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Biomedical subjects

A Basomba

Publications and source records attributed to A Basomba.

At least 37 records · Page 2Linked to original sources
PubMed1982

Detection of Aspergillus antigen and anti-Aspergillus precipitating antibodies by a new method: the radial immunoelectro-osmophoresis (RIEOP).

A new method of Immunoprecipitation-in-gel, the Radial Immunoelectro-osmophoresis (RIEOP) has been applied to detect precipitating anti-Aspergillus fumigatus antibodies and to study the antigens present in various antigenic extracts of this fungus. The results obtained with this method have been compared with those obtained when applying the Comparative Double Diffusion (CDD) and the Immunoelectro-osmophoresis (IEOP). The data presented prove that the RIEOP method is more sensitive than CDD and the IEOP in: (1) detecting precipitating anti-Aspergillus antibodies, since (a) precipitating bands are detected with higher serum dilutions, and (b) when using undiluted serum, the number of precipitating bands obtained by RIEOP is higher; and (2) studying antigens present in the antigenic extracts, since (a) the number of antigens detected by RIEOP is higher than that by CDD, and (b) identity/non-identity reactions can be studied among the antigens present in the various extracts.

Antibodies, Fungal↗
PubMed1979

IgE antibodies against penicillin as determined by Phadebas RAST.

Serum samples from eighty-one patients with suspected penicillin allergy were investigated with Phadebas RAST using the penicillin derivatives Benzylpenicilloyl-human serum albumin (PBO-HSA) and Phenoxymethylpenicilloyl-human serum albumin (PMPO-HSA) and the results were compared with skin test results and clinical data. Of the sixty-one patients who had anaphylactic shock and/or urticaria as a possible consequence of penicillin administration, reagins against PBO-HAS and PMPO-HSA could be detected in thirty-four cases (56%). Five per cent of these patients, with positive RAST results, showed negative skin tests; in the other 95% both RAST and skin tests were positive. All, except eight, of the RAST-negative patients had had their adverse reactions at least 2 years prior to the blood sampling and in some of these cases skin tests were also negative. RAST and provocation test results agreed in 80% of the cases where exposition was performed. It is concluded that the RAST technique is a valuable diagnostic tool for the detection of immediate type hypersensitivity to penicillin.

Anaphylaxis↗
PubMed1978

Allergy to penicillin unsuccessfully treated with a haptenic inhibitor (benzyl-penicilloyl-N2-formil-lysine; BPO-flys). A case report.

In a patient with a past history of allergy to penicillin and requiring treatment, skin tests with penicillin and penicilloyl-polylysine (PPL) were positive, and a penicilloyl RAST was strongly positive, although the Prausnitz-Küstner test to penicillin and PPL were negative. The haptin BPO-Flys was administered together with penicillin to try to prevent reactions. An anaphylactic reaction occurred on the fifth day, and treatment was stopped. Serum total IgE values increased markedly after the reaction and PK titres to penicillin and PPL reached values of 1/256. The skin test reaction to PPL was negative the day after the clinical reaction, but became strongly positive again a few days later. The penicilloyl RAST remained strongly positive throughout. The authors consider that there was hypersensitivity to a penicillin metabolite other than the penicilloyl group, e.g. to minor determinants, and for this reason the hapten inhibitor failed.

Aged↗
PubMed1976

The effect of sodium cromoglycate in preventing aspirin induced bronchospasm.

Seventeen patients with aspirin-induced asthma were studied, the majority being intolerant to more than one analgesic. In addition to asthma, eleven patients had sinusitis and eight had nasal polyps. Serum IgE levels were normal with a mean of 295 iu/ml. However, some patients had positive cutaneous and PK tests against inhalants and non-analgesic drugs. Spirometry showed the bronchial obstruction to be mild. However, all patients were hyper-reactive to acetylcholine. Oral provocation tests with aspirin alone and also with the prior administration of sodium cromoglycate (SCG) by inhalation were performed and the results assessed by spirometry and clinical examination. The results suggest that the obstruction is probably due to oedema of the bronchial mucosa together with pulmonary congestion rather than a simple spasm of the bronchi. SCG was found to prevent significantly the ventilatory obstruction induced by aspirin. It is suggested that non-immunological factors are responsible for the asthma and that SCG may have an effect on the altered receptors protecting them from the action of aspirin on kinins.

