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Biomedical subjects

A C Alessi

Publications and source records attributed to A C Alessi.

7 recordsLinked to original sources
PubMed2002

Experimental Trypanosoma evansi infection in South American coati (Nasua nasua): hematological, biochemical and histopathological changes.

The course of an experimental Trypanosoma evansi infection in coatis (Nasua nasua, carnivora, Procyonidae) was followed for 262 days. Hematological analysis of the infected coatis revealed a marked decline in hemoglobin, packed-cell volume, and total erythrocyte count. An intense anemia followed the first wave of parasitemia and persisted until the end of the experimental period. Biochemical analysis showed increased serum levels of alanine aminotransferase and aspartate aminotransferase and decreased albumin. The main histopathological features consisted of myocarditis with the presence of degenerate cardiac fibers and meningoencephalitis. This study has shown that coatis infected with T. evansi develop a chronic disease.

Alanine Transaminase↗
PubMed1994

Toxicologic evaluation of acute and subacute oral administration of Cucurbita maxima seed extracts to rats and swine.

The extract prepared from dried seeds of Cucurbita maxima was administered to rats and pigs. Following a single dose or 4 weeks of daily oral administration, the extract produced no changes in serum glucose, urea, creatinine, total protein, uric acid, GOT, GPT, LDH or blood counts. Urine analysis (urea, uric acid, creatinine, total protein, Na and K), as well as histopathological investigation, showed no abnormalities. These results taken as a whole indicate that the seeds of C. maxima as used in Brazilian folk medicine are not toxic for rats and swine.

Administration, Oral↗
PubMed1994

Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.

1. We describe the isolation of viable merozoites from erythrocytes infected with Babesia bovis or Babesia bigemina organisms by ammonium chloride lysis. 2. Parasite morphology was examined by both light and transmission electron microscopy. Erythrocyte-free parasites maintain their viability and infectivity, retain their antigenicity and are suitable for use in the indirect fluorescent antibody assay.

Ammonium Chloride↗
PubMed1990

Immunosuppression by peritoneal BCG-induced granulomas in mice.

1. Intraperitoneal injection of viable BCG resulted in the formation of peritoneal granulomas in mice. Granuloma-bearing animals presented a specific immunosuppression of delayed-type hypersensitivity (DTH) to BCG. 2. The transplantation of peritoneal granulomas induced by BCG to animals previously sensitized with BCG suppressed the DTH response to this antigen in the recipient animals to the same extent as observed in animals with granulomas induced by the injection of BCG. No suppression was observed in animals transplanted with granulomas induced by either Cytodex or talc. 3. The hypersensitivity reaction to Brucella abortus and the acute inflammatory reaction produced by carrageenin were not affected by granuloma transplantation. 4. Granulomas collected from animals treated with a lethal does of irradiation maintained the capacity to suppress DTH in recipient animals. 5. These results suggest that the response observed in granuloma recipients is modulated by cells within the granuloma.

Animals↗
PubMedDate not supplied

Canine lymphomas: a morphological and immunohistochemical study of 55 cases, with observations on p53 immunoexpression.

The purpose was to determine the immunophenotype of canine lymphomas (CLs) classified according to the WHO nomenclature for domestic animals, and to relate these findings to the immunoexpression of p53 protein. Lymphomas were immunophenotyped with antibodies to CD79a, CD3, and p53 protein, suitable for paraffin wax-embedded tissue sections. Of 55 cases, 40 (72.7%) were of the B-cell phenotype, 12 (21.8%) of the T-cell phenotype, and three (5.4%) were non-B-non-T lymphomas. Of the 40 B-cell lymphomas, 31 were of the large B-cell type, six were lymphoplasmacytic, one lymphocytic, one follicular (grade II) and one unclassified low-grade. One of the peripheral T-cell lymphomas was morphologically consistent with anaplastic large cell lymphoma. Immunolabelling for p53 occurred in 24 B-cell and seven T-cell lymphomas. Lymphomas with high grade histology showed a significantly increased frequency of p53 positivity (P = 0.01). Positivity for p53 (more than 10% positive cells) tended to be associated with the T-cell phenotype (P = 0.06). Mean patient age was significantly higher in p53-positive cases (P = 0.02). These data are comparable with findings in human lymphomas.

Animals↗
PubMedDate not supplied

Clinical, parasitological and immunological aspects of experimental infection with Trypanosoma evansi in dogs.

This research investigated the pattern of antibody response by means of enzyme linked immunosorbent assay (Elisa) and indirect fluorescent antibody test (IFAT) through the course of experimental Trypanosoma evansi infection in dogs. Clinical and parasitological features were also studied. The average prepatent period was 11.2 days and parasitaemia showed an undulating course. Biometrical study of parasites revealed a mean total length of 21.68mm. The disease was characterized by intermittent fever closely related to the degree of parasitaemia and main clinical signs consisted of pallor of mucous membrane, edema, progressive emaciation and enlargement of palpable lymph nodes. Diagnostic antibody was detected within 12 to 15 days and 15 to 19 days of infection by IFAT and Elisa, respectively. High and persistent antibody levels were detected by both tests and appeared not to correlate with control of parasitaemia

Animals↗