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Biomedical subjects

A Boari

Publications and source records attributed to A Boari.

At least 19 recordsLinked to original sources
PubMed2006

Echocardiographic and Doppler echocardiographic findings in 11 wolves (Canis lupus).

Two-dimensional real-time, M-mode and Doppler echocardiographic measurements were made in 11 adult wolves (Canis lupus) anaesthetised with an intramuscular combination of medetomidine, ketamine, butorphanol and acepromazine followed by isoflurane in oxygen. M-mode measurements of the left ventricle, B-mode measurements of the left atrium and aorta, systolic indices, and Doppler measurements of aortic and pulmonary blood outflow, and of mitral and tricuspid blood inflow, were recorded. The values obtained were compared with those reported for dogs of similar bodyweight and body type. The diastolic measurements of the cardiac chambers and walls were similar to those reported for healthy, conscious dogs, but the use of anaesthesia probably resulted in the markedly different systolic cardiac measurements, systolic indices and Doppler blood flow velocities observed in the wolves. Mild mitral regurgitation, probably due to mitral endocardiosis, was observed in one wolf, and trivial functional mitral insufficiency was observed in five others.

Animals↗
PubMed2000

[Clinical observations on the nature of early caries. A study with scanning electron microscopy].

BACKGROUND: Early caries lesions present a greater porosity in the deep layers than in the superficial layer of the enamel. The "white spot" lesion is a dynamic lesion which is subjected to the changes occurring in the oral cavity; it can regress, stabilize or progress. The aim of this study is to show if there are considerable morphological differences in the superficial ultrastructural aspect of early caries lesions, active or stabilized, in persons of different age. METHODS: To carry out this SEM observation, 15 patients aged from 12 to 50 presenting early caries lesions have been selected. Some of them had been recently subjected to orthodontic treatment, others several years ago. Five of these patients have been called back to examine again the demineralized area by microscope in the very same place of the first observation. The replica technique was used utilizing a silicon-based impression material, an epoxy resin for replicas casting, little steel nets to fix the silicon material. RESULTS: At the level of white spot lesions it is evident a tissural structure scattered with small overlappings and micropits with a diameter of 0.5-2 mu. The patients who removed orthodontic bands more than 10 years before show a shining white aspect, corresponding to the nearly complete loss of enamel perikymata with well defined abrasions, but without any micropits at SEM examination. CONCLUSIONS: The replica technique has shown to be useful to record the defects of active and stabilized caries lesions. However this technique can't be utilized as a diagnostic instrument because it would be too expensive, but it is certainly a valid support for in vivo research.

Adolescent↗
PubMed1997

[Saliva: clinical and diagnostic usefulness. Review of the literature].

Modern clinical pathology is mainly based on the analysis of blood samples, carried out through more and more sophisticated techniques. Being the blood test an invasive technique, it certainly reduces the possibility of carrying out blood analysis, especially in patients such as children, old people, handicapped people and every person who needs continuous biochemical or pharmacological monitoring. In these cases it is very important to find other body fluids that can be easily obtained and that can offer the same reliable data as blood. Due to some of its characteristics, sometime saliva may be a good alternative to venous blood, as it presents the same features of plasma or urine. The chemical analysis of saliva provides some information that are useful for metabolic, inflammatory, neoplastic and genetic diseases; furthermore radioimmunological dosages allow to assess small concentrations of active substances. On the basis of an accurate review of literature, the aim of this study is to analyse the importance of saliva in diagnosing and monitoring certain systemic diseases, the utilization of saliva in hormonal and pharmacological dosages and the use of saliva in some epidemiological studies. Finally, it is emphasized that saliva in monitoring may help to manage several clinical conditions.

Diagnostic Techniques and Procedures↗
PubMed1996

Role of dental plaque in the transmission of Helicobacter pylori infection.

