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Biomedical subjects

A B Wilson

Publications and source records attributed to A B Wilson.

At least 19 recordsLinked to original sources
PubMed1991

Lateral rotatory dislocation of the ankle without fracture.

A 34-year-old woman sustained a closed lateral rotatory dislocation of the ankle without a fracture. Treatment was by closed reduction and cast immobilization for 6 weeks. After removal of the cast, her ankle was stable. Stress radiographs of both the affected and contralateral ankles were normal. One year after injury the patient demonstrated a full, pain-free, stable range of motion. The patient exhibited generalized ligamentous laxity, which may have contributed to her tendency to dislocate the talus within the mortise.

Adult↗
PubMed1988

Interleukin-2-induced production of interferon-gamma by resting human T cells and large granular lymphocytes: requirement for accessory cell factors, including interleukin-1.

Between 5 and 20% of normal human lymphocytes were found to synthesize interferon-gamma (IFN-gamma) in primary cultures with recombinant interleukin-2 (rIL-2). After 22 hr, IFN-gamma-producing cells included CD5+ T lymphocytes, CD16+ large granular lymphocytes (LGL), and a population of CD5-, CD16- blast cells. Only a small proportion (0-7%) of IFN-gamma-synthesizing cells expressed HLA-DR. The production of IFN-gamma by all rIL-2-responding lymphocyte subsets was shown to require the presence of DR+ accessory cells, probably including nonadherent, esterase-negative monocytes and/or dendritic cells. Accessory cell function in lymphocyte preparations depleted of DR+ cells, or in purified (greater than or equal to 95%) suspensions of LGL, was fully replaced either by addition of 2% autologous, adherent monocytes or by monocyte culture supernatant. The activity of monocyte supernatant was greatly reduced by treatment with antiserum specific for human interleukin-1 beta (IL-1 beta), although a combination of rIL-1 beta and rIL-2 failed to stimulate IFN-gamma production in DR- lymphocytes. These results indicate that rIL-2-induced IFN-gamma synthesis in both T cells and LGL requires the synergistic activity of IL-1, and possibly of one or more other monokines, as yet unidentified.

Antigen-Presenting Cells↗
PubMed1986

Direct antibody rosette-forming reactions using monoclonal markers of lymphocyte subpopulations. Methodology and applications illustrated by investigations with rat pan-T antibodies of the CAMPATH series.

A sensitive direct antibody rosette assay has been developed for the detection of antigens on the lymphocyte cell membrane. Indicator cells for rosette tests were prepared by chromic chloride coupling of rat or mouse monoclonal IgG or IgM anti-lymphocyte antibodies to untreated or trypsinized bovine red blood cells. The monoclonal antibodies used were reactive with a range of cell surface markers which identify various lymphocyte subpopulations, including T cell antigens, HLA class II (Ia-like antigens), Leu-7 (HNK-1) and VEP 13, a determinant of Fc gamma receptors on large granular lymphocytes. Results obtained by direct rosette formation correlated well with those of parallel tests using indirect immunofluorescent antibodies staining. Several applications of the direct antibody rosetting procedure are described in further investigations with a series of pan-T monoclonal (CAMPATH) antibodies. These include the morphological examination of antibody-binding cells in cytocentrifuge smears, the separation of lymphocyte subsets by density gradient centrifugation, and the use of a rosette inhibition assay to identify monoclonal antibodies binding to the same (or closely associated) epitopes of the lymphocyte cell membrane.

Animals↗
PubMed1986

An in vivo and in vitro study of selenium deficiency and infection in rats.

Selenium deficiency in rats impairs the ability of neutrophils and peritoneal macrophages to kill Candida albicans organisms in vitro. In contrast, killing of Salmonella typhimurium and Staphylococcus aureus organisms is unaffected by the deficiency. Survival of rats after intraperitoneal injection of 8 X 10(7) S. aureus organisms was not affected by Se deficiency, but a 5-fold increase in the dose (4 X 10(8) S. aureus organisms) led to a significantly greater mortality in the Se deficient rats.

Animals↗
PubMed1985

Fc gamma-receptor-bearing, non-B lymphocytes in human peripheral blood: cytophilic immunoglobulin binds almost exclusively to large granular lymphocytes.

