PubMed Health⌕ Search

Biomedical subjects

A Bensaid

Publications and source records attributed to A Bensaid.

At least 19 recordsLinked to original sources
PubMed2003

[Emphysematous pyelonephritis: a case report].

Diabetic patients with urinary tract infections had a certain risk of developping emphysematous pyelonephritis with gas producting bacteria. This disease was accompagned with high mortalité. We will report one case who was treated successfully by high dose antibiotic regim and emergency nephrectomy.

Anti-Bacterial Agents↗
PubMed2002

IFN-gamma as an indicator of successful immunization of goats vaccinated with a killed Cowdria ruminantium vaccine.

Cowdria ruminantium-induced production of IFN-gamma was measured by ELISA on a weekly basis during the course of vaccination with killed organisms emulsified in ISA50. Upon challenge, all (3/3) vaccinated animals that gave the lowest IFN-gamma response died of peracute cowdriosis. On the other hand, only one of three animals showing high IFN-gamma responses to vaccination died, but with a delay of 4 days in comparison with naïve controls. Thus, there seems to be a threshold level of IFN-gamma below which the probability for vaccinated animals to survive a lethal challenge is very low. During challenge, a much lower, but still physiologically meaningful production of IFN-gamma was detected using the 24-hour whole blood assay on day 5 after infection in animals controlling the infection. In contrast, IFN-gamma production was absent or negligible in naïve and vaccinated animals that died within 8-10 days after infection. Although these results need to be validated on a larger number of animals, they strongly suggest that IFN-gamma is a useful indicator of protective immunity in animals immunized with killed COWDRIA:

Animals↗
PubMed2002

Cowdria ruminantium antigens of around 15 kDa are potent inducers of IFN-gamma.

Cellular responses induced in two Creole goats by vaccination with killed Cowdria ruminantium (Cowdria) were confirmed by IFN-gamma production and interleukin-2 receptor (IL-2R) expression. Both CD4+ and CD8+ but not WC1+ T cells showed a substantial increase in cell surface expression of IL-2R molecules in response to whole Cowdria lysate. Cowdria (Welgevonden strain) proteins were fractionated using continuous-flow electrophoresis and tested for their ability to induce IFN-gamma production by PBMC collected three weeks after the first inoculation and one week after the booster injection. Pooled fractions of around 15, 22, and 24 kDa were found to induce significant IFN-gamma production in both vaccinated animals on one of the two occasions. Antigens of around 15 kDa induced substantially higher IFN-gamma production than any other fractions in both animals. These pilot experiments pave the way towards the identification of proteins/genes that have potential for the development of a recombinant vaccine against heartwater.

Animals↗
PubMed2001

Ehrlichia ruminantium major antigenic protein gene (map1) variants are not geographically constrained and show no evidence of having evolved under positive selection pressure.

In a search for tools to distinguish antigenic variants of Ehrlichia ruminantium, we sequenced the major antigenic protein genes (map1 genes) of 21 different isolates and found that the sequence polymorphisms were too great to permit the design of probes which could be used as markers for immunogenicity. Phylogenetic comparison of the 21 deduced MAP1 sequences plus another 9 sequences which had been previously published did not reveal any geographic clustering among the isolates. Maximum likelihood analysis of codon and amino acid changes over the phylogeny provided no statistical evidence that the gene is under positive selection pressure, suggesting that it may not be important for the evasion of host immune responses.

Amino Acid Sequence↗
PubMed2001

[Malignant non-Hodgkin's lymphoma presenting with cardiac tamponade].

Malignant non-Hodgkin's lymphoma rarely involves the heart. However, all cardiac structures may be invaded, although the myocardium and pericardium are affected more often than the valvular structures. Diagnosis is important because treatment must be adapted. The cardiac signs are usually non-specific and lymphomas presenting in this way have only occasionally been reported. The authors report two cases in which malignant non-Hodgkin's lymphoma presented with cardiac tamponade.

Adult↗
PubMed2000

The use of the tetrazolium salt XTT for the estimation of biological activity of activated sludge cultivated under steady-state and transient regimes.

The tetrazolium salt 3'-¿1-[(phenylamino)-carbonyl]-3, 4-tetrazolium¿-bis (4-methoxy-6-nitro) benzenesulfonic acid hydrate (XTT) was used as a tool for estimating the activity of the electron transport system (ETS) in activated sludge cultivated under steady-state and transient regimes in chemostat culture. Production of formazan by reduction of XTT depended on the initial concentration of the XTT following a saturation law and was proportional to live cell biomass. Addition of cyanide (KCN) to activated sludge gave an initial 1.5-fold increase in XTT reduction, while addition of 3,5-dichlorophenol (3,5-DCP) reduced this value drastically. At steady-state and transient regimes of an activated sludge chemostat, oxygen uptake rate (OUR) and XTT reduction rate were highly correlated and indicated significant variations depending on the growth conditions.

