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Biomedical subjects

A Borrè

Publications and source records attributed to A Borrè.

18 recordsLinked to original sources
PubMed1996

c-Myc inactivation by mutant Max alters growth and morphology of NCI-H-630 colon cancer cells.

The myc gene family has been implicated in multiple cell processes including proliferation, differentiation, tumorigenesis, and apoptosis. For its cellular growth promoting function, Myc must heterodimerize with Max. To study the effect of Myc inactivation on the growth and differentiation properties of epithelial tumor cells, we transfected the H-630 human colon cancer cell line with bm-max, a mutant Max protein in which DNA-binding activity has been abolished. Cells expressing high levels of bm-Max grow poorly, and the morphology of both colonies and single cells is altered. Moreover, increased bm-Max expression results in a prolonged G alpha/G1 phase accompanied by induced expression of p21 (WAF1/CIP1), elevated levels of alkaline phosphatase (ALP) activity, and accumulation of large fat granuli within the cells. These distinctive cell characteristics are associated with differentiation processes in numerous malignant cell lines. The results of this study support a model in which sequestering of endogenous Myc and Max proteins into "basic mutant" dimers lacking DNA-binding activity is sufficient both to inhibit proliferation and to induce changes in cell behavior consistent with differentiation.

Alkaline Phosphatase↗
PubMed1995

[Ultrasonographic study of the patellar tendon following bone-tendon-bone anterior cruciate ligament reconstruction arthroscopy].

The arthroscopic reconstruction of the anterior cruciate ligament requires the partial removal of the patellar tendon. US potentials in the assessment of residual patellar tendon after bone-tendon-bone removal were compared with those of CT and clinics. Mechanical resistance reduction of the residual tendon may be caused by removal modalities and size. This work was aimed at investigating the morphologic US patterns indicating "stability" of the operated tendon, which is mandatory for a risk-free functional recovery. Twenty patients were submitted to US of the operated tendon with contralateral comparison. In each patient tendon thickness and structure in the site of removal were studied, especially aiming at demonstrating diffuse or focal alterations of echogenicity. Middle third thickness and new tissue echostructure are fundamental US variables to define residual tendon "stability". The authors describe some US patterns of the surgical tendon to be considered as normal and not abnormal findings, provided that US be performed at least two months after surgery.

Adolescent↗
PubMed1993

Type II interleukin-1 receptor is not expressed in cultured endothelial cells and is not involved in endothelial cell activation.

Interleukin-1 (IL-1) profoundly affects a number of functions of endothelial cells (EC). It was previously shown that EC express the type I 80-Kd IL-1 receptor (IL-1 RI). In this study we define the expression and functional significance of the type II IL-1R (IL-1 RII) in EC. Human umbilical vein EC did not express appreciable levels of IL-1 RII mRNA as assessed by Northern analysis or reverse transcription and polymerase chain reaction. Exposure to various cytokines (including IL-4, which augments IL-1 RII in neutrophils) failed to induce IL-1 RII mRNA. The binding of radiolabeled IL-1 beta to EC was blocked by antitype I (M4) but not by antitype II (M22) monoclonal antibodies (MoAbs). MoAbs directed against the IL-1 RI (M1 and M4) inhibited the induction of IL-6 and adhesion molecules in EC by IL-1, whereas an anti-IL-1 RII (M22) was inactive. The human IL-1 receptor antagonist (IL-1ra) preferentially interacts with IL-1 RI versus IL-1 RII in the mouse. IL-1 ra inhibited the response of mouse endothelial cells to IL-1. We conclude that EC selectively express the IL-1 RI and that this is involved in the response of this cell type to IL-1.

Animals↗
PubMed1992

Interleukin 4 amplifies monocyte chemotactic protein and interleukin 6 production by endothelial cells.

Upon exposure to interleukin 4 (IL-4), human umbilical vein endothelial cells (EC) produced low amounts of IL-6 and expressed low levels of IL-6 transcripts. While being a weak stimulus for IL-6 production, IL-4 amplified the release and mRNA expression of this cytokine in response to optimal concentrations of IL-1. IL-4 also induced low levels of monocyte chemotactic protein but amplified the expression of this chemoattractant in response to inflammatory signals. Thus IL-4, by amplifying at the level of the vascular endothelium the expression of vascular cell adhesion molecule 1 selectively, as well as that of chemoattractants and IL-6, may favour the selective recruitment and activation of lymphoid and monocytic elements crucial for subacute/chronic inflammation and immune reactions.

