PubMed Health⌕ Search

Biomedical subjects

A Butterworth

Publications and source records attributed to A Butterworth.

At least 19 recordsLinked to original sources
PubMed2006

Investigation into possible causes of interference in serum testosterone measurement in women.

BACKGROUND: Direct (non-extracted) testosterone immunoassays may give spuriously high results in women. The presumed interferents may be removed if testosterone is extracted into an organic phase before being measured. We aimed to clarify possible causes and effects of interference in testosterone measurement in women. METHODS: Women who had a blood sample referred to Hope Hospital Clinical Biochemistry laboratory for measurement of serum testosterone concentration over a six-month period were studied. Clinical and treatment data were recorded. A difference (direct-minus-extracted testosterone) of less than 1.0 nmol/L was used to define a group with low interference. A difference of 2.5 nmol/L or more was used to define a group with significant interference. RESULTS: The distribution of interference in 1271 serum samples from female patients was unimodal. There were no group differences in clinical presentation or treatment. The median degree of interference was 1.4 nmol/L. In 42 female patients with varying degrees of interference, identified on routine assay, regression analysis showed strong association between dehydroepiandrosterone sulphate (DHEA-S) and interference (direct-minus-extracted testosterone) (r = 0.77, P < 0.001). CONCLUSIONS: Given that DHEA-S is present in very variable amounts in blood, it is possible that even with a low cross reactivity, DHEA-S or one of its metabolites significantly interferes in the testosterone assay when at higher concentrations.

Adult↗
PubMed2005

Estimate of radiation damage to low-level electronics of the RF system in the LHC cavities arising from beam gas collisions.

Monte Carlo simulations have been performed to estimate the radiation damage induced by high-energy hadrons in the digital electronics of the RF low-level systems in the LHC cavities. High-energy hadrons are generated when the proton beams interact with the residual gas. The contributions from various elements-vacuum chambers, cryogenic cavities, wideband pickups and cryomodule beam tubes-have been considered individually, with each contribution depending on the gas composition and density. The probability of displacement damage and single event effects (mainly single event upsets) is derived for the LHC start-up conditions.

Computer Simulation↗
PubMed2004

Evaluation of baseline indices of sensibility in captive cetaceans.

Twelve possible tests of sensibility and six possible tests of vitality were evaluated for their ease of application and the reliability of the animals' responses in 25 animals of six species of captive cetaceans. The protocols for the application of the tests and the responses observed are described.

Animals↗
PubMed2003

A comparison of direct microscopy, the concentration method and the Mycobacteria Growth Indicator Tube for the examination of sputum for acid-fast bacilli.

SETTING: In countries with high human immunodeficiency virus prevalence, laboratory diagnosis of pulmonary tuberculosis with the standard Ziehl-Neelsen (ZN) technique is characterised by low sensitivity. OBJECTIVE: To compare test characteristics of direct microscopy, the concentration method and the Mycobacteria Growth Indicator Tube (MGIT). DESIGN: Three hundred specimens from patients diagnosed with pulmonary tuberculosis were tested for the presence of mycobacteria. Specimens were stained with ZN, decontaminated by adding 4% NaOH, concentrated by centrifuging and processed in MGIT broth. The gold standard was defined as a positive MGIT culture or a positive acid-fast bacilli smear of material obtained from a negative culture after 42 days. RESULTS: A total of 44 (14.7%) specimens were contaminated. Of 256 valid specimens, 234 (91.4%) were positive according to the gold standard definition. Decontamination and concentration of the sample increased the sensitivity of direct microscopy from 67.5% to 87.1%. Specificity remained unchanged (95.5%). The overall median time to detection of MGIT culture-positive specimens was 5 days, ranging from 4 (direct smear-positive specimens) to 12 days (concentration smear-negative specimens). CONCLUSION: The concentration method substantially increases the sensitivity of direct microscopy without much extra input. The MGIT culture technique has considerable advantages, but its relatively high contamination rate and its high cost make it a less recommendable option for widespread use in routine district laboratories.

Bacteriological Techniques↗
PubMed2001

Molecular typing of strains of staphylococcus aureus isolated from bone and joint lesions in lame broilers by random amplification of polymorphic DNA.

