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Biomedical subjects

A Bagrel

Publications and source records attributed to A Bagrel.

6 recordsLinked to original sources
PubMed1979

Relations between reported alcohol consumption and certain biological variables in an "unselected" population.

We looked for variables that could serve as indexes of alcohol consumption. We tested iron, gamma-glutamyltransferase, mean erythrocyte volume, mean erythrocyte hemoglobin concentration, urea, uric acid, and alanine aminotransferase, among others, in an unselected population of men, and by principal components analysis we singled out the correlation between the two enzymes and the two hematologic values. On the other hand, calculation of the coefficients of correlation between the total amount of alcohol consumed and the 10 variables studied, together with multiple regression analysis, showed that the three variables that correlated most significantly with alcohol consumption were gamma-glutamyltransferase, mean erythrocyte volume, and the use of tobacco. Critical evaluation of the results leads us to conclude that still more discriminative biological indices msut be sought and that the use of psychosocial data is also desirable.

Adult↗
PubMed1978

[Enzyme multiplied immunoassay technic (EMIT) (author's transl)].

In 1960, Yalow and Berson opened the route to radioimmunoassay. Now the technic of radioimmunoassay requires apart from the use of artificial radioisotopes, a compulsory stage of separation of free and linked phases. The author has developed a technic in homogeneous phase "EMIT" which avoids the intermediate stage of separation. He gives here the principle, studies the various parameters of the method, shows the advantages and disadvantages and compares it with other technics and finally mentions future prospects.

Antigen-Antibody Reactions↗
PubMed1976

Ornithine carbamoyl transferase activity. Use of a bovine liver extract in quality control.

To obtain a pure ornithine carbamoyltransferase for quality control in clinical chemistry, the authors compared human plasma and bovine liver ornithine carbamoyltransferase. The data show that the KM of both enzymes are similar (respectively 1.03 and 1.33 mmol when ornithine is used as substrate; 1.02 and 1.06 mmol with carbamyl phosphate). The following properties are shown to be equivalent: linearity of the enzymatic reaction, inhibition by higher substrate concentrations, and temperature denaturation. The similarities between the two enzymes indicate that bovine liver ornithine carbamoyltransferase can be used in clinical chemistry quality control.

Animals↗
PubMed1975

Colorimetric measurement of ornithine carbamoyl transferase activity in plasma, and results for a supposedly healthy population.

Determination of ornithine carbamoyl transferase (EC 2.1.3.3) activity in plasma is important for detection of liver diseases. The assay established in this paper has been made optimum. A blank is needed containing both substrates, carbamoyl phosphate and ornithine. We used a new colorimetric assay, based on a complex with a phosphoferric-antipyrine reagent and diacetyl monoxime, to measure the citrulline formed. The highly sensitive assay permits low activities to be determined accurately. Values for blood plasma from 425 supposedly healthy people, varied from 0 to 16 U/liter (95th percentile), and 27% of this population showed an activity of less than 2 mumol of citrulline formed per minute per liter, 2 U/liter being the limit of the method's sensitivity.

Colorimetry↗