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Biomedical subjects

A Böhler

Publications and source records attributed to A Böhler.

9 recordsLinked to original sources
PubMed1999

Micro-column affinity test and gel test: comparative study of two techniques for red cell antibody screening.

BACKGROUND AND OBJECTIVES: We describe the results of a comparative evaluation of a gel test (ID Micro Typing) and a micro-column affinity test (MCAT, Cellbind Screen) for red cell antibody screening and identification under routine conditions. MATERIALS AND METHODS: 3,000 serum samples of patients from the Mannheim University Hospital were tested in parallel by means of the gel test and the MCAT, using the low-ionic-strength-saline indirect antiglobulin test and the protein G affinity technique, respectively. Test cells used were the same in all tests. In addition, we performed titration studies with all detected antibodies as well as with 59 frozen sera containing antibodies of known specificity. RESULTS: A total of 154 antibodies (5.1%) were detected, 149 by gel test and 147 by MCAT. The overall sensitivity and specificity of the gel test was 96.8 and 96.5% and of the MCAT 95.5 and 97.2%. No significant differences between the gel test and MCAT were found when the titer scores of all 213 (fresh and frozen) antibodies were used to check the results. The mean scores for the gel test and the MCAT were 26.8 and 28.5, respectively. For anti-Fy(a) and anti-Kell, a significantly higher titration score could be obtained in the MCAT, whereas anti-Lu(a) showed a significantly higher score with the gel test. CONCLUSION: For the screening of unexpected red blood cell antibodies, the MCAT is as sensitive as the gel indirect antiglobulin test. The sensitivity and specificity of the two systems are more or less the same although it seems that IgM antibodies are better detected by the gel test.

Antibodies, Blocking↗
PubMed1995

[Isolated lung transplantation--evaluation of patients and initial results].

Between November 1992 and May 1994 we performed 10 single and 5 double lung transplants in patients with end-stage lung diseases due to lymphangioleiomyomatosis (4), cystic fibrosis (3), pulmonary hypertension (3), pulmonary fibrosis (3) and chronic obstructive lung disease (2). In the 13 patients (87%) surviving for median 245 (19-567) days, FEV1 improved from median 640 ml to 1410 ml and the 12-minute walk distance from median 315 to 1100 meters. 10 patients (77%) enjoy a good or even excellent quality of life. 2 patients died 11 and 62 days postoperatively, due to multi-organ failure and invasive pulmonary aspergillosis respectively. The main postoperative problems are fungal and cytomegalovirus infections and chronic rejection in the form of bronchiolitis obliterans. In Switzerland as elsewhere, lung transplantation has become an established modality for the management of end-stage diseases of the lung and pulmonary circulation.

Adolescent↗
PubMed1994

[Hemoptysis].

We report on a patient with hemoptysis and discuss the differential diagnosis and the relevant diagnostic steps. The importance of the patient's history and of the clinical findings, as well as the value of the radiological and endoscopic workup, are emphasized.

Aged↗
PubMed1994

[Alcoholic pellagra encephalopathy: an underestimated treatable entity].

Alcoholic pellagra-encephalopathy is an underestimated entity, which is characterized by alteration in the level and content of consciousness, marked oppositional hypertonus and myoclonus. This entity should be included in the differential diagnosis of encephalopathy in ethanol abusers. The spontaneous course is potentially lethal. Therapy consists of substitution of nicotinic acid in the form of nicotinamide. It is emphasized that any chronic ethanol abuser with neurological symptoms should receive substitution of all B-group vitamins including nicotinamide.

Alcoholism↗
PubMed1989

[Surgical stabilization of femoral fracture following implantation of straight shaft prostheses].

Fractures of the shaft of the femur at the level of a hip endoprosthesis may cause serious problems in regard to operative stabilization as well as to bony union. The therapeutic procedure and the clinical course are described in two patients. The technical procedure is demonstrated on a plastic femur model. The advantages of cementless implantation of the femoral shaft are discussed in view of this complication.

Aged↗
PubMed1986

[Experiences with the inferior rectus abdominis flap].

Cadaveric anatomical preparations of the deep inferior epigastric artery showed the origin, the course, and the size of the artery to be consistent with few variations. Flaps which have been dissected on this vascular system can be used as island flaps or free flaps. The indications, application, and technical details, especially with regards to closure of the abdominal wall, are discussed in nine cases. The good results achieved by the use of this flap, particularly in long and narrow defects, serve as a recommendation for this type of management.

Adolescent↗
PubMedDate not supplied

Amphotericin B refractory aspergillosis after itraconazole: evidence for significant antagonism.

Failure of amphotericin B to halt aspergillosis in a patient previously treated with itraconazole raised the question of whether amphotericin B lost its activity as a result of antagonism between the two antifungal drugs. Azoles exhaust the target of amphotericin B on the fungal cell membrane. Accordingly amphotericin B in vitro lost its activity against six isolates of Aspergillus fumigatus after exposure to subfungicidal concentrations of itraconazole. Prior treatment of mice with itraconazole abolished the protective effect of amphotericin B, even when itraconazole was stopped before starting amphotericin B therapy. These observations raise concern about sequential therapy of aspergillosis with itraconazole followed by amphotericin B.

Amphotericin B↗