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A Brookes

Publications and source records attributed to A Brookes.

21 records · Page 2Linked to original sources
PubMed1984

Electrophysiological evidence for kappa-agonist activity of dynorphin in rat brain.

Dynorphin (1-13) and the mu-agonist, FK 33,824, have been applied microiontophoretically to single neurones in the hippocampus of the rat. Whereas FK 33,824 predominantly caused an increase in neuronal firing rates, dynorphin (1-13) decreased activity. Both effects were blocked by the opiate antagonist naloxone, whereas the effects induced by GABA and glutamate were not. This action of dynorphin (1-13) is comparable to that of other kappa-agonists in this brain region and suggests that dynorphin (1-13) may be a ligand for the kappa opiate receptor.

Animals↗
PubMed1984

A microiontophoretic study of the actions of mu-, delta- and kappa-opiate receptor agonists in the rat brain.

The actions of mu-, delta- and kappa-opiate receptor agonists have been compared on the activity of single neurones in the brain stem, caudate nucleus and hippocampus of the rat, using the technique of microiontophoresis. In the brain stem and caudate nucleus the predominant effect of all the opiate agonists tested was depression of neuronal activity which was antagonized by naloxone. The selectivity of naloxone as an opiate receptor antagonist was indicated by its lack of effect on gamma-aminobutyric acid (GABA)-induced responses. In the hippocampus both mu- and delta-agonists mainly caused an increase in neuronal firing rates, though some neurones were depressed. In contrast, all the kappa-agonists, including the proposed endogenous ligand for the kappa-receptor, dynorphin, caused depression of neuronal activity. All of these effects were antagonized by naloxone. There was a clear distinction in the areas within the hippocampus in which the mu- and delta-agonists produced different effects. Neurones in the pyramidal cell layer were always excited by these drugs, whereas neurones in the granule cell layer of the dentate gyrus were always depressed by the same drug.

Action Potentials↗