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Biomedical subjects

A Brüngger

Publications and source records attributed to A Brüngger.

13 recordsLinked to original sources
PubMed2000

Comparative microarray analysis of gene expression during apoptosis-induction by growth factor deprivation or protein kinase C inhibition.

The transcriptional response of mouse pro-B cells to two different apoptotic stimuli was investigated. First, interleukin-3 (IL-3) deprivation was used to trigger programmed cell death in IL-3 dependent FL5.12 cells. Alternatively, cells were treated with the protein kinase C (PKC) inhibitor staurosporine. The temporal pattern of gene expression was followed with cDNA microarrays, covering over 8700 different mouse cDNA sequences corresponding to approximately 7900 unique genes. Messenger RNA levels of 315 genes were found to be regulated by more than twofold upon IL-3 removal, while 125 genes reacted to staurosporine treatment. Cross-comparison revealed an intersection of 34 genes similarly regulated in both pathways and thus representing candidates for common apoptosis regulators. For many expressed sequence tags (ESTs) our data suggest for the first time functions in the control of apoptosis, stress response or the cell cycle. IL-3 removal led to the repression of genes required for proliferation and to the induction of genes, linked to apoptotic and signaling pathways. Staurosporine caused predominantly activation of genes, some of which had previously been described to be involved in inflammation. Our findings indicate that cellular responses to both apoptotic stimuli influence various physiological pathways which had not previously been known to be linked.

Animals↗
PubMed1994

[Transferrin concentration in seminal plasma in testicular varicocele with various sperm concentrations and various forms of azoospermia].

Seminal plasma transferrin concentrations were measured in semen samples from 424 men attending a fertility unit. There was a clear positive correlation between seminal plasma transferrin concentration and sperm density: transferrin concentrations decrease with decreasing sperm density and are lowest in patients with azoospermia and those who have undergone vasectomy. The differences between the various groups in sperm density are highly significant, but individual data counts also vary widely. Furthermore, it was demonstrated that in polyzoospermia the seminal plasma transferrin concentration is increased, suggesting primary tubular hyperactivity. No correlations with other seminal parameters or hormonal values were found. Seminal plasma transferrin concentrations in normozoospermia and in varicocele testis or in cases with increased numbers of immature germ cells in the ejaculate were not significantly different. This may be interpreted as indicative of intact secretory activity of Sertoli cells in both varicocele and increased desquamation of immature germ cells. The absence of correlation with any of several important spermatological parameters and our inability to differentiate between azoospermia caused by obstruction and by tubular impairment indicate that seminal plasma transferrin is not a useful marker for Sertoli cell function or for seminiferous tubular dysfunction.

Adolescent↗
PubMed1990

Kaposiform acro-angiodermatitis with arteriovenous malformation (Stewart-Bluefarb syndrome).

A case of acro-angiodermatitis with congenital arteriovenous fistulae (Stewart-Bluefarb syndrome) at the distal right foot in a young man is reported. The lesion evolved slowly from a small dark-red spot existing from birth. Besides mechanical disturbances, the patient's only symptoms were a warmer and somewhat bigger right foot compared to the left nonaffected side. He presented a circumscribed spongy, livid-red coloured angiomatous lesion at the upper part of the distal right foot, also involving the proximal parts of the three middle toes. The diagnosis was based on the clinical aspect and the findings by Doppler ultrasonographic and oscillographic examination. The transcutaneous oxygen pressure showed a marked hypoxia at the edge of the acro-angiodermatitis. Finally, arteriovenous fistulae were demonstrated by arteriography.

Acrodermatitis↗
PubMed1989

[Benign prostatic hyperplasia: a stromal disease].

Morphometric studies show that benign human prostatic hyperplasia is a stromal disease caused by the activation of smooth muscle cells. This activation manifests itself in increased amounts of cytoplasmic organelles, which are preferably localized in the perinuclear region. Moreover, marked vesicular activity is present. Besides considerable overproduction of type I and III collagens, the architecture of the extracellular matrix is altered distinctly. In spite of strong evidence that androgens and estrogens regulate the growth of epithelial and stromal cells in the prostate and the induction of fibromuscular overgrowth in various animal models, the exact role of steroids in the pathogenesis of benign prostatic hyperplasia still remains to be elucidated.

Androgens↗
PubMed1989

[Transcutaneous measurement of oxygen and carbon dioxide pressure in necrobiosis lipoidica].

