Occurrence of fumonisin B1 and hydrolyzed fumonisin B1 in Mexican nixtamalized cornmeal.
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Carcinogens, Environmental↗
Biomedical subjects
A Burgos-Hernández
Publications and source records attributed to A Burgos-Hernández.
Detection and quantification of insecticides in shrimp grown in a coastal farm in Sonora, Mexico.
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Animals↗
Partial chemical/structural elucidation of anti-mutagenic compounds from corn.
In this study, corn fractions obtained from an isolation process of anti-mutagenic factors in our previous research work (Burgos-Hernández et al., 2001), were subjected to several analyses for chemical/structural elucidation. The anti-mutagenic activity of these fractions was tested against aflatoxin B(1) (AFB(1)) and 1-methyl-3-nitro-1-nitrosoguanidine (MNNG), a mutagen that does not require bioactivation. Two concentrations of this agent in the corn fractions were tested for anti-mutagenicity in the Salmonella/microsomal mutagenicity assay, using tester strain TA100 with no metabolic activation. Corn fractions tested showed evidence of anti-mutagenic activity by producing a dose-response type of relationship between a constant amount of MNNG and several concentrations of tested corn fraction. Five different varieties of yellow corn were tested in order to determine if the anti-mutagenic factors were intrinsic to corn. Variety of the corn did not show an effect on the reduction of the mutagenic potential of AFB(1) suggesting that anti-mutagenic compounds are intrinsic to corn. Four corn fractions, previously obtained after the isolation process were analyzed by MALDI-MS and GC-MS. MALDI-MS showed the presence of two groups of molecules or molecular fragments. The molecular mass of one group ranged from 250 to 370 m/z, the other ranged from 540 to 640 m/z. GC-MS identified linoleic acid as one of the compounds responsible for the anti-mutagenic activity present in corn.
Aflatoxin B1↗
Anti-mutagenic compounds from corn.
In previous studies with aflatoxin-contaminated corn an uncharacteristic response for AFB1 in the Salmonella/microsomal mutagenicity assay (Ames test) was observed and the presence of anti-aflatoxin factors in the corn was suggested. In the current study, corn was extracted and fractionated using thin layer chromatography (TLC) using different developing solvent systems and the Ames test was used to monitor for anti-mutagenic activity in the corn fractions. Both Salmonella tester strains TA98 and TA100 with metabolic activation (S9) were used. Several corn fractions, at different stages in the isolation and purification process, showed anti-mutagenic dose-responses when exposed to pure AFB1. Corti extracts were non-toxic to the tester strains and TLC fractions that showed the best anti-mutagenic dose-responses were selected for further partial characterization analyses.
Aflatoxin B1↗