Cell Adhesion Molecules↗
PubMed1992

[Pneumothorax in multiple trauma. Radiologic and CT study].

This study was aimed at evaluating the necessity to perform chest Computerized Tomography (CT) in multiple traumatized patients to diagnose pleuropulmonary lesions and, particularly, pneumothorax: the correct identification of this condition, although minimal, is important especially in prevision of long anesthesias and/or positive end-expiratory pressure (PEEP) therapy. This assisted respiratory technique improves arterial oxygenation but causes a barotrauma which may cause some complications; particularly, a small undetected pneumothorax can suddenly increase so as to cause pulmonary collapse with sometimes dramatic symptoms. Chest X-ray films and CT scans, performed in rapid succession on patient's admission in Emergency Ward, were compared in 21 subjects. CT is indispensable in case of severe chest parietal lesions which can mask the radiological evidence of pulmonary or pleural conditions, as it occurred in 3 of our cases. Moreover, CT resulted more reliable than chest X-rays (18 versus 10 correct diagnoses) especially in the detection of small antero-inferior pneumothorax flaps, in which direct radiological signs cannot be identified, in default of radio-geometrical assumptions. Indirect radiological signs of pneumothorax must be recognized but critically considered in order to avoid over-staging.

Adolescent↗
PubMed1991

[Ultrasonography in diseases of lymph nodes and soft tissues of the neck].

A study of 55 patients affected by neoplasia of the head and neck or clinically evident laterocervical swellings was carried out in order to evaluate the diagnostic value of ultrasonography in the study of cervical lymph nodes and soft tissues, excluding thyroid or salivary pathologies. The analysis of results confirmed that, in line with previously published findings, ultrasonography is a reliable method of diagnosing lesions and correctly identifying the affected organ, but then it is considerably less reliable in defining the type of lesion. In view of this, it is reasonable to propose a more widespread use of ultrasonographically guided needle biopsy which, in addition to being a rapid and non-traumatic method, would allow precisely target samples to be taken from those lesions with a doubtful or unusual clinical or ultrasonic appearance.

Adolescent↗
PubMed1989

[The clinical prognostic importance of routine study of the aorta during abdominal echotomography in the elderly patient].

Aneurysm of the abdominal aorta (AAA) is encountered with increasing frequency and is often observed at fixation or rupture, events which can be treated surgically but suffer from high operative mortality. Given the current tendency to extend the surgical indication to increasing sectors of patients, the clinico-prognostic significance of routine study of the abdominal aorta with echotomography has been investigated. This examination is reliable in all elderly patients who undergo ultrasound examination for different clinical indications. In the 147 patients considered, aged more than 60 and of both sexes, 12 AAA not suspected clinically were observed. For this the surgical indication or echotomographic monitoring was discussed in relation to age and clinical condition and with due consideration for the morphologic volumetry of the lesion. The finding of the 12 cases of AAA is an indicator of the usefulness of a routine study of the aorta in elderly patients, for awareness of the lesion means that the best therapeutic decisions can be taken and makes it possible to act effectively in emergency situations such as fixation, rupture and distal embolism.

Abdomen↗
PubMed1988

[Ultrasonic evaluation of primary hyperparathyroidism].

The authors evaluated the diagnostic reliability of echotomography in detecting parathyroid masses: 37 patients were studied with primary hyperparathyroidism, in four cases recurrent. They all underwent surgical ablation of the mass, identification of all glands, and biopsy of an apparently normal parathyroid. Echotomography was accurate in 91.4% of cases: it identified 27 out of 39 pathological glands (sensibility 69.2%) and gave no false-positive results (specificity 100%). Since parathyroid is often ectopic, oblique scans are suggested, i.e. of jugulum while the patient is swallowing. Such scans were always used in our study, in order to identify lesions even in case of "difficult" locations, such as the superior mediastinum.

Adenoma↗
PubMed1988

[A possible role of magnetic resonance in the follow-up of renal transplants].

