degradation of poly(vinyl alcohol) in aqueous solution.

Solution of poly(vinyl alcohol) in water with different concentrations (by weight 1%, 1.5%, 2%) and different volumes (50, 75 and 100 ml) were subjected to ultrasonic degradation. A method of viscometry was used to study the degradation behavior and kinetic model was developed to estimate the degradation rate constant. The degradation rate constant was correlated with the power input due to ultrasonic irradiation and reaction volume. It was found that rate constant decreases as the reaction volume and concentration increases. The proportionality index of the relation between rate constant, power input and reaction volume was found to be nearly equal for all concentrations studied. The proportionality constant was found to be approximately equal for 1% and 1.5% solution and for 2% solution it was approximately half the value for that of 1% and 1.5% solutions. The decrease in rate constant and proportionality constant is attributed to the fact that at higher concentration and at higher volume, the intensity of cavitation phenomenon is depressed and therefore the extent of polymer chain breaking decreases. The difference in the values of limiting viscosities (constant solution viscosity which does not decrease by further ultrasonic irradiation) for 50, 75 and 100 ml solutions for each of 1% and 1.5% concentration was negligible. But 2% solution at 100 ml volume showed slightly higher value of limiting viscosity than that for 50 and 75 ml.

Journal Article↗
PubMed2005

Transdermal drug delivery using ultrasound-theory, understanding and critical analysis.

This review focuses on a unique transdermal drug delivery enhanced by the action of ultrasound, referred as sonophoresis. Sonophoresis is an active form of transdermal delivery which enhances the transport of permeants, such as drugs through cell membranes as a result of ultrasonic energy. Ultrasonic sound waves cause acoustic cavitation, the resultant effects of which microscopically disrupt the lipid bilayers of the stratum corneum and thereby influencing the influx of permeants. Sonophoresis increases the penetration of various low molecular weight drugs as well as high molecular weight proteins. The objective of this review is to account the role of ultrasound parameters and the associated cavitational effects, gained through a number of investigations, in order to facilitate the understanding of this method.

Administration, Cutaneous↗
PubMed2005

Oxidation of alkylarenes using aqueous potassium permanganate under cavitation: comparison of acoustic and hydrodynamic techniques.

Various alkylarenes were oxidized to the corresponding aryl carboxylic acids using aqueous potassium permanganate under heterogeneous condition in the presence of hydrodynamic cavitation and the results of the reaction have been compared with the acoustic cavitation in terms of their energy efficiency. The rate of reaction was determined for each reaction. In the oxidation of p-xylene, seven times more product could be obtained in the case of hydrodynamic cavitation than in the case of acoustic cavitation. The reaction was found to be considerably accelerated at ambient temperature.

Alkylation↗
PubMed2004

Oxidation of alkylarenes to the corresponding acids using aqueous potassium permanganate by hydrodynamic cavitation.

Oxidation of toluene using aqueous potassium permanganate was studied under heterogeneous condition in the presence of hydrodynamic cavitation and compared with the results of the reaction under acoustic cavitation. Various parameters, such as quantity of potassium permanganate, toluene to aqueous phase ratio, reaction time and cavitation parameters such as orifice plate, and pump discharge pressure were optimized. The reaction was found to be considerably accelerated at ambient temperature in the presence of cavitation. On comparison, it was found that when 1 kJ of energy was passed to the reaction mixture in the case of acoustic cavitation, the product obtained was 4.63 x 10(-6) mol, whereas when 1 kJ of energy was passed to the reaction mixture in the case of hydrodynamic cavitation the product obtained was 2.70 x 10(-5) mol. Hence, about six times more product would be obtained in the case of hydrodynamic cavitation than in the case of acoustic cavitation at same energy dissipation. It has been observed that further optimization is possible.

Journal Article↗
PubMed2004

Effect of cavitation on chemical disinfection efficiency.