With regard to the role of dental plaque in the transmission of Helicobacter pylori infection, data from the literature vary greatly, owing to differences in sample collection and H. pylori-detecting techniques. Using the polymerase chain reaction (PCR), we have determined the incidence of H. pylori colonization in the dental plaque of 31 consecutive patients who underwent gastroscopy. The patients were divided into two groups on the basis of H. pylori infection, determined by Giemsa stain and the rapid urease test: group A made up of 21 H. pylori-positive patients and group B with 10 H. pylori-negative patients. Our PCR assay of dental plaque samples proved negative in all group A subjects but was positive in only one patient in group B. In our study, we found that H. pylori had a low prevalence (3.2%) in the oral cavity, with no significant relationship between gastric mucosa and dental plaque colonization. More comprehensive studies are needed to determine whether dental plaque is an important reservoir in the epidemiology of H. pylori-induced gastric disease.

Adult↗
PubMed1995

Hypoglycemia in a dog with a leiomyoma of the gastric wall producing an insulin-like growth factor II-like peptide.

A 12-year-old mixed-breed male dog was referred to the Clinica Medica Veterinaria of Bologna University for recurrent episodes of seizures due to hypoglycemia with abnormally low plasma insulin levels (18 pmol/l). Resection of a large leiomyoma (780 g) of the gastric wall resulted in a permanent resolution of the hypoglycemic episodes. Insulin-like growth factors I and II (IGF-I and -II) were measured by RIA in serum before and after surgery and in tumor tissue. Results were compared to the serum concentration of 54 normal and to the tissue concentration observed in eight non-hypoglycemic dog gastric wall extracts. Before surgery, circulating immunoreactive IGF-I was 0.92 nmol/l, which is significantly lower than the control values (16.92 +/- 8.44 nmol/l, range 3.53-35.03), while IGF-II was 152 nmol/l, which is significantly higher than the control values (42.21 +/- 3.75, range 31.99-50.74). After surgery, IGF-I increased to 6.80 nmol/l while IGF-II decreased to 45.52 nmol/l. Tumor tissue IGF-II concentration was higher than normal (5.66 nmol/kg tissue as compared to a range in normal gastric wall tissue of 1.14-3.72 nmol/kg), while IGF-I was 0.08 nmol/kg tissue, which is close to the lowest normal value (range in controls, 0.08-1.18 nmol/kg). Partial characterization of IGF-II immunoreactivity extracted from tissue evidenced a molecular weight similar to that of mature IGF-II, thus excluding that peptide released by the tumor is a precursor molecule.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

[Amalgam fillings and T-lymphocyte changes].

Dental amalgam and nickel alloys have been considered quite safe. Previous authors reported the effect of dental amalgam and nickel alloys on human T-lymphocytes modifications after amalgam dental fillings, into dose-dependence of any modifications and into possible temporary. Eight patients were subjected to dental care with amalgam dental fillings. Drawings of blood were executed at start, fifteen days after late fillings and two months later. The results about modifications of T-lymphocytes were not univocal. We believe, at now, that temporary modifications of the immunity seem to be related to a cytotoxic mechanism.

Adolescent↗
PubMedDate not supplied

Pulsed wave-Doppler ultrasonographic evaluation of the common carotid artery in the resting horse: physiologic data.

A pulsed wave-Doppler ultrasonographic evaluation of common carotid arterial blood flow was carried out on 63 healthy Italian Saddlebred horses. Vessel diameter and tracing morphology were evaluated and blood flow parameters (systolic, diastolic and mean velocity, acceleration and deceleration of the systolic wave, carotid pulse volume) were calculated and correlated with class variables (sex, age and body weight). On the basis of the presence of an incisure in the ascending branch of the systolic curve, subjects were divided in two groups: one with a bifid systolic curve and the other with a monophasic aspect. Correlations between: 1) diameter of the vessel and body weight and 2) carotid pulse volume and flow velocity (systolic, diastolic and mean) were found. A greater systolic pulse volume was found in male subjects, in subjects with greater body weight and in those which had a monophasic systolic wave.

Acceleration↗
PubMedDate not supplied

Bonding of composite resins to dentin: SEM observation of one-component enamel-dentin bonding systems.