The authors discuss the possible role of Magnetic Resonance Imaging (MRI) in the follow-up of renal transplants. Its diagnostic accuracy is compared with Ultrasounds (US), which is considered the method of choice in postoperative monitoring of renal transplants. Thirty-two patients (transplant life ranging from 5 days to 37 months) were examined in the same day with both MRI and US. Sonographic evaluation is based on a group of signs related to renal alterations and unanimously ascertained; the identification of MR patterns of normally functioning kidney or renal rejection might give way to more extensive clinical applications of the method, with the advantages of multiplanarity and no X-ray use. MR diagnostic accuracy in the diagnosis of normally functioning transplant or renal rejection was 82.7% (vs. US: 68.9%); even though specificity was the same (84.6%). MRI had higher sensibility than US (82.25% vs. 656.25%). MRI is more sensitive in demonstrating renal pathology in transplanted kidneys, and its diagnostic accuracy is superior. Still, US must be considered the methodology of choice in instrumental research when there is a clinical suspicion of renal rejection, due to its sufficient diagnostic accuracy, to its being easy to perform in continuous follow-up, to its low cost and to the short execution time. MRI is nevertheless suggested in the follow-up of renal transplants both in questionable cases and when biopsy cannot be performed.

Adolescent↗
PubMed1988

[Disorders of heart rhythm during urography studied with Holter monitoring. Comparison of ionic and non-ionic contrast media].

Given the disagreements in the literature over the extent of cardiac complications during urography it was decided to assess any arrhythmias following the injection of ionic and non-ionic contrast media and a placebo by means of 24 hours dynamic Holter monitoring. The results were subjected to meticulous statistical analysis and showed no connection between electrocardiographic alterations and the substance injected. The absence of statistical significance and the variability of the results suggest that alterations in the physiological parameters and/or arrhythmias arising during the administration of contrast media should be assessed with extreme caution.

Adolescent↗
PubMed1987

[Diagnostic accuracy of sequential angio-urography with the spot-camera in clinical practice].

In this perspective study the authors examined 57 patients to evaluate diagnostic accuracy of sequential angiourography (AUS) using spot-camera and image subtraction for the visualization of the vascular phase of the renal arteries. All the subjects underwent surgery or follow-up with other clinical and radiological methods. The results obtained with this technique, of low cost and simple execution, were satisfactory: accuracy 98%, sensitivity 100%, specificity 98%.

Adolescent↗
PubMed1985

[Experimental dosimetric comparison between single tomography and multiple simultaneous tomography with Synchroplan in clinical practice].

Reference is made to a dosimetric comparison between single tomography and simultaneous multiple tomography with Synchroplan in 10 patients subjected to nefroangiotomography. Data from several thermoluminescence dosimeters placed on different parts of the body were used in a careful statistical analysis that showed the absence of significant differences in absorbed dose in function of constitutional habitus. It was also found that the dose absorbed for 6 tomograms of the renal cavities in single tomography was about 4 times higher than with the Synchroplan (using a previously experimented set of high-sensitivity intensifying screens), which provides 6 tomograms in the same dynamic phase at different levels for the same exposure.

Body Constitution↗
PubMed1983

[Proposal of an echotomographic method of guided portal catheterization].

To identify the frontal plane on which to find the right branch of the portal vein in 33 patients who underwent transhepatic percutaneous portography, we used a real-time ultrasonic scanner improved with an original device conceived for this specific need. The suggested method reduced the number of the ineffectual punctures of the liver, allowing in nearly all cases the cannulation of the right branch on the first attempts.

Adult↗
PubMedDate not supplied

[Possibility of using the spot camera in the documentation of the arterial phase in urography].

32 patients have been examined with sequential angiourography (AUS) and image subtraction, utilizing a spot-camera instead of a rapid seriograph, for the visualization of the vascular phase of the renal artery. Satisfactory results have been obtained since in 96.9% of the cases (31/32) it has been possible to obtain representation of those structures sufficient for a clinical evaluation. This technique for its low cost and simplicity of execution can therefore be proposed as a routine examination in the suspect of vascular renal pathology. This method and the more diffuse AUS with rapid seriograph offer, with respect to digital subtraction angiography (DSA), the advantage of a better nephrographic and urographic phase due to the higher quantity of contrast medium injected.

Adolescent↗