PubMed Health⌕ Search

Biomedical subjects

A Bano

Publications and source records attributed to A Bano.

At least 19 recordsLinked to original sources
PubMed2001

Isolation and 16S rRNA sequence analysis of the beneficial bacteria from the rhizosphere of rice.

The present study deals with the isolation of plant growth promoting rhizobacteria (PGPR) from rice (variety NIAB IRRI-9) and the beneficial effects of these inoculants on two Basmati rice varieties. Nitrogen-fixing activity (acetylene-reduction activity) was detected in the roots and submerged shoots of field-grown rice variety NIAB IRRI-9. Estimation of the population size of diazotrophic bacteria by ARA-based MPN (acetylene reduction assay-based most probable number) in roots and shoots indicated about 10(5)-10(6) counts/g dry weight at panicle initiation and grain filling stages. Four bacterial isolates from rice roots and shoots were obtained in pure culture which produced phytohormone indoleacetic acid (IAA) in the growth medium. Among these, three isolates S1, S4, and R3 reduced acetylene to ethylene in nitrogen-free semi-solid medium. Morphological and physiological characteristics of the isolates indicated that three nitrogen-fixing isolates S1, S4, and R3 belonged to the genus Enterobacter, while the non-fixing isolate R8 belonged to the genus Aeromonas. 16S rRNA sequence of one isolate from root (R8) and one isolate from shoot (S1) was obtained which confirmed identification of the isolates as Aeromonas veronii and Enterobacter cloacae, respectively. The 1517-nucleotide-long sequence of the isolate R8 showed 99% similarity with Aeromonas veronii (accession No. AF099023) while partial 16S rRNA sequence (two stretches of total 1271 nucleotide length) of S1 showed 97% similarity with the sequence of Enterobacter cloacae (accession No. AJ251469). The seedlings of two rice varieties Basmati 385 and Super Basmati were inoculated with the four bacterial isolates from rice and one Azospirillum brasilense strain Wb3, which was isolated from wheat. In the rice variety Basmati 385, maximum increase in root area and plant biomass was obtained in plants inoculated with Enterobacter S1 and Azospirillum Wb3, whereas in the rice variety Super Basmati, inoculation with Enterobacter R3 resulted in maximum increase of root area and plant biomass. Nitrogen fixation was quantified by using 15N isotopic dilution method. Maximum fixation was observed in Basmati 385 with the inoculants Azospirillum Wb3 and Enterobacter S1 where nearly 46% and 41% of the nitrogen was derived from atmosphere (%Ndfa), respectively. In general, higher nitrogen fixation was observed in variety Basmati 385 than in Super Basmati, and different bacterial strains were found more effective as inoculants for the rice varieties Basmati 385 and Super Basmati.

Aeromonas↗
PubMed1998

Pulmonary vagal innervation is required to establish adequate alveolar ventilation in the newborn lamb.

To investigate the effects of bilateral intrathoracic vagotomy on the establishment of continuous breathing and effective gas exchange at birth, we studied 8 chronically instrumented, unanesthetized, sham-operated and 14 vagotomized newborn lambs after a spontaneous, unassisted vaginal delivery. Fetal lambs were instrumented in utero to record sleep states, diaphragmatic electromyogram, blood pressure, arterial pH, and blood-gas tensions. Six of eight sham-operated lambs established effective gas exchange within 10 min of birth, whereas 12 of 14 vagotomized animals developed respiratory acidosis and hypoxemia (P = 0.008). Breathing frequency in vagotomized newborns was significantly lower during the entire postnatal period compared with sham-operated newborns. Vagotomized subjects also remained hypothermic during the entire postnatal period (P < 0.05). Bronchoalveolar lavage indicated an increased minimum surface tension, whereas lung histology showed perivascular edema and partial atelectasis in the vagotomized group. We conclude that stimulation of breathing and effective gas exchange are critically dependent on intact vagal nerves during the transition from fetal to neonatal life.

Animals↗
PubMed1996

Umbilical arterial blood flow and plasma prostaglandin E2 concentrations during arousal and breathing movements in fetal sheep.

To investigate the effects of lung distension and oxygenation on umbilical blood flow (UBF) and plasma prostaglandin E2 (PGE2) in relation to arousal and stimulation of breathing movements, we studied eight chronically instrumented, unanesthetized fetal sheep between 137 and 143 d of gestation. Electrocorticogram, electro-oculogram, nuchal and diaphragmatic electromyograms, arterial pH and blood gas tensions, Hb oxygen saturation, body temperature, and UBF were recorded in each fetus. Electrocorticogram, electro-oculogram, and nuchal electromyograms were used to define sleep states. No sooner than 4 d after surgery, fetal lungs were distended with 100% O2 or N2 in a randomized order via an in situ Y-endotracheal tube. PGE2 concentrations were analyzed by RIA. A significant increase in fetal arousal and stimulation of breathing during nonrapid eye movement sleep was observed during lung distension with O2 as compared with control periods and lung distension with nitrogen. In all sleep states, UBF significantly decreased during oxygenation as compared with the control values. However, no significant correlation was observed between the time of the onset of arousal and the decrease in UBF. Lung distension with N2 resulted in increased plasma PGE2 concentrations, whereas, no change was observed during oxygenation. Our data suggest that an increase in fetal partial pressure of arterial O2 leads to a decrease in UBF. However, the onset of arousal and stimulation of breathing during lung distension and oxygenation were not dependent on a decrease in plasma PGE2 concentrations.

Animals↗
PubMed1988

[Small size breast cancer conservative surgical therapy and subsequent radiotherapy].