A study was conducted to investigate the relationship between the presence of localized bacterial infection and lameness in broiler fowl (Gallus gallus domesticus). Isolation of bacteria from the proximal femur, proximal tibia, and tibiotarsus from broilers with lameness revealed a probable association between lameness and the presence of Staphlylococcus aureus. Other potential pathogens, including Escherichia coli and DNase-negative staphylococci, were also isolated from sound and lame birds, and their association with pathologies causing lameness was less well defined. After trials with a set of twenty 10-base oligonucleotide primers, a pair of primers giving optimal performance was selected. The S. aureus isolates were typed by random amplification of polymorphic DNA (RAPD) by using the pair of 10-mer primers, and groupings were defined by banding patterns after agarose gel electrophoresis. The putative RAPD groupings may provide a basis for epidemiological studies of S. aureus in broiler production systems.

Animals↗
PubMed2000

Changes in the blood biochemical and haematological profile of neonatal calves with age.

Fourteen calves were used to investigate the changes from birth to 83 days of age in the concentrations of serum albumin, alkaline phosphatase, beta-hydroxybutyrate, plasma cortisol, serum creatine kinase, creatinine, iron, plasma fibrinogen, serum gamma-glutamyl transferase, plasma glucose, haptoglobin, serum non-esterified fatty acids, total protein, transferrin, triglycerides, urea and gamma globulin; the haematological variables measured were: basophils, eosinophils, haematocrit, haemoglobin, lymphocytes, mean cell haemoglobin, mean cell haemoglobin concentration, mean cell volume, monocytes, band neutrophils, neutrophils, platelets, red blood cells and white blood cells. The changes are presented as a series of graphs and the values are discussed in relation to the published reference ranges for adult cattle. Two populations of calves were identified which gave rise to a bimodal distribution for some of the variables. Differences in haematocrit, haemoglobin and red blood cell counts were apparent at birth, with raised values for these measurements being associated with an increased white blood cell and neutrophil count between three and 27 days of age.

Aging↗
PubMed1994

The outcome of training community psychiatric nurses to deliver psychosocial intervention.

BACKGROUND: A prospective quasi-experimental design was used to evaluate the effect of training CPNs to undertake psychosocial intervention with families caring for a relative with schizophrenia. METHOD: Patients meeting predetermined criteria were allocated to either an initial waiting-list control group or a delayed intervention group. The CPNs were trained to offer family interventions to a total target group of 48. Treatment was eventually accepted by 85%, of whom 81% (n = 34) were followed-up for 12 months. Families received a weekly mean session of 47 minutes (compared with 33 minutes in the controls). The main outcome measures are frequency and severity of symptoms (KGV), social functioning (SFS) and days in hospital. Carers' minor psychiatric morbidity (GHQ) and knowledge (KASI) are also reported. RESULTS: In the patient group both the positive and negative symptoms of schizophrenia improved significantly (P < or = 0.001 and P < or = 0.01 respectively) to 12-month follow-up, as did a global measure of social functioning (P < or = 0.001). Tentative evidence was also collected that family intervention reduced in-patient episodes. Benefits for relatives included a decrease in minor psychiatric morbidity (P < or = 0.05) and an increase in knowledge about neuroleptic drugs (P < or = 0.001). CONCLUSIONS: The study offers some evidence that CPNs can be taught to improve the outcome for families who care for a relative with schizophrenia.

Activities of Daily Living↗
PubMed1993

Oil on Shetland.

Explore the source record for details and available documents.

Accidents↗
PubMed1992

Training community psychiatric nurses for psychosocial intervention. Report of a pilot study.

Community psychiatric nurses were trained to deliver psychosocial intervention to clients with a diagnosis of schizophrenia living at home with relatives. The training package consisted of family assessment, health education, and family stress management strategies. In a 'quasi-experimental' design, a sample of families (n = 54) were recruited to either a control or experimental condition and followed up for 12 months. A number of favourable outcomes were observed in the experimental group, including improvements in the client's target symptoms, personal functioning, and social adjustment. Relatives' satisfaction with services increased and reports of their own minor psychiatric morbidity improved.

