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A C Borges

Publications and source records attributed to A C Borges.

66 records · Page 4Linked to original sources
PubMed1992

Coordinate pretranslational control of cAMP-dependent protein kinase subunit expression during development in the water mold Blastocladiella emersonii.

The aquatic fungus Blastocladiella emersonii provides a system for studying the regulation of expression of regulatory (R) and catalytic (C) subunits of cAMP-dependent protein kinase (PKA). Blastocladiella cells contain a single PKA with properties very similar to type II kinases of mammalian tissues. During development cAMP-dependent protein kinase activity and its associated cAMP-binding activity change drastically. We have previously shown that the increase in cAMP-binding activity during sporulation is due to de novo synthesis of R subunit and to an increase in the translatable mRNA coding for R (Marques et al., Eur. J. Biochem. 178, 803, 1989). In the present work we have continued these studies to investigate the mechanism by which the changes in the level of kinase activity take place. The C subunit of Blastocladiella has been purified; antiserum has been raised against it and used to determine amounts of C subunit throughout the fungus' life cycle. A sharp increase in C subunit content occurs during sporulation and peaks at the zoospore stage. Northern blot analyses, using Blastocladiella C and R cDNA probes, have shown that the levels of C and R mRNAs parallel their intracellular protein concentrations. These results indicate a coordinate pretranslational control for C and R subunit expression during differentiation in Blastocladiella.

Blastocladiella↗
PubMed1987

[Sacral agenesis associated with a teratoma].

A case of a 29-year-old male with a huge sacrococcygeal teratoma completely removed by surgery, and associated with a sacral agenesis is reported. Roentgenograms revealed fusion of the T12, L1, L2, and L3 vertebral bodies, absence of the left 12th rib, partial agenesis of sacrococcygeal elements and a lumbar scoliosis convex to the left. Cystometry and myelograms were normal. No such association has been found in the literature reviewed. Some topics on the embryology of these lesions are discussed.

Adult↗
PubMed1982

[Acute pulmonary edema secondary to a posterior fossa tumor: report of a case].

A case of a 44-year-old woman presenting pulmonary edema secondary to posterior fossa meningioma is reported. The authors have tried to demonstrate the relationship between a lesion occupying space in the posterior fossa and important pulmonary abnormality such as interstitial edema based on increasing A-aDO2 (oxygen alveolar arterial gradient) in this peculiar case. A-aDO2 is easily measured and represents a good guide for evaluating the efficiency of medical and neurosurgical management when it is done in the serial way, particularly in those cases of non cardiogenic pulmonary edema.

Acute Disease↗
PubMed1980

[Guillain-Barré syndrome simulating hernia of lumbar disc. Report of a case].

The case of a 52 years-old man, with typical findings of lumbar disc disease is reported. The radiological investigation was negative and albuminocytologic dissociation in cerebrospinal fluid was detected. The clinical course was similar to Guillain-Barré syndrome which was completely solved by treatment with steroids. Brief comments on clinical aspects of that syndrome are made.

Diagnosis, Differential↗
PubMed1980

[Plantar ulcer secondary to spondylolisthesis. Report of a case].

A case of a 41 year-old-woman with a long history of back pain and bilateral plantar ulcer due to spondylolisthesis is reported. Symptomatology was solved by decompression followed by spinal fusion. Brief comments are presented on clinical aspects and surgical treatment.

Adult↗
PubMed1977

[Subdural abscess at the level of the cauda equina; report of a case].

A case of staphylococcal subdural abscess at cauda equina level region in a 42 year old male patient is reported. The neurological signs presented in this condition are discussed briefly and it is pointed out the importance of laminectomy associated to antibiotic treatment to achieve good outcome.

Abscess↗
PubMedDate not supplied

Enteric bacterial catalysts for fuel ethanol production.

The technology is available to produce fuel ethanol from renewable lignocellulosic biomass. The current challenge is to assemble the various process options into a commercial venture and begin the task of incremental improvement. Current process designs for lignocellulose are far more complex than grain to ethanol processes. This complexity results in part from the complexity of the substrate and the biological limitations of the catalyst. Our work at the University of Florida has focused primarily on the genetic engineering of Enteric bacteria using genes encoding Zymomonas mobilis pyruvate decarboxylase and alcohol dehydrogenase. These two genes have been assembled into a portable ethanol production cassette, the PET operon, and integrated into the chromosome of Escherichia coli B for use with hemicellulose-derived syrups. The resulting strain, KO11, produces ethanol efficiently from all hexose and pentose sugars present in the polymers of hemicellulose. By using the same approach, we integrated the PET operon into the chromosome of Klebsiella oxytoca to produce strain P2 for use in the simultaneous saccharification and fermentation (SSF) process for cellulose. Strain P2 has the native ability to ferment cellobiose and cellotriose, eliminating the need for one class of cellulase enzymes. Recently, the ability to produce and secrete high levels of endoglucanase has also been added to strain P2, further reducing the requirement for fungal cellulase. The general approach for the genetic engineering of new biocatalysts using the PET operon has been most successful with Enteric bacteria but was also extended to Gram positive bacteria, which have other useful traits for lignocellulose conversion. Many opportunities remain for further improvements in these biocatalysts as we proceed toward the development of single organisms that can be used for the efficient fermentation of both hemicellulosic and cellulosic substrates.

Alcohol Dehydrogenase↗