Bacteria↗
PubMed1999

Bovine CD4+ T-cells lines reactive with soluble and membrane antigens of Cowdria ruminantium.

Cowdria-specific CD4+ T-cell lines generated from immunised cattle respond to both soluble and membrane proteins of the agent. Furthermore, the lines produced the Cowdria-inhibitory cytokine IFN-gamma in response to soluble antigens fractionated by gel filtration and FPLC. Activity eluted as a single peak around fraction 15 for all T-cell lines tested. This fraction induced the highest production of IFN-gamma by the lines and was shown by SDS-polyacrylamide gel electrophoresis and silver staining analysis to contain less than 10 different bands ranging from 22 to 32 kDa. Given their high sensitivity and specificity, these short-term CD4+ T-cell lines will be valuable tools for the identification of Cowdria antigens for incorporation in a subunit vaccine.

Animals↗
PubMed1999

Effect of isolation techniques, in vitro culture and IFNgamma treatment on the constitutive expression of MHC Class I and Class II molecules on goat neutrophils.

Previous studies on the ability of human neutrophils to synthesize cytokines and express MHC Class I and inducible Class II molecules have suggested a possible role of these cells as accessory or antigen presenting cells (APC). There is no information available to date concerning this aspect in ruminants. Therefore, as the first step of these investigations, we have studied the cell surface expression of MHC molecules on goat neutrophils. We show that goat neutrophils can be distinguished from eosinophils with monoclonal antibody (MoAb) ILA-24 which recognizes cattle monocytes and neutrophils. Goat neutrophils constitutively express MHC Class II molecules. However, cell surface expression of MHC Class I and Class II molecules is dramatically reduced on neutrophils purified by density gradient centrifugation in comparison to neutrophils obtained from whole blood after lysis of erythrocytes. Also, the level of expression of MHC Class I antigens is seasonal and donor-dependent and rapidly decreases after in vitro culture despite negligible necrosis and apoptosis of neutrophils. Although treatment with IFNgamma partially prevents the loss of MHC Class I molecules on neutrophils, it fails to induce MHC Class II antigens. Implications of these results for further studies on the potential role of neutrophils as APC are discussed.

Animals↗
PubMed1999

Immune responses to Cowdria ruminantium infections.

Understanding the basis of protective immunity to Cowdria ruminantium will facilitate the development of an effective subunit vaccine against heartwater in ruminants and contribute to a better definition of protective immune mechanisms to obligate intracellular pathogens in general. Until recently, immunological studies of heartwater in ruminants concentrated solely on antibody responses. Since 1995, the mechanisms underlying cell-mediated immunity of heartwater have been analysed. Progress achieved in these areas is discussed here by Philippe Totté and colleagues, with special emphasis on ruminants, the natural hosts of C. ruminantium.

Animals↗
PubMed1999

Characterization of 18 new BoLA-DRB3 alleles.

The second exon of the bovine MHC class II DRB3 gene was amplified by polymerase chain reaction (PCR) from DNA samples of 568 zebu Brahman cattle (Bos indicus) from Martinique (French West Indies). Cloning of these PCR products allowed the isolation of both alleles from each animal, which were characterized by the PCR-restriction fragment length polymorphism (RFLP) technique using the restriction enzymes RsaI, BstYI and HaeIII. Four new PCR-RFLP patterns were obtained by digestion with RsaI. These patterns were named 'v', 'w', 'x' and 'y' continuing the accepted nomenclature. Sequencing of each allele allowed the identification of 18 new BoLA-DRB3 exon 2 nucleotide sequences and their deduced amino acid sequences.

Alleles↗
PubMed1998

Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium.

The response of PBMC collected from cattle immunized with killed Cowdria to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells proliferated in response to recombinant proteins. Short-term cell lines derived from PBMC using Cowdria lysates were more than 90% CD4+ and did not respond to any of the recombinant proteins. Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks. Only one cell line could be generated using MAP1 and it was composed of 85% gamma delta T cells. This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes.

Animals↗
PubMed1998

Inhibitory effect of Cowdria ruminantium on the expression of MHC class I and class II molecules on bovine endothelial cells.

Bovine endothelial cells constitutively express MHC class I molecules, whereas MHC class II molecules can be induced by interferon gamma (IFN gamma) treatment. Endothelial cells may play a role as antigen presenting cells (APC), but their role in the immune response against Cowdria ruminantium is unknown. We have studied the effect of C. ruminantium infection on the expression of MHC class I and class II molecules on the surface of bovine endothelial cells. Bovine brain endothelial cells (BBEC) from the microvasculature and bovine umbilical endothelial cells (BUEC) from the macrovasculature were cultured in vitro, infected with different concentration of Cowdria, and treated or not with IFN gamma. We observed that Cowdria induced a reduction of MHC molecules expression on the surface of BUEC and BBEC. This inhibitory effect of Cowdria on MHC molecules could affect the capacity of endothelial cells to act as efficient antigen-presenting cells.

Animals↗