Adolescent↗
PubMed1989

Cytotoxic effects in vitro of human monocytes and macrophages on schistosomula of Schistosoma mansoni.

Human peripheral blood monocytes from normal donors were isolated by differential centrifugation and cultured in vitro in hydrophobic Teflon-coated tissue culture bags. Cells were harvested between 0 and 10 days and tested for their ability to kill schistosomula of Schistosoma mansoni in an in-vitro cytotoxicity assay. Freshly isolated, unstimulated monocytes demonstrated minimal cytotoxic capability. However, this was increased if the cells were pretreated with human recombinant gamma interferon (IFN-gamma), or with specific anti-S. mansoni antiserum. As the monocytes matured in vitro there were marked increases in the levels of antibody-independent killing of schistosomula. Monocytes grown in vitro with IFN-gamma (10(4) u/ml) took 2-3 days to develop almost maximal cytotoxicity (mean 94% kill of schistosomula). In contrast, unstimulated monocytes (no IFN-gamma) took between 5 and 7 days to achieve comparable cytotoxicity (mean 99% kill). Killing of the schistosomula was dependent upon a high effector to target ratio, and was a relatively slow phenomenon in vitro, parasite attrition occurring between 17 and 36 h. Supernatants from cytotoxic macrophages were ineffective in mediating cytotoxicity of the parasite.

Adult↗
PubMed1988

Immunity in human schistosomiasis mansoni: cross-reactive IgM and IgG2 anti-carbohydrate antibodies block the expression of immunity.

We have previously reported that the slow development of immunity to reinfection after treatment of Schistosoma mansoni infections is partly attributable to the continued presence of 'blocking' antibodies in young, susceptible children. A further analysis of this phenomenon supports the hypothesis that such blocking antibodies can be of the IgG2 as well as the IgM isotype, and that they react with carbohydrate epitopes expressed both on egg polysaccharides and on schistosomulum surface antigens, of particular importance being those antigens that are shed from the schistosomulum surface during the early stages of maturation in vitro. Evidence is also presented that, in those patients lacking high levels of IgG2 blocking antibodies, resistance to reinfection after treatment is associated with the presence of other IgG isotypes against the same shed antigens.

Animals↗
PubMed1987

Fetal kidney size related to gestational age.

Fetal renal anatomy was assessed in 660 apparently normal fetuses. The problems of technique of visualization of the kidneys are outlined. A correlation of fetal kidney length and gestational age is presented. The results are presented to be used as an adjunct in establishing fetal gestational age, as well as the assessment of normal renal morphology in routine obstetric ultrasound assessment.

Embryonic and Fetal Development↗
PubMed1987

A carbon chamber for vital microscopy of bone healing.

We describe a carbon chamber implanted in the ulna of fowl. A window permitted daily microscopy of bone formation including observations of tetracyline labelling. The chamber could be heated to 5 degrees above body temperature by 600 kHz radio frequency.

Animals↗
PubMed1984

In utero diagnosis of a vein of Galen aneurysm by ultrasound.

A large midline supratentorial cystic lesion with associated cardiomegaly was seen in utero by ultrasound during the 34th week of pregnancy. The provisional diagnosis of a vein of Galen aneurysm was confirmed with CT and angiography after birth.

Adult↗
PubMed1983

Splenic abscess: a review with the value of ultrasound.

Five cases of splenic abscess are presented. A positive ultrasound diagnosis was made in each patient, showing a regular or ill-defined anechoic mass with large or small, high-intensity echogenic foci due to contained debris. Computed tomography performed in two cases showed intrasplenic low-density areas essentially unchanged following intravenous contrast administration. Gas was present in one of the abscesses. The clinical implications in a septicaemic patient with intrasplenic gas formation following splenic embolisation are discussed.

Abscess↗
PubMed1980

Ultrasound and barium study in the evaluation of upper abdominal masses.

Numerous radiological procedures are now available for investigation of upper abdominal masses of uncertain aetiology; however, certain limitations and pitfalls are associated with these procedures. It is suggested that the initial use of ultrasound and barium studies would frequently provide either a definite diagnosis or lead to the next appropriate line of investigation. The importance of not omitting the barium study is stressed.

Abdominal Neoplasms↗