Cytophilic IgG (CYT-Ig) has previously been reported to bind to both the "TG" (E+, Fc gamma R+) and "L" (E-, Fc gamma R+) subsets of non-B lymphocytes in human peripheral blood. Present investigations show that IgG-binding cells, as detected by a sensitive antiglobulin rosetting reaction, are contained almost entirely within the large granular lymphocyte (LGL) subpopulation, and that fewer than 5% of other non-B lymphocytes acquire IgG from serum. Cell membrane-bound IgG sterically blocks the reaction of LGL with sheep red blood cells and therefore influences the proportions of these cells characterized as TG (E+) or L (E-) lymphocytes. Although the majority of TG lymphocytes are LGL, a further subpopulation of E+, Fc gamma R+ cells are detectable under particular test conditions. Unlike LGL, these lymphocytes do not react with rabbit IgG-coated ox RBC (EAG) in saline, but will form EAG rosettes when the reaction is enhanced in the presence of Ficoll. These Fc gamma R+ cells are mostly of typical small-lymphocyte morphology and do not bind detectable amounts of CYT-Ig, nor do they express the monoclonal antibody-defined VEP 13 determinant associated with Fc gamma R on LGL.

Antibody Affinity↗
PubMed1979

The Primary Mental Health Project (PMHP): evaluation of current program effectiveness.

This study evaluated the effectiveness of the Primary Mental Project (PMHP), a program for early detection and prevention of school adjustment problems. Pre- and postprogram assessments were done with 215 primary-grade children seen in PMHP, usig teacher ratings of problem behaviors and competencies, and child-aide ratings of problems. School mental health professionals judged educational and behavioral changes in project children during the year. Significant across-the board improvements were found on all criterion measures. Modest intercorrelations among criterion change estimates suggested that the observed changes were due to program, rather than halo, effects. PMHP children also improved significantly more than matched, retrospective controls.

Child↗
PubMed1979

Hairdressers as caregivers. I. A descriptive profile of interpersonal help-giving involvements.

The interpersonal help-giving behaviors of 90 hairdressers were explored in depth in an interview study. On the average, hairdressers saw 55 customers a week, and talked 25 minutes with each. About one-third of the talking time concerned clients' moderate to serious personal problems--particularly problems with children, physical health, marriage, depression, and anxiety. Hairdressers reported that offering sympathy and support, being lighthearted, just listening, and presenting alternatives were among their most frequent response strategies. Although they often enjoyed fielding clients' personal problems, at times they felt perplexed by them. Hairdressers perceived listening to customers' interpersonal problems to be an important part of their everyday function and expressed a need for professional inputs in that domain.

Adult↗
PubMed1979

The evaluation of an intervention program for young schoolchildren with acting-out problems.

The rationale and nature of a program to train nonprofessional child-aides for helping interactions with young acting-out school children are described. Pre/post teacher measures of children's problems and competencies, aide measures of problem behavior, and school mental health professionals' change in behavior estimates were used to evaluate the program's effectiveness. Children seen by trained aides showed significantly greater reductions both in acting-out problems and in overall maladjustment than did similar children seen by comparable aides who did not have additional training, or by themselves before training. Implications were considered for optimizing outcomes both in the specific school intervention project in question and in other, broader types of clinical interventions.

Acting Out↗
PubMed1979

In vitro complement activation by rabbit lymphocytes and thymocytes in autologous serum.

The activation of C3, previously demonstrated to occur during the isolation of lymphocytes from rabbit blood, has been investigated further by in vitro exposure of blood and lymph node lymphocytes and thymocytes to autologous serum. Activated C3 deposited on the lymphoid cell-surface was detected by (i) anti-C3, (ii) immune adherence reaction, and (iii) blocking of receptors for C3. An affinity shown by guinea-pig erythrocytes for activated complement was also investigated. Using EGTA, which inhibits the classical complement pathway while leaving the alternative pathway unaffected, it was shown that either pathway may be activated by rabbit cells depending on the temperature of incubation. The classical pathway was triggered at 4 degrees, probably by the reaction of cold auto-antibodies with lymphoid cell-surface determinants. At 37 degrees, however, comlement activation followed the alternative pathway in a similar manner to that reported by other workers with human lymphoblastoid cell lines.

Animals↗
PubMed1979

Receptors for activated C3 on thymus-dependent (T) lymphocytes of normal guinea-pigs.

In a survey of lymphocyte subpopulations in normal guinea-pig blood, lymph node, spleen, thymus and peritoneal cavity, a considerable overlap was observed between the percentages of C3-receptor bearing lymphocytes (CRL) and of thymus-dependent (T) cells in lymph nodes. Simultaneous rosette-formation reactions with sheep erythrocytes carrying rabbit complement (EAC) and papain-treated rabbit erythrocytes (a T-cell marker) revealed that 20--50% of the lymph node CRL were T lymphocytes. These experiments and others on cell suspensions depleted of Ig-bearing (B) lymphocytes showed that between 8 and 36% of lymph node T cells have complement receptors. The frequency of T-CRL in other lymphoid tissues was lower, representing between 0 and 8% of the T-cell population. The reaction of T-CRL and EAC was not inhibited by EDTA which is known to inhibit the C3 receptor activity on macrophages.

Animals↗