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Biomedical subjects

A B Svendsen

Publications and source records attributed to A B Svendsen.

At least 19 recordsLinked to original sources
PubMed1995

Coupling of the human Y2 receptor for neuropeptide Y and peptide YY to guanine nucleotide inhibitory proteins in permeabilized SMS-KAN cells.

Using guanine nucleotides, pertussis toxin, and specific antisera against the COOH-terminals of the alpha-subunits of Gi1/2, Gi3, and G(o), the binding and biological response of the Y2 receptor (Y2R) for peptide YY (PYY) was probed in SMS-KAN neuroblastoma cells. The specific binding of radiolabeled PYY exhibited a single apparent dissociation constant, KD = 76 pM for intact cells and KD = 906 pM for permeabilized cells. However, other data suggested existence of multiple receptor affinity states. A shift in KD and a decrease in apparent number of binding sites (Bmax) was observed in permeabilized cells when incubated with guanine nucleotides. By contrast, in membrane preparations guanine nucleotides induced only a decrease in Bmax. In intact cells, agonist exposure inhibited the intracellular accumulation of forskolin-stimulated cyclic AMP by 80% (IC50 = 420 nM) compared with 94% inhibition (IC50 = 380 nM) in permeabilized cells. In permeabilized cells, preincubation with antisera against alpha i1/2 and alpha i3 blocked the functional response of PYY, with anti-alpha i3 being the most potent; whereas anti-alpha o failed to affect the cyclic AMP levels. These results suggest that permeabilized SMS-KAN cells serve as a good model system for analysis of Y2R binding kinetics and functional response and that the Y2R interacts directly with several different GiS (but not G(o)).

Cell Membrane Permeability↗
PubMed1989

[Access to psychiatric and child psychiatric case records in the Copenhagen County Hospital in Nordvang in 1987].

Commencing on 1.1.1987, all requests for access to case records were registered prospectively in all of the Copenhagen County Hospitals. In the Copenhagen County Hospital, Nordvang, 103 requests were made for access to the case records in the course of 1987. Ninety-five requests in the adult departments resulted in complete access in 90 cases, limited access in one case while access was refused in three cases. One person making the request did not turn up. Eight requests concerning patients in the child psychiatric department resulted in complete access in one case, limited access in five cases while access was refused in two cases. This corresponds to 3.0% in the departments for child psychiatry and 2.7% in the departments for adults. No deterioration in health was observed in any of the 97 cases where access was permitted. In many cases, insight into the case records may, on the other hand, be a good therapeutic measure. On two cases only, access to the case records was given to a third person with the patient's permission, and the majority of the patients did not request a copy of the case records but accepted access to the case records in the form of the right to read the case records with assistance, if necessary. It is concluded that in the first year after introduction of the Danish legislation concerning access to the case records, this ran very smoothly with fewer difficulties than anticipated in a large psychiatric hospital in the region of the capital.

Child↗
PubMed1984

Tabernaemontana L. (Apocynaceae): a review of its taxonomy, phytochemistry, ethnobotany and pharmacology.

The taxonomy, phytochemistry, ethnobotany, and pharmacology of the genus Tabernaemontana L. (Apocynaceae) is reviewed. The genus is currently being revised taxonomically; most of the segregate genera are being reunited with it and the number of species that will ultimately be recognized will probably be about 100. All the names encountered in the chemical and ethnobotanical literature have been evaluated as far as possible, and a list is presented of the recognized species and their synonyms. The biogenesis and classification of the indole alkaloids found in Tabernaemontana species is set out and some problems in the determination of their stereochemistry are discussed. To facilitate access to the information, three lists have been compiled: the alkaloids in alphabetical order; the alkaloids in order of increasing molecular weight; and the alkaloids grouped according to their biogenetic classification, together with the species and plant part(s) in which they are known to occur. Biogenetic and chemotaxonomic aspects are briefly considered. A table of the non-alkaloidal constituents is also included. The ethnobotany of individual Tabernaemontana species is outlined and an overall assessment made. Likewise, information on the pharmacology of crude extracts and individual alkaloids from Tabernaemontana species has been assembled and appraised.

Alkaloids↗
PubMed1979

Gas-liquid chromatography of digitoxigenin and digoxigenin as acetates of their epoxygeninic acid methyl esters.

Gas chromatographic analysis of ditigoxigenin and digoxigenin, the genins of the cardenolide glycosides digitoxin and digoxin, cannot be done without derivatization. However, during the derivatization, side-reactions often present serious problems. A procedure has been found for transforming digitoxigenin and digoxigenin into the corresponding acetates of their epoxygeninic acid methyl esters, which are stable compounds and suitable for gas chromatographic analysis.

Acetates↗