This study brings out the potential efficacy of hybrid techniques for water disinfection. The techniques studied include hydrodynamic cavitation, acoustic cavitation and treatment with chemicals like hydrogen peroxide and ozone. The hybrid techniques which combine hydrodynamic cavitation, acoustic cavitation and hydrogen peroxide appear to be an attractive alternative to any one technique on its own for the reduction in the heterotropic plate count bacteria as well as indicator microorganisms like the total coliforms, fecal coliforms and fecal streptococci.

Colony Count, Microbial↗
PubMed2003

Hybrid cavitation methods for water disinfection: simultaneous use of chemicals with cavitation.

This study brings out the potential efficacy of hybrid techniques for water disinfection. The techniques studied include, hydrodynamic cavitation, acoustic cavitation and treatment with chemicals such as ozone and hydrogen peroxide. The phenomena of cavitation which involves formation, growth and violent collapse of vapor bubbles in a liquid media is known to generate a high intensity pressure which affects the cell and microorganism viability. The hybrid technique which combines hydrodynamic cavitation, acoustic cavitation, hydrogen peroxide and/or ozone appears to be an attractive alternative to a single technique for the reduction in the heterotropic plate count bacteria as well as indicator microorganisms like the Total coliforms, Fecal coliforms and Fecal streptococci.

Disinfection↗
PubMed2002

Ultrasound mediated alkaline hydrolysis of methyl benzoate--reinvestigation with crucial parameters.

In the present work hydrolysis of methyl benzoate was carried out using aqueous sodium hydroxide solution at room temperature in the presence of ultrasound since otherwise the same reaction takes place at relatively high temperature. Also, the above hydrolysis reaction was investigated at a relatively larger scale with the variation in parameters influencing the emulsification process and hence the reaction rates. It has been observed that the position of ultrasound source on the liquid-liquid interface is a crucial parameter affecting the two-phase emulsification rates. The poor bulk mixing occurring in the presence of ultrasound alone with an increase in the volume of the reaction mixture and its consequent effect on the reaction kinetics has been conclusively established. These studies have shown that the use of ultrasound with mechanical stirring can result in substantial reduction in the overall power consumption, especially for reaction systems like hydrolysis that do not require very high temperatures and pressures generated by cavitation.

Journal Article↗
PubMed2001

Significance of location of enzymes on their release during microbial cell disruption.

The release kinetics of the enzyme invertase and alcohol dehydrogenase from yeast and penicillin acylase from E. coli during disruption using various techniques has been investigated. The disruption techniques used were sonication, high-pressure homogenization, and hydrodynamic cavitation. The first-order-release kinetics was applied for the determination of release rate of these enzymes and total soluble proteins. Location factor (LF) values were calculated using these release rates. The location of the enzymes as given by the values of location factor coincided well with those reported in the literature. Varying values of location factor for the same enzyme by different disruption techniques gave some indications about the selectivity of release of a target enzyme by different disruption techniques. Varying values of location factor for the same enzyme with the use of a particular equipment or disruption technique at different conditions reveals the degree to which the cell is disrupted. Few plausible applications of this location factor concept have been predicted and these speculations have been examined. This location factor concept has been used for monitoring the heat-induced translocation of ADH and location of penicillin acylase during the growth period of E. coli cells.

Alcohol Dehydrogenase↗
PubMed2001

Correlations to predict droplet size in ultrasonic atomisation.

