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Biomedical subjects

A Bienek

Publications and source records attributed to A Bienek.

4 recordsLinked to original sources

[Sonographic control of wound healing after caesarean section in cattle].

In this study, the use of transcutaneous ultrasonography for the post-operative control of wound healing after caesarean section in cattle was investigated. In 20 cows, admitted to our clinic because of dystocia, a caesarean section was performed via the left flank. Post-operatively, the laparotomy wound was checked with a 5.0 and a 7.5 MHz linear probe every three days until the animal was discharged. In 10 animals, accumulation of fluid was detected ultrasonographically before fluctuation could be palpated in the peri-incision area. The fluid was aspirated after punctuation of the skin and was submitted for microbiological analysis. In 5 cases, sterile haemato-seromas were diagnosed and resorption of the process was followed by repeated ultrasonographic examination. In 5 animals, bacteria could be cultured and in 4 of these cases, development of an abscess was detected with repeated ultrasonography. In one cow, an infected heamatoma was found which remained unchanged, both ultrasonographically and clinically until the animal was discharged. In conclusion, ultrasonography does add important findings to the clinical evaluation of the laparotomy wound after caesarean section and can be used for early diagnosis of a disturbed healing process.

Abscess↗

[Sex-specific changes in body composition during weight reduction].

The aim of this study was to ascertain whether overweight men and women of comparable age, body surface area and weight would display any differences in weight loss or changes in body composition while receiving identical calorie intakes. 15 men and 15 women of mean age 46.7 (26-57) years with obesity (body mass index 29-36 kg/m2) were given a reducing diet containing 700 kcal/d for 4 weeks. The two groups were identical in age, body weight and height. The change in body composition was studied by means of ultrasound and nitrogen balance. The women had thicker layers of subcutaneous fat (241 +/- 8 mm) over all parts of the body than the men (137 +/- 10 mm) (summated measurements at 14 points). Among the men body weight fell from 93.1 +/- 2.1 to 83.8 +/- 2.0 kg, but the women declined only from 92.9 +/- 2.0 to 84.7 +/- 1.9 kg. Although men achieved a greater weight loss (13%; P less than 0.001) the decrease in their subcutaneous fat layer was 33% less (to 117 +/- 9 mm) than in the women (211 +/- 8 mm). Cumulative nitrogen balance among the men was 4.7 times more negative than in women (-104 +/- 14 g versus -22 +/- 11 g); this points to accentuated muscle breakdown. More fat was mobilized in the women than in the men, chiefly from the regions of the limbs and hips.

Adipose Tissue↗

Loss and restoration of preoptic thermoreactiveness after lesions of the rostral raphe nuclei.

Preoptic neurons, extracellularly recorded in the rat's brain, were tested for their responses to thermal stimulation of the scrotal and abdominal skin before and after electrolytic lesions of about 1 mm3 in the area of the rostral raphe nuclei, nucleus raphe dorsalis and centralis (NRD/NRC). All analyzed neurons were of the switching type, i.e. they changed their firing rates to a higher or lower level when a threshold of the peripheral stimulation temperature was exceeded. When major parts of NRD or NRC were destroyed, the preoptic neurons no longer changed their firing rates after thermal stimulation, whereas transmission of noxious information in most cases was not impeded. Smaller lesions in NRD or NRC did not abolish the responses, but brought about essentially modified responses compared to those before the lesions. Lesions lateral to NRD or NRC had no effect. If the lesions were effective and the neurons could be observed for a longer period after the lesions, the response was restored in many cases. As the noxious response had often not been abolished and the lateral lesions were without any effect, it might be that the lesion effects and the restoration of responses involve short-term plasticity. However, temporary block of input to the neurons by unspecific effects cannot be excluded.

Action Potentials↗

The significance of nucleus raphe dorsalis and centralis for thermoafferent signal transmission to the preoptic area of the rat.

Neuronal spike-trains were recorded extracellularly within the preoptic area, which is generally considered the center of information processing for thermoregulation. The neuronal responses were tested by thermal stimulation of the scrotal and abdominal skin of the rat. After a neuron had been identified as warm responsive, electrical stimulation and/or lesion were applied in medial midbrain to test the influence on the preoptic neurons. Post-stimulus-histograms were determined by using 600 single shocks. Furthermore a small area of the medial midbrain was electrolytically lesioned and the thermal response of the neuron was tested again. All lesion, stimulation and recording sites were examined histologically. Electrical stimulation of either nucleus raphe dorsalis or centralis influenced the responsiveness of the recorded preoptic neurons. The post-stimulus-histograms predominantly reveal polysynaptic ascending pathways. Lesion of either nucleus raphe dorsalis or centralis abolished the responsiveness of the recorded preoptic neurons. Lesions lateral to these nuclei were ineffective. By this it is concluded that both nucleus raphe dorsalis and centralis are essentially involved in the thermal signal transmission to the preoptic area.

Afferent Pathways↗