Biomedical subjects

A Bártová

Publications and source records attributed to A Bártová.

At least 19 recordsLinked to original sources

PubMed1998

Class I typing by PCR-SSP in Olomouc.

In this article, methodical experience and population data are given for application of PCR-SSP/ARMS method (Phototyping) to HLA class I typing in Olomouc. Phototyping is reliable method suitable for typing of small to medium number of samples. Method is fast enough for use in on-call service, resolution is better than the level of good serology, and price of method is comparable with serology. Our experience and tips are described below. Population data of healthy unrelated individuals for HLA-A, -B, -Cw are given in the tables.

Gene Frequency↗

PubMed1997

Familial hydronephrosis unlinked to the HLA complex.

Clinical findings, management, and possible linkage of congenital hydronephrosis caused by ureteropelvic junction stenosis to the HLA complex were studied in four families. These families provide evidence of possible autosomal dominant inheritance. HLA class I antigen studies in all four and class II (HLA-DR) in three families were performed. These studies failed to show close linkage to the chromosome 6 markers in two families but there was consistent inheritance in the other two. Although formal linkage calculations are not presented, it is apparent that in some families HLA haplotyping is not useful in predicting prevence of renal obstruction.

Child↗

PubMed1994

[The central registry of bone marrow donors and the program for bone marrow transplantation from unrelated donors--20 months' experience].

The authors describe their 20 months' experience with the establishment of a bone marrow register in the Czech Republic and its practical association with the programme of bone marrow transplantations from non-related subjects. During the 20 months of activity 5455 voluntary bone marrow donors were examined and at present more than 500 new voluntary donors are examined every month. For 21 patients a HLA identical unrelated donor is sought. For four patients a HLA compatible donor was found. The first bone marrow transplantation from a unrelated donor was performed and another patient is prepared for transplantation within the next few days. The authors discuss professional, organizational, moral and ethical problems associated with the elaboration of this medical programme.

Adult↗

PubMed1993

[HLA antigens in patients with celiac disease].

In 104 non-related children with coeliac disease, all from northern Moravia, the authors assessed the distribution of HLA antigens class I and II and compared the findings with a control group. They revealed a positive association between HLA A1 (62% sick subjects as compared 27% healthy ones), B8 (68% as compared with 18%), DR3 (66% as compared with 14%) and DQw2 (79% as compared with 23%). In HLA antigens class II there is a higher relative risk of the disease for subjects with antigens DR3 and DQw2 and a higher aetiological fraction for the investigated antigens.

Adolescent↗

PubMed1992

Quantitative indices in paternity cases.

The study discusses the basic quantitative indices used as a standard method in foreign professional literature dealing with paternity cases. They are as follows: 1. mean probability of exclusion (PE) which characterizes the informative value of the experts opinions and is the same in all the disputes evaluated by this expert. 2. relative frequency of men chosen at random from the population and excluded at given phenotype of mother and child (RME). 3. probability of paternity (PP) for particular trio: mother-child-the accused man. Hereinafter the results of our studies in the HLA laboratory in Olomouc from 1976-1991 are introduced.

Blood Group Antigens↗

PubMed1992

HLA antibodies in renal transplant candidates.

The level of anti-HLA antibodies was followed in 155 patients of the chronic dialyzed program: against antigens HLA Class I and antigens HLA-DR representing HLA Class II. Correlation between the titre of these antibodies and additional factors were analyzed. The effect of pregnancy and consequently of sex was proved. Of significance were the number of dialyses undergone and the time of inclusion into the dialyses program. Positive correlation between the titre of both types of anti-HLA antibodies is so significant that it makes possible to concentrate on the more simple anti HLA I antibodies determination. The result of crossmatch test is of crucial importance when making decisions on suitable recipient of the kidney from the set of patients of dialyzed program (DP). The presence of anti-HLA alloantibodies can cause hyperacute rejection. The anti-HLA alloantibodies titre is regularly followed in DP patients. Several significant hypotheses can be verified in a large enough set of patients (hypotheses marked with ordinal numbers 1-5): 1. Only the titre of anti-HLA Class I antibodies is usually followed in DP patients. Some workplaces abroad consider it useful to follow even the anti-HLA Class II antibodies. Is anti-HLA I and HLA II antibodies titre in such a significant correlation that it is sufficient to follow only anti-HLA Class I? 2. Mechanisms of HLA antigen sensitization in DP patients are well known. We can verify the influence of transfusion number, allograft rejections and number of previous pregnancies on the anti-HLA antibodies titre.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1992

HLA-A and HLA-B typing: classification of errors.

The authors followed the frequency of errors in serologic identification of HLA-A and HLA-B antigens. They analyzed 205 paternity cases evaluated independently by two judicial experts (a revision of expert opinions). The specification of revealed discrepancies is demonstrated in the study and possible causes of their origin are analyzed. In the conclusions the authors recommend useful steps leading to the decrease of error frequency in HLA antigen typing. The HLA system--the major histocompatibility system of a man, has a broad utilization in the field of practical medicine. HLA compatibility should be respected in the choice of the couple donor-recipient for transplant therapy. HLA, as a highly polymorphic system, is successfully applied in forensic medicine. A strong association with some diseases makes it possible to utilize HLA as a supporting diagnostic sign. The commission for nomenclature of WHO at XI. International Histocompatibility Workshop (1991) recognized the following numbers of HLA specificities: 25 A, 55 B, 10 C, 21 DR, 9 PQ, 6 DP. At present HLA laboratories used mostly serological methods. The need to mutually differentiate HLA antigens which are similar due to their molecular structure puts an extraordinary demand on HLA laboratories. On one hand, a great number of HLA phenotypes exist in the population, which ensures the identification of every individual and the distinguishing of self-nonself components. On the other hand there appear the limited possibilities of HLA laboratories with relatively low numbers of diagnostic sera. We inquired how often errors occurred in HLA antigens determination. We chose a set of 205 auditing experts opinions of paternity cases for analyzing the problem.(ABSTRACT TRUNCATED AT 250 WORDS)

Child↗

PubMed1992