Transcutaneous measurement of oxygen pressure (PcO2) and carbon dioxide pressure (PcCO2) was performed in nine patients with histologically confirmed necrobiosis lipoidica. None of the patients had diabetes mellitus. All measurements were taken at the lower leg. In each case, the atrophic center, the inflamed border, and the surrounding clinically normal skin of necrobiosis lipoidica were examined at 44 degrees C sensor temperature (maximal vasodilatation). Statistically significant hypoxia was found in the area of necrobiosis lipoidica, which was even more pronounced in the inflamed border. Inhalation of 100% oxygen provoked a marked increase in the PcO2 in the lesion, but the values were still significantly lower than in the normal skin. At the edge of the lesions the PcCO2 was significantly elevated. These findings support a vascular origin of necrobiosis lipoidica, involving reduced vascular perfusion combined with diffusion block.

Adult↗
PubMed1989

[Carcinoma spinocellulare and cutaneous T-cell lymphomas].

Two cases of squamous cell carcinoma in patients with cutaneous T cell lymphoma are presented, together with a survey of the rather sparse literature on this subject. The cases presented showed considerable differences in their course. The mutagenic effects of the therapies used against the lymphoma are claimed to be responsible for the development of squamous cell carcinoma in almost all the published reports. However, we think the disturbances of the immune system caused either by the lymphoma itself or by the therapy might be a decisive factor in the development of secondary tumors. This possibility is supported by a comparison of immunosuppressed renal transplant recipients, who frequently develop squamous cell carcinomas of the skin. Frequent clinical follow-up examination of patients with cutaneous T cell lymphomas and early biopsy of suggestive lesions seem mandatory for differentiation between squamous cell carcinomas and tumorous infiltrations of the primary lymphoma. Radical surgical excision, which allows histological examination of the tumor margins, is the therapy of choice for these frequently very aggressive squamous cell carcinomas.

Aged↗
PubMed1987

Cryofibrinogenemic purpura.

A 50-year-old male patient had three episodes of acrally located purpura within 12 years without any other symptoms. The first two episodes which lasted several months coincided with the intake of beta-blockers; the third episode developed after a respiratory infection. A cryofibrinogenemia was found without any evidence of an underlying primary disease, especially not of a neoplasm. The clinical, histological, immunohistological, and electron microscopical findings, as well as the results obtained by intravital fluorescence videomicroscopy are presented.

Cryoglobulinemia↗
PubMed1987

Correlation of biochemical (receptors, endogenous tissue hormones) and quantitative morphologic (stereologic) findings in normal and hyperplastic human prostates.

In previous light and electron-microscopic analyses human benign prostatic hyperplasia was shown to be predominantly a stromal disease; the aim of the present study was to correlate the stereological data with the levels of the endogenous tissue hormones (androgens, estrogens, progesterone) in normal (N) and hyperplastic human prostatic tissues (BPH). BPH tissue specimens were obtained by open prostatectomy (n = 25); normal prostatic tissue was obtained from kidney donors (n = 5). No statistically significant difference was found between normal and hyperplastic tissue. Testosterone BPH 0.69 +/- 0.44, N 0.25 +/- 0.12; 5 alpha-dihydrotestosterone BPH 7.0 +/- 2.9, N 4.2 +/- 0.7; progesterone BPH 0.059 +/- 0.022, N 0.058 +/- 0.005; estrone BPH 0.10 +/- 0.03, N 0.14 +/- 0.03; estradiol BPH 0.07 +/- 0.02, N 0.05 +/- 0.02; estriol BPH 0.02 +/- 0.01, N 0.04 +/- 0.02. Using a Spearman rank correlation coefficient a statistical analysis was performed for age, weight of the prostate, absolute stereological data and the endogenous prostatic hormones. As can be seen from the statistical analysis there is a poor correlation for 5 alpha-dihydrotestosterone and the amount of the glandular epithelium; otherwise no correlation of the endogenous tissue hormones with the stereological data investigated was found. These data show that the stromal overgrowth of benign hyperplasia is not reflected in the tissue hormone levels.

Gonadal Steroid Hormones↗
PubMed1987

[Skin sarcoidosis simulating necrobiosis lipoidica].

A patient who had had pulmonary sarcoidosis for 11 years developed multiple symmetrical lesions with ulcerations on the lower legs. The lesions showed the typical clinical picture of necrobiosis lipoidica and could not be differentiated histologically with certainty. Three years later persistent reddish plaques appeared on both arms, which allowed clinical and histological diagnosis of cutaneous sarcoidosis.

Biopsy↗