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Biomedical subjects

A Berc

Publications and source records attributed to A Berc.

6 recordsLinked to original sources
PubMed2006

Expression of MDR proteins in breast cancer and its correlation with some clinical and pathological parameters.

The aim of this work was to determine the expression of the multidrug resistance (MDR) proteins, namely MDR1 (P-glycoprotein), MRP1 (multidrug resistance-related protein) and LRP (lung resistance-related protein), in 87 samples of breast carcinoma. Detection of these proteins was provided by using indirect enzymatic immunohistochemistry. Our findings were compared with the other clinical and pathological parameters: expression of Her2/neu, estrogen receptor status (ER), progesteron receptor status (PR), histological grade and regional lymph node status. For statistical analysis, non-parametric two sided Mann-Whitney-U test was used. Majority of breast carcinoma specimens show positivity for these proteins. The MDR1 and MRP1 signal was found in the cytoplasm of cancer cells. The expression of LRP was detected in the cytoplasm close to the nuclear membrane. The samples were positive for MDR1 protein in 57%, for MRP1 in 84% and for LRP in 79%. Comparing our results with other clinical and pathological parameters, negative correlation between ER, PR and MDR1 expressions and histological grading status was found. No associations were observed between the MRP1 and LRP proteins and histological grading, as well as between the expression of three MDR proteins and the other clinically relevant parameters. In conclusion, high frequency of expression of MDR proteins in breast carcinoma cells suggests, that these proteins might be an important factor of drug resistance in breast carcinoma. Nevertheless, the negative correlation between the histological grade of malignancy of tumor and the expression of ER, PR and MDR1 indicates possible influence of progressive tumor cell de-differentiation. However, this finding has to be confirmed in additional evaluations.

Biomarkers, Tumor↗
PubMed1999

Yersinia ruckeri septicaemia in experimentally infected carp (Cyprinus carpio L.) fingerlings.

The presence of Yersinia ruckeri, the causal agent of enteric redmouth disease (ERM) in salmonids and a few other freshwater fish, has so far been reported from a variety of sources including the intestine of healthy carp. Since there are no data on the pathogenicity of this bacterium for carp, 15 fingerlings were experimentally infected by intraperitoneal injection of about 5 x 10(5) cells. Thirteen injected fish were moribund or died within 4 days with septicaemic lesions. Two survivors were sampled on Day 28 after infection. Yersinia ruckeri was reisolated from the internal organs of all experimental fish. By histopathological examination moribund fish had generalised bacteriaemia with inflammation, degeneration and necrotic foci in kidney, liver and spleen, corresponding to findings described previously in ERM of rainbow trout. Survivors of challenge on Day 28 had a chronic disease characterised by prominent peritonitis and enteritis, exhaustion of the erythroid, granuloid and lymphoid components in haematopoietic kidney tissue as well as focal degeneration and necrosis in organs. These data indicate a high sensitivity of carp to intraperitoneal infection with a relatively low dose of Y. ruckeri.

Animals↗
PubMed1985

Non-Hodgkin's lymphomas of Waldeyer's ring.

The following data have been achieved in investigation of 40 patients with non-Hodgkin's lymphoma involvement of the Waldeyer's ring (WR): a) Palatinal tonsils and nasopharynx are the parts of WR most frequently involved. b) Involvement of WR warrants a careful X-ray, and/or endoscopic examination of the gastrointestinal tract. c) WR is most frequently involved in patients with diffuse histiocytic and lymphohistiocytic NHL. d) The basic treatment in patients of clinical Stage I and II is local-regional radiotherapy, in those of Stage III and IV, it is combined chemotherapy. Of key importance in the choice of the strategy and tactics of treatment is the determination of histological subgroup. e) Prognosis in patients with NHL probably does not depend on the origin of the disease but rather on basic stratification criteria (clinical stage, histological subgroup, occurrence or absence of systemic symptoms, presence or absence of bulky tumors). The results are significantly influenced by the degree of radicality of treatment. Methods of a "local-regional" treatment appear to be inadequate in the majority of patients with NHL of high and intermediate grade malignancy. Consequently, the involvement of WR does not seem to represent a distinct clinical-pathological entity.

Cell Differentiation↗
PubMed1982

Non-invasive methods for detection of cardiomyopathy in the course of antineoplastic treatment.

In our group of patients suffering from cancer and treated by polychemotherapy and monitored in a complex non-invasive way we obtained data as follows: A normal physical, sensoric and biochemical cardiac finding prior to treatment has been observed in 47 patients out of 68 (69.1%). A pathological cardiac finding prior to treatment has been found in 18 patients out of 68 (26.5%). Pathological changes evidencing for cardiomyopathy (CMP) induced by chemotherapy have been detected in 19 patients out of 68 (27.6%). Out of 18 patients with a pathological report prior to treatment in 8 of them the polycardiographic, echocardiographic, and biochemical alterations deteriorated during the chemotherapy. CMP induced by polychemotherapy has been found altogether in 27 patients out of 68 (39.6%). In this group of patients with clinically and biochemically detected CMP due to antineoplastic treatment, we found a significant decrease of ejection fraction (EF) (values less than 50%) and a marked increase Weisler's index (WI) (values greater than 0.520) approximately in 30% of patients. Our preliminary results show that polycardiographic examinations of systolic time interval (STI), the EF detected echocardiographically, as well as determination of myocardial isoenzyme creatine phosphokinase (CK-MB) activity represent useful non-invasive methods of early detection of any myocardial damage in the course of actinochemotherapy. A systematic monitoring of patients reduces the uncertainty with antitumor chemotherapy so that it enables--in a majority of cases--to bring the planned antineoplastic treatment to a successful end.

Antineoplastic Agents↗