Adult↗
PubMedDate not supplied

Immunotherapy with a mass unit Parietaria judaica extract: a tolerance study with evidence of immunological changes to the major allergen Par j 1.

Specific immunotherapy with Parietaria judaica pollen extract has been proven to be effective in the treatment of patients with respiratory allergy induced by this pollen. Nevertheless, there is some controversy about its tolerability. We conducted an open uncontrolled study to evaluate the tolerability of an aluminium adsorbed P. judaica pollen extract whose major allergen (Par j 1) content was known. Changes in immunological parameters to a complete P. judaica extract and to a purified Par j 1 preparation were monitored. Twenty-one patients (12 women, 9 men; mean age 30.2 years) suffering from rhinoconjunctivitis and/or asthma due to Parietaria pollen were enrolled. The maximum dose was established at 10 BU/ml (0.6 microgram Par j 1). Skin and conjunctival reactivity as well as serum levels of specific IgE, IgG, IgG1 and IgG4 were evaluated before therapy (T0), when 1 BU was given (T1), 2 weeks after the maintenance dose was reached (T2) and after the pollen season (T3). Four-hundred and fifteen doses were administered during immunotherapy. Only one systemic reaction (0.24% of doses) and two local reactions were registered. Reactions occurred during the administration of the highest concentrated vial. Before immunotherapy, purified Par j 1 accounted for 94.2% of the cutaneous response elicited by the complete extract. A statistically significant decrease in cutaneous response was detected after 8 weeks of treatment. There were no significant changes in conjunctival reactivity throughout the study. Specific IgG, IgG1 and IgG4 showed a pronounced and significant increase during the study, while specific IgE levels initially decreased and increased after the pollen season. The kinetics of specific antibodies to P. judaica complete extract and purified Par j 1 showed a parallel trend. The present study demonstrates that immunotherapy with P. judaica extract is well tolerated in patients suffering from rhinoconjunctivitis and/or asthma due to Parietaria pollen. This therapy induces specific changes in the immunological response to P. judaica and to purified Par j 1. These changes can be detected at very early stages of therapy.

Adolescent↗
PubMedDate not supplied

Occupational asthma induced by chromium salts.

Five patients with asthma related to exposure to chromium salts, in their work area, are presented. All of them were non atopics and presented a history of contact dermatitis, with positive patch tests to potassium dichromate, previous to the onset of bronchial asthma. Solutions of K2Cr2O5 were prepared in normal saline at 0.01, 0.1 and 1 mg/ml for skin prick tests (SPT) and bronchial provocation tests (BPT). Immediate cutaneous reaction by SPT was negative for controls and patients. BPT were performed by the tidal breathing method, with positive results in all subjects. A negative response was recorded in 4 control unexposed asthmatics. An attempt to inhibit BPT with sodium cromoglycate was unsuccessful. The diversity of reactions (immediate, dual and late) registered in BPT, support that bronchial reactivity can be induced specifically by inhalation of chromium salts. The data of follow-up indicates a good prognosis, provided that patients remain out of exposure. The lack of facts suggesting an IgE-mediated reaction puts forward for consideration other pathophysiologic mechanisms.

Adult↗
PubMedDate not supplied

Eosinophilic gastroenteritis. Three cases involving each of the anatomo-clinical variants.

Three patients suffering from eosinophilic gastroenteritis belonging to each of the three anatomo-clinical forms are involved in this study. The disease has a high blood eosinophilia and in the anatomo-pathological studies it is established that there is an eosinophilic infiltration in some portion of the gastrointestinal tract. The localization of this infiltration in the mucous, muscular or serous layers of the digestive canal allows the three varieties to be distinguished, each one having an individual clinical pattern. In the patient with the mucous form, analytical and clinical evidence of atopy is observed, as well as certain types of food which trigger off the symptoms. The exclusion diet and the membrane-stabilizing drugs, disodium cromoglycate and ketotifen produce clear therapeutic effects. Conversely, in those patients where the eosinophilic infiltration is in the muscular or serous layers, no food inductors are found and a therapy based on diet with subsequent administration of the above-mentioned drugs proves to be ineffective. The course of the disease in these cases is unpredictable and steroid drugs must be used frequently. The clinical, anatomo-pathological and analytical characteristics, together with the therapeutic results and subsequent development, lead us to assume a reaginic immunological mechanism in the mucous membrane, but, in the other forms there is no clear pathogenic mechanism to explain the anatomo-pathological findings and the clinical symptoms.

Adult↗