PubMed Health⌕ Search

Biomedical subjects

A B Thiermann

Publications and source records attributed to A B Thiermann.

40 records · Page 3Linked to original sources
PubMed1980

Canine leptospirosis in Detroit.

Among 433 stray dogs in Detroit, Michigan, surveyed for leptospiral agglutinins, 164 (37.8%) had significant titers to 1 or more leptospiral serotypes; from 123 suburban stray dogs tested, 23 (18.7%) had significant titers. The use of 2-mercaptoethanol on positive serum samples was helpful in identifying acute from chronic phase titers. Cross-reaction patterns also were clarified by the use of 2-mercaptoethanol. Cultural isolation of leptospires from dog urine was unsuccessful unless the urine had a pH from 6.6 to 8.0. Oral administration of sodium bicarbonate to alkalinize dog's urine resulted in the isolation of leptospires from dogs usually voiding acidic urine. Leptospira icterohaemorrhagiae was the most common serotype in this dog population by serologic techniques and was the only one isolated in culture.

Administration, Oral↗
PubMed1980

Human leptospirosis in Detroit and the role of rats as chronic carriers.

Human leptospirosis was diagnosed in three patients from Detroit General Hospital. Epidemiological studies of these cases revealed heavy rat infestations in the residences of two of the patients. Leptospires serotype icterohaemorrhagiae were isolated from the rats and one of the patients. Cultural isolation was attempted from numerous blood and urine samples from each patient. Media containing different selective contaminant inhibitor substances was also utilized. Only two samples were culturally positive. It appears that very few viable cells are shed by human patients intermittently. Clinical reports and leptospirosis diagnosis are reported for each of the cases. The agglutinin-absortion test proved to be helpful in elucidating the true etiologic agent in the absence of cultural isolation. A serological survey for leptospirosis was conducted among swine slaughterhouse workers in Detroit. Significant titres to Leptospira pomona were observed.

Animals↗
PubMed1977

Incidence of leptospirosis in the detroit rat population.

The rat population (Rattus norvegicus) in the city of Detroit was surveyed to determine the incidence of leptospirosis. Four methods of survey were used--serological, direct examination of urine, culture of urine, kidney and brain, and histopathological examination. Of 358 sera tested by the microscopic-agglutination test, 277 (77.4%) had significant agglutinins for serotype icterohaemorrhagiae. Significant differences were found between adult and young animals in both incidence and antibody titers, titers of 1:50 or higher being present in 90.3% and 38.8%, respectively, of adult and young rats. Examination of freshly drawn urine by dark field microscopy revealed that 59.6% of the animals were shedding leptospirae. When stained with Steiner's silver stain, 91.9% of the kidney sections were positive for leptospirae, showing this to be the most sensitive test of those used. Leptospirae were isolated from one or more cultures of kidney, urine, or brain from 221 (59.2%) of the 358 rats examined.

Animals↗