In conventional two fluid nozzles, the high velocity air imparts its energy to the liquid and disrupts the liquid sheet into droplets. If the energy for liquid sheet fragmentation can be supplied by the use of ultrasonic energy, finer droplets with high sphericity and uniform size distribution can be achieved. The other advantage of ultrasound induced atomisation process is the lower momentum associated with ejected droplets compared to the momentum carried by the droplets formed using conventional nozzles. This has advantage in coating and granulation processes. An ultrasonic probe sonicator was designed with a facility for liquid feed arrangement and was used to atomise the liquid into droplets. An ingenious method of droplet measurement was attempted by capturing the droplets on a filter paper (size variation with regard to wicking was uniform in all cases) and these are subjected to image analysis to obtain the droplet sizes. This procedure was evaluated by high-speed photography of droplets ejected at one particular experimental condition and these were image analysed. The correlations proposed in the literature to predict droplet sizes using ultrasound do not take into account all the relevant parameters. In this work, a truly universal correlation is proposed which accounts for the effects of physico-chemical properties of the liquid (flow rate, viscosity, density and surface tension), and ultrasonic properties like amplitude, frequency and the area of vibrating surface. The significant contribution of this work is to define dimensionless numbers incorporating ultrasonic parameters, taking cue from the conventional numbers that define the significance of different forces involved in droplet formation. The universal correlations proposed are robust and can be used for designing ultrasonic atomisers for different applications. Among the correlations proposed here, those ones that are based on the dimensionless numbers and Davies approach predict droplet sizes within acceptable limits of deviation. Also, an empirical correlation from experimental data has been proposed in this work.

Humans↗
PubMed2001

Ultrasonic degradation of 2:4:6 trichlorophenol in presence of TiO2 catalyst.

The degradation of trichlorophenol has been studied at different intensities of irradiation using ultrasonic horn by changing the power input to the system. Effect of presence of catalyst TiO2 and concentration of catalyst on the degradation rates has also been investigated. The rates of degradation are found to be higher at higher intensities in the absence of catalyst but reverse trend is observed in the presence of catalyst. Adsorption and desorption characteristics of trichlorophenol on TiO2 catalyst have been examined. The catalyst enhances the rates of degradation but it also adsorbs some amount of TCP during the degradation process protecting it from ultrasonic degradation. Thus it is essential to consider the adsorption and desorption kinetics to get proper estimates of the degradation rates when the rates of degradation of TCP are calculated by analyzing the residual concentration of the compound in the liquid. The degradation of the pollutant seems to take place in the liquid only and that too only after desorption of the same from the solid particles. Solid particles seem to play a physical role in the overall degradation scheme, providing additional surface cavitation.

Journal Article↗
PubMed2001

Ultrasound enhanced degradation of Rhodamine B: optimization with power density.

Degradation of Rhodamine B, a waste water dye effluent was studied under the influence of ultrasound. In the present study, optimization of this degradation was carried out with power parameters, namely, power density (W/ml) and power intensity (W/cm2) using different ultrasonic equipments. From the studies, it has been observed that the degradation does not increase indefinitely with an increase in the power parameters, but instead, it reaches an optimum value and then decreases with a further increase in the power parameters. Also, these optima were not the same in all the ultrasonic equipments in which these studies were carried out. Difference in the type of acoustic field generated may be responsible for the different power optima observed with different ultrasonic equipments. The comparative study of the degradation of Rhodamine B using various ultrasonic equipments establishes a relationship between the area-specific parameter (intensity) and the volume-specific parameter (density) of reactivity.

Journal Article↗
PubMed2001

Mixing time analysis of a sonochemical reactor.

Mixing time measurements have been carried out in a cylindrical reactor irradiated with an ultrasonic horn fitted with different size tips. Liquid phase bulk velocities induced by the vibrating horn surface have been estimated from the mixing time measurements. A relationship has been established between the mean horn surface velocities (frequency x amplitude) and the mean velocities estimated from the mixing time measurements. A correlation has been developed for the prediction of the mixing time using a method similar to that used for liquid jet mixing. This could be the first step in defining the overall flow field, the information about which can then be used to get realistic numerical solutions of the Rayleigh-Plesset equation for a travelling cavity to understand the cavity dynamics in the various parts of the ultrasonic horn reactor.

Journal Article↗
PubMed1999

Hydrodynamic cavitation for sonochemical effects.

