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A Bürgin-Wolff

Publications and source records attributed to A Bürgin-Wolff.

35 records · Page 2Linked to original sources
PubMed1980

[The state of measles immunity and the success quota of measles vaccination in Basle nursery and elementary school pupils].

Measles antibody determinations in kindergarten and primary school children in Basle-City showed that today just under 10% of 10-year-olds and roughly 25% of 5-year-olds have no immunity to measles. In the 10-year-old primary school children the effects of measles immunization are not yet markedly apparent, whereas almost half the kindergarten children already owe their measles immunity to immunization. No measles antibodies could be detected in 13,7% of the subjects who, according to their official vaccination certificates, had previously been immunized against measles. Most of these failures are probably due to careless routine immunization techniques.

Antibodies, Viral↗
PubMed1979

Antibodies to gliadin as a screening test for coeliac disease. A prospective study.

The diagnostic value of gliadin antibody determination using the fluorescent immunosorbent test was examined in a prospective study of 57 children with gastrointestinal disease. Antibodies to gliadin were found in all 20 patients with active coeliac disease, whereas 7 of these children (37%) had a normal xylose absorption test despite a flat small gut mucosa. Only 4 (14%) of 28 children with other gastrointestinal conditions had antibodies to gliadin, invariably in low titre. After at least 2 years on a gluten-free diet none of 9 children with coeliac disease in remission had demonstrable gliadin antibodies. The gliadin antibodies disappear slowly, within 6 to 24 months, after withdrawal of gliadin from the diet. 0.8% (5/606) of a healthy control group of children, adolescents and adults (not biopsied) had gliadin antibodies in low titre. Increased mean cow's milk antibody titres were demonstrable in 8 (40%) of 20 patients with active coeliac disease as well as in 9 (32%) of 28 patients with other gastrointestinal lesions. Our studies show that determination of circulating gliadin antibodies is a worthwhile screening test in suspected cases of coeliac disease. In patients so selected there is a definite indication for small intestinal biopsy to confirm the diagnosis.

Adolescent↗
PubMed1978

A/New Jersey/76 influenza vaccine trial in seronegative schoolchildren: comparison of a subunit vaccine with a whole-virus vaccine.

In the present vaccination trial, 202 seronegative schoolchildren comprising both sexes and aged 11 to 12 years were vaccinated i.m. in the upper arm with either the subunit vaccine at a dosage of 600 CCA or 200 CCA or with a whole-virus vaccine at a dosage of 200 CCA, using the double-blind procedure. Both vaccines were prepared from the strain A/New Jersey/76 (x 53a-recombinant). The vaccination was followed four weeks later by a booster injection. In tests of local and systemic reactogenicity, it was found that at both dosages the subunit vaccine caused a low frequency of minor adverse reactions. The whole-virus vaccine was marked by a significantly higher rate of adverse reactions, whether of the local or systemic variety. The whole-virus vaccine had, however, a higher immunogenicity than the subunit vaccine, and due to the relatively high rate of adverse reactions it causes, it is not recommended for the vaccination of seronegative children. Because of its low reactogenicity, the subunit vaccine can be given at higher dosage, and it is a matter for consideration whether a better antibody response might not result from two booster injections.

Antibodies, Viral↗
PubMed1976

Screening for mumps immunity with the microtiter solid-phase radioimmunoassay.

A solid phase radioimmunoassay on microtiter plates was adapted for the estimation of mumps antibodies using commercially available complement-fixing mumps antigen. The sensitivity of the test is superior to that of hemagglutination inhibition and lies in the same range as the virus neutralization test performed on chick fibroblasts. The method is useful for screening large series of human sera, for instance in connection with vaccination programs.

Antibodies, Viral↗
PubMed1976

Immunofluorescent antibodies against gliadin: a screening test for coeliac disease.

A sensitive and simple immunofluorescence test for gliadin antibodies is described, which may be suitable as a screening method for coeliac disease especially in cases with no clear-cut indication for performing a biopsy. In serum samples of 19 from 23 patients (83%) with coeliac disease proved by biopsy antibodies against gliadin could be detected. In a control group of 71 children in whom the clinical diagnosis of another malabsorptive disorder was made gliadin-IF-antibodies were found in only 3 children (4%). With better timing of blood sampling (first sample during a period with gluten containing food and in case of a negative result a second sample after 4-8 days of gluten withdrawal) the sensitivity of the screening test will be improved. The described gliadin-IF-test permits the classification of the antibodies into the different immunoglobulin classes. In each child with a positive result IgG antibodies against gliadin were detected. In 9 respectively 4 of the 19 patients with IgG antibodies IgA- respectively IgM-antibodies against gliadin were also found. Antibodies against at least one cow milk protein were identified in nearly all (91%) coeliac patients and in half (49%) of the patients with other malabsorptive disorders.

Antibodies↗
PubMed1971

Separation of rubella IgM, IgA, and IgG antibodies by gel filtration on agarose.

Identification of the immunoglobulin classes of rubella antibodies is the only reliable tool for the diagnosis of a recent primary infection with rubella within 6-8 weeks of infection. Gel filtration permits the separation of IgM and IgA antibodies, both characteristic for a recent infection, from persistent rubella IgG antibody (which indicates pre-existing immunity). Gel filtration on agarose offers advantages over the other methods for separating immunoglobulin classes. Fractionation of specific rubella antibodies is important not only in all cases where a serum sample was taken too late and a rise of antibody titre missed, but also in cases with significant antibody rise to distinguish a secondary immune response after rubella reinfection.

Antibodies, Viral↗
PubMedDate not supplied

[Experiments with simultaneous immunization against cytomegalovirus infection and rubella].

An immunisation trial with simultaneously applicated attenuated rubellavirus (Cendehill) and attenuated cytomegalovirus (TOWNE 125) was done in adults. There was no difference in antibody-titers against both viruses and in side-reactions (local ones only) between simultaneous or single application of the two viruses. Adults immunized against cytomegalovirus two years ago still had IF-IgG-antibodies; in only 1 of 8 volunteers there was a significant antibody-decline.

Antibodies, Viral↗
PubMedDate not supplied

[Quality assurance in diagnostics: are there normal values for IgG-antibodies to cow's milk proteins?].

BACKGROUND: The value of IgG-cow's milk antibodies is heavily disputed. Therefore, aim of the study was to establish normal values for these IgG-antibodies and to determine their diagnostic significance especially for gastrointestinal allergic manifestations. PATIENTS AND METHODS: 702 newborns were included prospectively. They received different feedings: mothers milk, cow's milk based formula and a partially or an extensively hydrolyzed formula. All children were examined annually or on occasion of allergic manifestations. An indirect immunofluorescent test was used for the assessment of IgG-antibodies against casein, beta-lactoglobulin, alpha-lactalbumin and bovine serum albumin. Values were expressed as geometric mean titers. RESULTS: IgG-antibody titers rose slowly in the exclusively breast-fed children and peaked rapidly in the cow's milk formula-fed group, especially during the first year of life. There were no significant differences between children with and without allergic manifestations. Because of a large overlap between asymptomatic and symptomatic children it was impossible to establish normal values for the IgG-cow's milk antibodies. CONCLUSIONS: The occurrence of IgG-cow's milk antibodies is a physiologic phenomenon without diagnostic significance. The costs of these determinations can be saved to improve process quality.

Animals↗