AIM: To evaluate in vitro the qualitative characteristics of the surfaces of some enamel-dentin bonding systems. METHODS: Seven different bonding systems were observed first by stereomicroscopy then by scanning electron microscopy, as follows: 49 extracted teeth were abraded to expose a uniform dentin surface. After application of the bonding system, composite resin was placed on the surface and shaped as an inverted truncated cone. The tensile bond test was performed and the specimens observed under SEM; the fracture location was determined and the formation of the hybrid layer evaluated. RESULTS: SEM observation indicated that adhesive, cohesive and mixed fractures had occurred. The abraded dentin surfaces were also observed under SEM before and after etching. The most recent enamel-dentin bonding systems use a conditioning technique that demineralizes the upper dentin surface at and around the tubule entrance. This is known as the total etching technique. A layer is formed in which the demineralized dentin is impregnated with fluid resin: the so-called hybrid layer. CONCLUSIONS: The length of the plugs of adhesive was not found to be determinant. The formation of the hybrid layer appeared to be more important, as it indicated a three-dimensional interaction between the conditioned collagen fibres and the primer that diffuses the adhesive.

Acid Etching, Dental↗
PubMedDate not supplied

[Efficacy of 4 adhesive systems for amalgam. In vitro study].

BACKGROUND: The aim of this research is to study the shear bond strength of 4 adhesive systems of the last generation: All-Bond 2 (AB2), Clearfil Liner Bond 2 (CL), Scotchbond MP plus (SBMP) and High Q Bond plus (HQB). According to the manufacturing company these products are suitable for amalgam adhesion to dentin. METHODS: Forty extracted human third molars have been ground to expose a flat dentin surface. The teeth were randomly assigned to 4 groups, and every group was treated with one of the adhesive system according to the manufacturer's instructions. Samples were then secured in a split Teflon mould, having a 6 mm diameter opening and a spherical amalgam was triturated and condensed onto the treated dentin surfaces. The amalgam cylinders have been exposed to a tensile bond test using a Universal Testing Machine (Instron 4500). ANOVA test has been used to the statistic study among groups. RESULTS: The study of the tensile mean range, showed that dentin shear bond strength values (MPa) for the adhesive systems tested were: 8.13 +/- 1.48 for AB2, 7.06 +/- 1.41 for SBMP, 4.2 +/- 1.06 for HQB, 3.04 +/- 0.68 for CL. The statistic test revealed significant differences among the 4 groups (p < 0.05). CONCLUSIONS: The bond strength mean values of amalgam adhesives are lower than the ones for composite resins, nevertheless dentin-enamel adhesive systems specific for amalgam have to be taken into consideration particularly in case of conservative restorations that need chemical retention as a support, when the mechanical one is not enough.

Dental Amalgam↗
PubMedDate not supplied

Granular cell tumor of the tongue (Abrikossoff's tumor). A case report and review of the literature.

Granular cell tumor (GCT), also known as Abrikossoff's tumor, is a relatively uncommon neoplasm presenting as a benign, single, well-circumscribed nodule, usually arising on the tongue. Histologically, GCT shows numerous strands of large polyhedral granular cells, separated by collagen bundles, with no evidence of encapsulation. Recurrences may occur following inadequate excision. A 32-year-old woman, presenting a non-painful nodule embedded in the chorion of her tongue, underwent a first excisional biopsy which revealed a GCT and the margins of the bioptic sample appeared free. Immunohistochemistry for protein S-100 revealed the tumor cells invading the margins of the sample. A second intervention was made to excise the possible remaining neoplastic tissue. This case, owing to its uncommon clinical appearance, seemed particularly interesting. Immunohistochemical assay for S-100, whenever a CGR is suspected, is always mandatory to discover tumor cells infiltrating the margins of the sample. Furthermore, immunohistochemical assay can make the diagnosis more precise excluding other more serious tumors which could arise in the oral cavity. In any event, the patient should be reassured of the benign nature of the tumor and a careful follow-up is necessary in order to diagnose relapses.

Adult↗