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Biomedical subjects

A Browning

Publications and source records attributed to A Browning.

16 recordsLinked to original sources
PubMed2006

Syphilitic acute posterior placoid chorioretinitis in nonimmuno-compromised patients.

PURPOSE: To describe the clinical and angiographic features of three cases of secondary syphilis in immunocompetent patients, which presented as acute posterior placoid chorioretinitis (APPC) to the ophthalmologist. METHODS: Interventional case series. The aetiology of the APPC was confirmed by serology to be secondary syphilis. Optical coherence tomography, electrophysiology, fundus fluorescein, and indocyanine green (ICG) angiography were performed at presentation and after resolution. Appropriate treatment for secondary syphilis was instituted in each patient. RESULTS: The clinical features, fundus fluorescein and ICG angiography, multifocal electroretinography (mfERG), and optical coherence tomography findings of APPC are described. All three patients had a satisfactory resolution of the APPC with improvement in visual acuity. CONCLUSIONS: APPC in secondary syphilis can occur even in immunocompetent patients. A high index of suspicion is required for early diagnosis of this condition resulting in a good visual outcome with adequate treatment. mfERG and optical coherence tomography are useful in the diagnosis and follow-up of these patients.

Adult↗
PubMed2006

Lack of value of the Martius fibrofatty graft in obstetric fistula repair.

OBJECTIVE: To ascertain if the Martius graft is of benefit to successful surgical outcome in obstetric fistula repair. METHOD: A retrospective analysis of 440 consecutive obstetric fistula repairs performed by the author in the Addis Ababa Fistula Hospital, Ethiopia or the Barhirdar Hamlin Fistula Center, Ethiopia, with or without the Martius fibrofatty graft. These were further divided into 13 subgroups of vesicovaginal fistula and all groups compared against two outcomes; fistula closure and presence of residual urethral incontinence following repair. Results were statistically analyzed with the Fisher's exact or Chi-squared test. RESULTS: There was no statistical difference between groups with regards to breakdown of repair but a higher rate of residual incontinence in 3 subgroups with the Martius graft. CONCLUSION: The Martius graft can be safely omitted from obstetric fistula repair if the surgeon is experienced, and performs the same type of repair as used in this analysis.

Adipose Tissue↗
PubMed2006

A new technique for the surgical management of urinary incontinence after obstetric fistula repair.

Obstetric vesicovaginal fistula is a debilitating injury still occurring widely throughout the developing world. Fortunately, now more and more surgeons are becoming aware of this previously hidden problem, and many thousands of women are receiving treatment each year. What is difficult for the fistula surgeon are those women, who after having their vesicovaginal fistula successfully closed, return to their doctor, still just as incontinent as they were with the fistula still open. This study describes a new, simple and cheap surgical procedure that promises to improve the lot of these unfortunate women.

Adolescent↗
PubMed2006

Risk factors for developing residual urinary incontinence after obstetric fistula repair.

Obstetric vesicovaginal fistula is a devastating cause of urinary incontinence worldwide. More attention is being focused on treating these patients by various philanthropic societies but very little is known about the complications of surgery. One of the most common complications is that the woman is left with residual urinary incontinence despite the defect being successfully closed. If risk factors for this could be identified, then perhaps more appropriate surgical techniques could be developed with the aim of remedying the incontinence.

Adolescent↗
PubMed2006

Effect of age related macular degeneration on the Eger macular stressometer photostress recovery time.

AIM: To assess the repeatability of Eger macular stressometer (EMS) measures of photostress recovery and determine their association with other measures of visual function. METHODS: EMS photostress recovery time was measured in 90 patients with bilateral exudative age related macular degeneration (AMD), 19 with bilateral atrophic AMD and 47 with both forms of the condition (mean age 79 (SD 13) years). Measurements were made on two occasions separated by 1 year. Intrasession repeatability was assessed by repeating the measures after a 10 minute recovery period at the first visit. Distance visual acuity was measured with a logMAR chart, near visual acuity with a MNRead chart at 25 cm, contrast sensitivity with a Pelli-Robson chart, and the presence of central visual disturbance assessed with an Amsler grid. A questionnaire was used to assess self reported difficulties with glare recovery. RESULTS: The average EMS recovery time was 11.0 (SD 8.9) seconds, decreasing by 1.6 (5.2) seconds on repeated measurement (p<0.05). EMS photostress recovery was not correlated with visual function measures or subjective difficulties with lights (p>0.05). EMS photostress recovery time did not predict those whose vision decreased over the following year compared with those among whom it remained stable. CONCLUSIONS: The EMS test is not a useful tool in determining the severity or progression of AMD.

Adaptation, Ocular↗
PubMed2003

Proposal for a tiered approach to developmental toxicity testing for veterinary pharmaceutical products for food-producing animals.