From february 1980 to december 1985, 115 breast carcinomas were treated with QU.A.RT. with limited surgery and following radiotherapy. Median age was 51 years (range 25 to 75). Surgical approach was quadrantectomy with axillary nodes dissection. Irradiation of residual breast was performed by an anterior high energy electron beam. 50 Gy in 25 fractions was the minimal dose to whole breast. In pN+ patients systemic adjuvant therapy was done. Local control was obtained in 111 cases. Local recurrence appeared in 4: 1 menopausal pT1 pNO and 3 premenopausal (1 pT1 pNO and 2 pT1 pN+) at respectively 31, 19, 22 months from diagnosis. Distant metastases appeared in 10 patients. 7 died for cancer and 3 are still alive. Cosmetic results were scored as good in 52, better than mastectomy in 26, unsatisfactory in 3, and not stated in 34. Our results are comparable to those of other Centers. Youth, axillary metastases and delay between surgery and radiotherapy appears to influence the risk of recurrence also in our series.

Adult↗
PubMed1988

[Primary carcinoma of the gallbladder: ultrasonic features (remarks on 8 cases)].

The authors illustrate the ultrasound features of gallbladder carcinoma after reviewing literature and basing upon their own experience. The diagnosis of this neoplasia is formulated more frequently today by introduction of scanning means, but it remains very difficult on initial phase because of its precocious lymphatic and hilar infiltration.

Aged↗
PubMed1987

[Hepatic angioma: a bothersome presence during staging of neoplasms of the digestive system].

On reviewing a consecutive series of 1,200 ultrasonic tomographies of the liver to assess the incidence of liver angiomas, the authors describe their sonographic, tomodensitometric and angiographic characteristics. They emphasise the fact that such lesions, though usually not constituting a serious clinical or diagnostic problem, as a simple sonographic follow-up will suffice to clarify the situation, may nevertheless, in certain particular clinical situations such as in the course of tumour staging of the digestive apparatus, require full diagnostic investigation using all the techniques available (ultrasonic tomography, CAT, liver arteriography, sonographically guided thin-needle biopsy.

Adult↗
PubMed1987

[Leiomyosarcoma of the duodenum (description of a case and review of the literature)].

The Authors report on a case of duodenal leiomyosarcoma of small dimensions in a paravaterian site and presenting a low degree of histological malignity. They summarize the main anatomoclinical features of the neoplasm and conclude by indicating less aggressive surgical therapy as adequate in such cases, accompanied, however, by a protracted post-operative follow-up.

Duodenal Neoplasms↗
PubMed1986

["Wandering spleen": preoperative diagnosis by echotomography of the abdomen (review of the literature and report of a case)].

Splenic wandering is a rare condition of upset position of spleen, due to congenital fault or to excessive looseness of the supporting apparatus of this organ. The literature reports about 180 cases thereof, few of which recognized as such before operation. Ultimate investigations, in this limited number of preoperative diagnoses, resulted angiography and hepato-splenic scintigraphy; only three times the diagnosis was correctly placed through ultra-sounds. The authors show a case, confirmed by the operative report, where the ultrasonic test allowed to place the diagnosis with certainty, and, due to a particular lot of circumstances, proved to be better than TAC.

Adult↗
PubMed1986

[Clinico-diagnostic aspects of aggressive angiomyxoma of the pelvis and perineum].

The authors report on a case of aggressive angiomyxoma of the pelvis and perineum in a 46-year-old female patient. After reviewing the cases described in the literature, they examine the clinical characteristics of this rare form of neoplasia, which tends to occur more frequently in patients of female sex and in the young to middle-aged age bracket and is characterized by slow growth, non-infiltration of adjacent organs and a tendency to recur owing to incomplete excision. Metastases have never been reported. Failure to recognize the tumor at first approach may be due to the fact that it is easy to confuse it clinically with other more common diseases of the perineum. The authors conclude by advising systematic anatomicopathological identification of all perineal tumors, even when apparently benign.

Female↗
PubMed1986

[Epidemiology and monitoring of closed abdominal injuries].

The authors review the epidemiological and aetiopathogenetic characteristics of abdominal blunt injuries (118 cases) monitored in a homogeneous manner. The relatively young age of the patients, the diagnostic difficulties encountered and the frequent presence of associated lesions call for the rigorous application of a predetermined clinico-diagnostic protocol.

Abdominal Injuries↗
PubMed1986

[Ductal adenoma of the breast: correlations between clinico-radiologic and histo-pathologic aspects (considerations on a case)].

The authors report on a case of ductal adenoma of the breast, pointing out that routine clinico-instrumental diagnostic signs, though suggesting a picture of malignancy, may actually relate to this rare benign form of tumor. In the presence of cases of this type, we therefore advise a cautions approach to surgery, in that oncological radicality is subordinate to confirmation in the form of a traditional histological preparation, as freezer examinations are unable to provide wholly reliable responses.

Adult↗
PubMed1985

[Diagnostic up-date in thrombosis of the portal vein].

Portal thrombosis is a rare acquired condition with various etiology. Clinical manifestations are portal hypertension or nine. The main technics to diagnostic are: CT, indirect splenoportography and ultrasound examination. The first and the second are reliable but the third only is useful to explore the portal system and to evaluate patients with suspect portal vein thrombosis.

Aged↗
PubMed1978

[Cancer of the gastric stump (author's transl)].

On the evidence of 24 cases of cancer of the gastric stump the authors discuss the real incidence of this dread complication; they conclude that starting 8 years after primary surgery, all patients carrying a gastric stump must be regarded as high-risk subjects for malignancy; further, they recommend that pyloro-gastrectomy after Billroth (II) be considered for execution in young patients with all possible reluctance.

Gastrectomy↗