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Biomedical subjects

A C Rodloff

Publications and source records attributed to A C Rodloff.

63 records · Page 4Linked to original sources
PubMed1984

Synergistic lethality in experimental infections with Escherichia coli and Bacteroides fragilis.

Using an animal model, a synergism in lethality of infections with Escherichia coli and Bacteroides fragilis is described. This synergism was dependent on certain dosage combinations of the infectious agents. It was demonstrated that animal-passaged, laboratory-passaged, and heat-killed B. fragilis alike were capable of producing this synergism. However, animal-passaged B. fragilis showed superior recovery rates from animal organs.

Animals↗
PubMed1983

Intravenous injection of mice with bacteroides fragilis and Escherichia coli: role of Thioglycollate medium in the infectious process.

Mice were intravenously infected with E. coli, B. fragilis, and combinations of both, and the bacterial contents of livers, kidneys, and spleens were monitored from the first up to the 9th day postinfection. If Thioglycollate medium was added to otherwise sublethal doses of E. coli, the inoculum was able to induce severe and for some animals lethal infections. B. fragilis was not capable to produce prolonged infections; however, when given together with E. coli and Thioglycollate medium, both bacteria were recovered from some animal organs even on day 9 postinfection. Employing this experimental model of a mixed anaerobic/facultative bacterial infection, previous reports attributing animal lethality to E. coli were confirmed, but a synergism between E. coli and B. fragilis could not be demonstrated.

Animals↗
PubMed1981

[Lesions in the central nervous system of the mouse caused by yellow fever 17 D. An animal model of the histological evaluation of viral encephalitis (author's transl)].

Random-Bred-Swiss Mice were inoculated intracerebrally with 0.02 ml of a 10(-1) diluted suspension of yellow fever virus 17 D. The animals were sacrificed at selected times ranging from 1 day up to 168 days after inoculation. Brain sections were stained and then histologically investigated. Nerve cell necrosis in the cornu ammonis could be seen already 24 h after inoculation, again 24 h later inflammatory signs were found. There was no spatial correlation between nerve cell necrosis and inflammation.--Although the animals did not show clinical signs of infection for more than 2 weeks, nerve cell necrosis was still progressive, even in mice sacrificed 168 days after inoculation of the virus.--The implications of those findings are discussed and the definition of encephalitis established by Spatz is challenged.--The above described approach may also serve as a model for explaining etiological findings with regard to the pathogenesis of degenerative diseases of the central nerve system.

Animals↗
PubMedDate not supplied

Combined activity of trospectomycin and colloidal bismuth subcitrate against Helicobacter pylori in vitro.

The combined activities of trospectomycin, a spectinomycin analogue, and colloidal bismuth subcitrate against Helicobacter pylori were investigated, the agar dilution method being employed. Forty-seven strains of H. pylori were examined. An additive effect was observed in 89%, and a synergistic interaction in 11% of the isolates. There was no antagonism observed. The MIC50 of trospectomycin against H. pylori was 2 mg/l; the MIC ranged from 0.5 to 4 mg/l.

Antacids↗
PubMedDate not supplied

Induction of an atypical interferon by bacteroides fragilis and Escherichia coli in experimental infections and in leukocyte cultures.

Previous reports have shown that Bacteroides fragilis may enhance the pathogenicity of coinfecting enterobacteriaceae by interfering with the host's immune response. With the present study, we have investigated the possible role of interferons (IFN) in mediating these effects. Mice injected with B. fragilis developed moderate serum levels of IFN that appeared just prior to alterations of the animals' immunity described earlier. The IFN was neutralized by treatment with anti-IFN-alpha/beta-antibodies or hydrochloric acid; hence it displayed the same "atypical" characteristics as IFN found in patients with immuno-compromising diseases such as AIDS, systemic lupus erythematosus or rheumatoid arthritis. Escherichia coli displayed the same induction patterns as B. fragilis, while gram-positive bacteria induced "regular" IFN alpha/beta and gamma. Spleen cells, peritoneal macrophages, or liver leukocytes taken from B. fragilis or E. coli-injected animals 6 h post infection were refractory to IFN induction by E. coli lipopolysaccharide in vitro; cells from mice infected with gram-positive organisms showed normal or enhanced responsiveness.

Animals↗
PubMedDate not supplied

Identification and susceptibility patterns of anaerobic bacteria isolated from clinical specimens during a one-year period.

During the whole of 1983, all anaerobes cultured from clinical specimens were identified using both the Minitek system and gas chromatography. Antimicrobial susceptibility patterns were established using a broth microdilution test. The results show isolation rates for the individual species to be in good agreement with previous studies. Despite widespread use (even for prophylactic purposes), mezlocillin, latamoxef, cefoxitin, metronidazole and clindamycin retained their high activity against anaerobic bacteria and no clusters of resistance were observed. Augmentan also showed excellent in vitro activity. Penicillin was found to be inactive against most gram-negative anaerobic rods while tetracycline showed a rather high degree of resistance.

Anti-Bacterial Agents↗
PubMedDate not supplied

Pericarditis after allogeneic peripheral blood stem cell transplantation caused by Legionella pneumophila (non-serogroup 1).

A case of Legionella pericarditis caused by a Legionella pneumophila isolate other than serogroup 1 is reported in a 59-year-old man after allogeneic peripheral blood stem cell transplantation. On admission a 5 mm pericardial effusion was detected on echocardiography. Antibodies were detected against L. pneumophila serogroups 7 to 14 using the antigen pool and against serogroup 12 alone. Antibodies were not detected against the serogroup 1 to 6 antigen pool. The patient's clinical condition improved dramatically after treatment with clarithromycin and an echocardiography revealed the total disappearance of the pericardial effusion.

Anti-Bacterial Agents↗