In order to establish the safety of veterinary drug residues in human food, a number of toxicological evaluations are required, including the assessment of potential risks to development. In the light of the use of developmental toxicity testing for risk characterization, we evaluated whether conducting these tests in more than one species was redundant. Review of the published Summary Reports of recommendations of the EU Committee for Veterinary Medicinal Products supplemented with data from the Joint FAO/WHO Expert Committee on Food Additives (JECFA) reports on veterinary drug residues in food identified 120 compounds, of which 105 had teratogenicity information in more than one species. The analysis of these compounds, representing a variety of chemical and pharmacological classes, provided justification for consideration of the use of a tiered approach for developmental toxicity evaluation of veterinary drugs for food-producing animals. The tiered approach begins with developmental toxicity testing in a rodent species, preferably the rat. If teratogenicity is observed, no testing in a second species would be required, except under specific circumstances where the ADI is determined based on the NOEL from this study. If a negative or an equivocal result for teratogenicity were observed in the rodent, then a developmental test in a second species, preferably the rabbit, would be conducted. The tiered approach provides thorough hazard identification, based on the use of a second species for compounds negative for teratogenicity in the rodent, and maintains maximum public protection based on the extremely low potential for human exposure to these compounds, while making a genuine attempt to limit unnecessary animal testing.

Abnormalities, Drug-Induced↗
PubMed2000

The effects of hypergravity and substrate vibration on vestibular function in developing chickens.

We used linear vestibular evoked potentials (VsEPs) to characterize peripheral and central vestibular function in birds following embryogenesis at 2G centrifugation or at elevated levels of vibration (+20dB re: background levels). Additionally, we characterized peripheral and central vestibular adaptation to 2G centrifugation in early post-hatch birds. Linear VsEP response peak latencies, amplitudes, thresholds and input/output functions were quantified and compared between experimental and control animals. Birds vibrated throughout embryogenesis and up to one-week post-hatch revealed no changes in linear VsEP response components compared to control siblings. Birds centrifuged at 2G throughout embryogenesis also evidenced no changes in the linear VsEP measured at hatch (P0). Significant changes were seen, however, for linear VsEPs of post-hatch birds placed at 2G for 7 days beginning on post-hatch day 5. Linear VsEPs for these animals displayed significant reductions in response amplitudes associated with peaks P2, N2 and P3, response peaks generated by central neural relays of gravity receptors. The earliest response components, generated by the peripheral vestibular nerve (i.e., P1, N1), were not significantly altered with the 7-day exposure to 2G. Thus, there was no evidence of generalized changes in peripheral gravity receptor excitability or in the rate of maturation in developing animals under increased levels of gravity or vibration. If gravity level plays a critical role in shaping peripheral vestibular ontogeny at magnitudes between 1 and 2G, then it may serve to stabilize function under changing G-fields or it may operate on physiological features that can not be resolved by the VsEP. In contrast, exposure to elevated gravity during post-hatch periods does alter central vestibular function thus providing direct evidence for central vestibular adaptation to the gravitational environment. The fact that central functional change was observed in hatchlings and not embryos, raises the possibility that the first 2-weeks post-hatch may be a critical period of "heightened developmental sensitivity" to hypergravity.

Adaptation, Physiological↗
PubMed1999

The representation of pleasant touch in the brain and its relationship with taste and olfactory areas.

Although there has been much investigation of brain pathways involved in pain, little is known about the brain mechanisms involved in processing somatosensory stimuli which feel pleasant. Employing fMRI it was shown that pleasant touch to the hand with velvet produced stronger activation of the orbitofrontal cortex than affectively neutral touch of the hand with wood. In contrast, the affectively neutral but more intense touch produced more activation of the primary somatosensory cortex than the pleasant stimulus. This indicates that part of the orbitofrontal cortex is concerned with representing the positively affective aspects of somatosensory stimuli, and in further experiments it was shown that this orbitofrontal area is different from that activated by taste and smell. The finding that three different primary or unlearned types of reinforcer (touch, taste, and smell) are represented in the orbitofrontal cortex helps to provide a firm foundation for understanding the neural basis of emotions, which can be understood in terms of states elicited by stimuli which are rewarding or punishing.

Brain↗
PubMed1998

The neurophysiology of taste and olfaction in primates, and umami flavor.

To investigate the neural encoding of glutamate (umami) taste in the primate, recordings were made from taste responsive neurons in the cortical taste areas in macaques. Most of the neurons were in the orbitofrontal cortex (secondary) taste area. First, it was shown that there is a representation of the taste of glutamate which is separate from the representation of the other prototypical tastants sweet (glucose), salt (NaCl), bitter (quinine) and sour (HCl). Second, it was shown that single neurons that had their best responses to sodium glutamate also had good responses to glutamic acid. Third, it was shown that the responses of these neurons to the nucleotide umami tastant inosine 5'-monophosphate were more correlated with their responses to monosodium glutamate than to any prototypical tastant. Fourth, concentration response curves showed that concentrations of monosodium glutamate as low as 0.001 M were just above threshold for some of these neurons. Fifth, some neurons in the orbitofrontal region, which responded to monosodium glutamate and other food tastes, decreased their responses after feeding with monosodium glutamate to behavioral satiety, revealing a mechanism of satiety. In some cases this reduction was sensory-specific. Sixth, it was shown in psychophysical experiments in humans that the flavor of umami is strongest with a combination of corresponding taste and olfactory stimuli (e.g., monosodium glutamate and garlic odor). The hypothesis is proposed that part of the way in which glutamate works as a flavor enhancer is by acting in combination with corresponding food odors. The appropriate associations between the odor and the glutamate taste may be learned at least in part by olfactory to taste association learning in the primate orbitofrontal cortex.

