PubMed Health⌕ Search

Biomedical subjects

A C Skinner

Publications and source records attributed to A C Skinner.

8 recordsLinked to original sources
PubMed1995

Extensive polymorphism at the Gp63 locus in field isolates of Leishmania peruviana.

Genetic diversity within and between tandemly arrayed copies of the Gp63 gene occurs in laboratory isolates of Leishmania spp., but the extent to which this represents natural genetic diversity has not been assessed. Here, the Gp63 locus is examined in 58 fresh isolates of L. peruviana, and clones derived from them, collected throughout the Peruvian Andes. Extensive polymorphism is observed, both in size of Gp63 containing chromosomes, and for restriction-fragment-length polymorphisms (RFLPs) at the Gp63 locus. All clones within an isolate are identical, including those with two distinct Gp63-hybridising chromosomal-sized pulsed-field gel electrophoresis (PFGE) bands, consistent with diploidy but with size differences in homologous chromosomes. For RFLP analysis, three enzymes were selected to cut within the coding region (PstI), in the intergenic region (SalI) and outside (EcoRI) the Gp63 gene cluster. PstI gave identical banding patterns across all isolates/clones. For EcoRI and SalI, all clones within an isolate were identical, but isolates were polymorphic for fragments at 13 (2-30 kb) and 8 (2.6-8.8 kb) different molecular mass locations generating 19 and 16 distinct RFLP patterns or genotypes for each enzyme, respectively. EcoRI restriction patterns, analysed by PFGE, were consistent with the presence of two clusters of Gp63 genes on each homologous chromosome, one contained within EcoRI fragments large enough to carry from 3 to 10 copies of the Gp63 gene, the second on fragments which could carry 1 or 2 copies of the gene. SalI patterns indicated variable restriction sites within clusters, but not within every intergenic region. A hierarchical analysis of variance of allele frequencies, expressed in terms of Wright's F-statistic, indicated significant barriers to gene flow at all levels, valleys within regions (north/south), villages within valleys, and individuals within villages. This extreme polymorphism at the Gp63 locus of L. peruviana demonstrates the great potential for generation of genetic diversity in parasite populations.

Animals↗
PubMed1994

Chair dental anaesthesia: a survey of current and intended practice in Mersey Region.

We performed a questionnaire survey to establish the current and intended practice of chair dental anaesthesia amongst recently appointed consultants, and senior registrars within the Mersey Region. Only 26% of the consultants surveyed practised chair dental anaesthesia, however, more consultants would have had their anaesthetic sessions allowed. Consultants performed 4.62 +/- 3.5 sessions per month and anaesthetised 8.0 +/- 2.27 patients per session. Cases were mostly performed in community dental centres. The most common reason for discontinuing the practice of chair dental anaesthesia was the Poswillo report and its implications. Sixty-eight per cent of senior registrars declared an interest in chair dental anaesthesia. This group had received significantly more training (p < 0.005) in dental anaesthesia than those with no interest. Most anaesthetists (52/71) felt that chair dental anaesthesia was acceptable in centres approved to Poswillo standards; 16 anaesthetists felt that it should be confined to a hospital environment and three felt that it should not be performed at all.

Ambulatory Surgical Procedures↗
PubMed1993

An evaluation of a short course in resuscitation training in a district general hospital.

One hundred nurses underwent a short form of cardiopulmonary resuscitation training, comprising a short lecture and practical training. This significantly improved theoretical knowledge, but failed to achieve a uniformly high standard of practical skill. After initial training the subjects were divided into three groups. Group 1 underwent monthly refresher sessions, Group 2 a single refresher at 3 months and Group 3 no refresher training prior to re-testing of all subjects at 6 months. Knowledge and skills had declined in all three groups. Theoretical scores were better preserved in the groups having refresher training. Frequent refresher training failed to improve the poor initial performance in the practical skills. The time required to produce adequate training is greater than that which can be provided by clinical staff and necessitates the appointment of a resuscitation training officer.

Cardiopulmonary Resuscitation↗
PubMed1993

Death certification.

Explore the source record for details and available documents.

Cardiopulmonary Resuscitation↗