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Biomedical subjects

A C McCaskill

Publications and source records attributed to A C McCaskill.

5 recordsLinked to original sources
PubMed1991

A specific, sensitive and high-capacity immunoassay for PAF.

A specific radioimmunoassay for platelet-activating factor (PAF) sensitive in the range 10-1000 pg (0.02-2 pmoles) has been developed. Detailed quantitative hapten inhibition studies showed specificity for the acetyl group at C-2 of PAF, a requirement for the ether linkage at C-1 and some tolerance for substituents on the choline nitrogen. No significant cross-reactivity was found with phosphatidylcholine and lysophosphatidylcholine or with lysoPAF.

Animals↗
PubMed1984

Anaphylaxis following intranasal challenge of mice sensitized with ovalbumin.

Mice sensitized with two intraperitoneal injections of ovalbumin and challenged intranasally with the same antigen developed a non-fatal anaphylactic shock peaking in severity 30 min after challenge. Increases in haematocrit were noted which corresponded to the severity of signs of shock displayed by mice. Severity of shock also correlated with IgE and IgG levels. Sensitization by the nasal route, and use of B. pertussis vaccine as adjuvant had no qualitative effect upon the response. Cobra venom factor depletion of C3 in vivo did not alter the response of mice, which suggests anaphylaxis did not involve complement activation. Sensitivity was not transferrable to non-immune mice with serum. Passive sensitization with polyclonal and monoclonal antibodies produced inconsistent results. Possible mechanisms of anaphylaxis are discussed.

Administration, Intranasal↗
PubMed1982

IgE and haemagglutinating antibody production in mice following intranasal immunization with ryegrass pollen.

Repeated intranasal immunization of mice whole ryegrass pollen (RGP) led to the development of high circulating titres of both IgE and haemagglutinating antibodies. Bordetella pertussis vaccine (BPV) was required at the time of initial immunization, although its repeated use did not affect IgE production. Antibody production was maintained at high levels for greater than 4 months. Significant differences were found in the ability of different batches and strains of BPV to induce production.

Adjuvants, Immunologic↗
PubMed1981

Enrichment and expansion of specific antibody-forming cells by adoptive transfer and clustering, and their use in hybridoma production.

Adoptive transfer regimens have been examined as a method of enriching and expanding antibody-forming cells (AFC). When spleens from mice which had reverted to memory or from those at the peak of an AFC response were transferred to syngeneic irradiated recipients, a comparable enrichment in AFC of about 10-fold was found. However, recently re-stimulated spleen cells gave much better expansion of total AFC in the recipient mice. The degree of expansion was examined using different routes and timing of antigen stimulus and AFC recovery. With the optimum protocol found the AFC pool obtained from adoptively-transferred recipients was on average 80-fold greater than from conventionally re-immunised mice in a number of experiments. Further enrichment of the AFC was shown by an in vitro clustering technique which gave suspensions with AFC enriched to better than 1 cell in 10. Cluster-enriched and adoptive-transfer enriched populations were both shown to give a much higher incidence of successful specific hybridoma production than spleen cells from conventionally re-immunised mice.

Animals↗