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A Brunot

Publications and source records attributed to A Brunot.

6 recordsLinked to original sources
PubMed1996

Coupling of MALDI-TOF mass analysis to the separation of biotinylated peptides by magnetic streptavidin beads.

Extraction of biotinylated peptides by streptavidin magnetic beads has been directly coupled to the MALDI-TOF mass analysis. The elution of peptides from the beads is achieved by first mixing the beads with the MALDI matrix solution and removing, after a few minutes, the beads with a magnet; then, the matrix solution containing the biotinylated peptide is directly mass analyzed by MALDI. Three examples are presented to show the capabilities of this procedure to detect biotinylated peptides present at very low concentrations in complex mixtures. Detection limits of less than 100 finol can be achieved. Such a coupling strategy is of great interest to investigate peptide/ protein interactions.

Amino Acid Sequence↗
PubMed1993

Failure to detect spindle-shaped fibroblastoid cell progenitors in PBPC collections.

We assessed the development of an adherent stromal layer in peripheral blood progenitor cells (PBPC) collections of 17 patients with solid tumors and hematological malignancies (n = 38), as well as in normal and disease-free bone marrow samples (n = 14). Light-density mononuclear cells from peripheral blood leukapheresis maintained in long term culture (LTC) conditions for at least 3 weeks failed to develop a stromal layer as was observed in all bone marrow samples. We found no differences with regard to CFU-GM growth, CD34+ percentage or cytological morphology. Our results indicate that spindle-shaped fibroblastoid cell progenitors present in the bone marrow are not mobilized towards peripheral blood by chemotherapy and/or growth factor stimulation, contrasting with hematopoietic progenitors, which, as is well known, are able to circulate. The absence of stromal progenitors in PBPC autografts does not appear to be relevant for a successful hematopoietic engraftment.

Cell Adhesion↗