A comparative study of hydrodynamic and acoustic cavitation has been made on the basis of numerical solutions of the Rayleigh-Plesset equation. The bubble/cavity behaviour has been studied under both acoustic and hydrodynamic cavitation conditions. The effect of varying pressure fields on the collapse of the cavity (sinusoidal for acoustic and linear for hydrodynamic) and also on the latter's dynamic behaviour has been studied. The variations of parameters such as initial cavity size, intensity of the acoustic field and irradiation frequency in the case of acoustic cavitation, and initial cavity size, final recovery pressure and time for pressure recovery in the case of hydrodynamic cavitation, have been found to have significant effects on cavity/bubble dynamics. The simulations reveal that the bubble/cavity collapsing behaviour in the case of hydrodynamic cavitation is accompanied by a large number of pressure pulses of relatively smaller magnitude, compared with just one or two pulses under acoustic cavitation. It has been shown that hydrodynamic cavitation offers greater control over operating parameters and the resultant cavitation intensity. Finally, a brief summary of the experimental results on the oxidation of aqueous KI solution with a hydrodynamic cavitation set-up is given which supports the conclusion of this numerical study. The methodology presented allows one to manipulate and optimise of specific process, either physical or chemical.

Journal Article↗
PubMed1999

Purification of alpha-glucosidae and invertase from bakers' yeast on modified polymeric supports.

In the present work Amberlite XAD-16 and Indion NPA-1, Polystyrene Divinylbenzene macroreticular spherical resins, have been evaluated quantitatively as supports for the adsorption and isolation of the yeast proteins and the enzymes, invertase and alpha-glucosidase. Modification of these supports has been carried out by surface grafting using acrylate polymers to reduce the hydrophobicity and nonspecific adsorption of proteins. Good grafting efficiency, in excess of 90%, has been obtained using ultrasonic irradiation for the surface activation of polystyrene resins. XAD-16 has higher adsorption capacities for the total yeast proteins as well as for both the enzymes, alpha-glucosidase and invertase, than NPA-1 in its respective native and grafted form. Adsorption capacities of XAD-16 and NPA-1 in their respective native and grafted forms for alpha-glucosidase are higher than the capacities for invertase. Nonspecific adsorption of total proteins has been reduced considerably after the grafting of acrylate polymers on hydrophobic supports. At the same time selectivity for the adsorption of both the enzymes has been enhanced on grafted supports. The overall solid-liquid adsorption mass transfer coefficient values (Kla) estimated for adsorption of invertase on XAD are lower than those for alpha-glucosidase. Native and grafted resins could be regenerated and reused for adsorption of alpha-glucosidase for two regeneration cycles studied. Storage stability of invertase and alpha-glucosidase is the same on native and grafted form of XAD-16 and is more than the enzymes in the free form.

Enzyme Stability↗
PubMed1998

Sonophotochemical destruction of aqueous solution of 2,4,6-trichlorophenol.

The combination of ultrasound and photochemistry have been used to degrade an aqueous solution of 2,4,6-trichlorophenol. An ultrasonic probe of 22 kHz frequency and a UV tube of 15 W have been used. Anatase grade TiO2 was used as the semiconductor catalyst. The effect of parameters such as ultrasonic intensity, operating conditions, type of ultrasonic equipment, and mode of UV transmission have been studied. The sonophotochemical degradation has been found to be dependent on the intensity of sonication, temperature of the reaction, and the type of ultrasonic equipment used, but was independent of the mode of UV transmission. Enhancement in the degradation rate has been observed at a higher sonication intensity and temperature of the solution.

Chlorophenols↗
PubMed1998

Effect of liquid-phase properties on ultrasound intensity and cavitational activity.

The intensity of ultrasound is attenuated due to various properties of the liquid, such as viscosity, density, etc. In this paper, a simple method is proposed to measure the combined attenuation and cavitational activity of ultrasound intensity in various organic liquids using standard KI decomposition reaction. A modified experimental attenuation coefficient is proposed and its dependence on liquid viscosity reasonably matches the theoretical predictions made by Stokes [G.G. Stokes, Trans. Camb. Philos. Soc. 8 (1849) 287]. Exploratory work to determine the effect of other liquid properties on cavitational activity is carried out. Correlations are proposed to explain the dependence of the attenuated cavitational activity on various properties of a liquid.

Journal Article↗