Animals↗
PubMed1988

The study of thermal and gross morphologic properties of polyglycolic acid upon annealing and degradation treatments.

The objective of this study is to alter the fiber morphology of a linear aliphatic polyester, polyglycolic acid, by annealing treatment and to examine the changes of its degradation properties. The annealing was done at 150 degrees C, 170 degrees C, and 190 degrees C, and the specimens were annealed in four different strained conditions, freely hung, 0, 1, and 10%. After annealing treatments, the specimens were subject to in vitro hydrolytic degradation by immersing them in phosphate-buffer solution of pH 7.4 at 37 degrees C for up to 28 days. The thermal properties and gross morphology of the specimens were obtained. It was found that annealing treatments resulted in initial higher levels of crystallinity, which, in turn, influenced the hydrolytic degradation of the fiber. Among all the annealing conditions, the freely hung specimens annealed at 190 degrees C exhibited the most pronounced annealing effect on hydrolytic degradation, and was consistent with the observed gross morphologic changes. The change in the characteristics of fiber structure (i.e., the return to the stage of less oriented conformation upon freely hung annealing) was thought to be the cause.

Biocompatible Materials↗
PubMed1977

Failure of lactoperoxidase to iodinate specifically the plasma membrane of cucurbita tissue segments.

An attempt has been made to use lactoperoxidase-catalyzed iodination of excised Cucurbita hypocotyl hooks to monitor the distribution of plasma membrane fragments relative to that of phytochrome in particulate fractions from this tissue. Upon fractionation, the iodinated tissue yields a 20,000g pellet which contains 58% of the trichloroacetic acid-precipitable (125)I at a specific radioactivity 12 times that of the proteins in the supernatant. On sucrose gradients, the labeled fraction has a mean isopycnic density of 1.15 g . cm(-3). The distribution profile is distinct from that of the total particulate protein and does not coincide with either mitochondrial or endoplasmic reticulum markers. These observations satisfy operational criteria commonly accepted in other systems as indices of selective labeling of the cell surface. The sucrose gradient profiles of the phytochrome and (125)I in the 20,000g pellets are noncoincident. In the absence of more direct evidence, this is readily interpreted to indicate a lack of association of the pigment with the plasma membrane.Autoradiographic analysis indicates, however, that the (125)I is almost exclusively associated with an amorphous film (possibly phloem-exudate protein) overlying the cut cells at the point of prelabeling excision and along the outer physical surface of the hypocotyl cuticle. No evidence of plasma membrane labeling is apparent. The observed membrane-like behavior of the iodinated material upon cell fractionation is attributed to the preferential posthomogenization association of this material with a particular membrane fraction(s). These data indicate that in addition to the well recognized potential for spurious labeling of the internal cytoplasmic proteins of leaky cells, a further source of ambiguity in surface-labeling experiments should be considered. That is, the potential for labeling extracellular proteins of nonplasma membrane origin but with a capacity to become associated with membranes upon homogenization.

Journal Article↗
PubMedDate not supplied

The effect of annealing treatments on the tensile properties and hydrolytic degradative properties of polyglycolic acid sutures.

The purpose of this research was to examine the effect of annealing treatments on the mechanical properties of polyglycolic acid sutures, and their subsequent influence on PGA degradation properties. An attempt was made to develop a better understanding of the degradation mechanism of synthetic absorbable sutures, including the relationship of their structure, morphology, and mechanical properties. PGA sutures were annealed under selected axial strain (freely hung, 0%, 1%, 10%), at four temperatures (150 degrees C, 170 degrees C, 180 degrees C, 190 degrees C), and two times (5 and 20 min). The annealed PGA specimens were then subjected to hydrolysis in phosphate-buffer solutions (pH = 7.4) for up to 28 days at 37 degrees C. Tensile properties were used to evaluate the effect of annealing treatments. The data were subjected to statistical analysis using the SAS system. All of the one-, and many of the two- and three-factor interactions were found to be statistically significant. Annealing treatments did alter the mechanical properties of PGA sutures, as well as their degradation properties. Except for the increase in tenacity of samples with increasing percent extension, all other respects of the annealing treatments resulted in lower tenacity and breaking elongation when compared with the control samples. Sutures that have been exposed to any level of axial tension during annealing, however, exhibited a lower rate of hydrolytic degradation than the freely hung suture samples. The reduction of the characteristics of fiber structure due to the tendency of the tie-chain molecules to acquire the less constrained conformations and thus to bring the crystal blocks they connect back to the original arrangement before drawing is believed to be responsible for the freely hung specimens to behave quite differently from the clamped and stressed PGA samples.

Hydrolysis↗