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A Córdoba

Publications and source records attributed to A Córdoba.

33 records · Page 2Linked to original sources
PubMedDate not supplied

[Sentinel node in breast cancer. Histological study of 67 cases].

BACKGROUND: The extirpation of the sentinel node is a widespread surgical technique whose aim is to avoid axillary resection in patients with breast cancer at early stages. Determination of tumoral infiltration can be evident in the case of macrometastasis (>2mm), or difficult to detect in micrometastasis (<2mm). For this reason we use a protocol of serialized sections, to increase our capacity for detecting micrometastasis. Realisation of this protocol is highly laborious and represents a high cost, thus its effectiveness and efficacy must be studied. MATERIAL AND METHODS: We reviewed 67 sentinel nodes corresponding to 48 patients with breast cancer treated at our hospital in the last three years. All the samples received at the Pathology Unit were included. Serialized sections of three microns were made on each node and with a depth of 40 microns between them. A total of fourteen cuts were made. Every five cuts there was an alternation of immunostaining with a cocktail of queratines (AE1/AE3) and with haemotoxylin-eosin. RESULTS: Sixty-seven sentinel nodes were extirpated from 48 patients. Fourteen positive (20%) were detected. Of these metastases, 6 were macrometastases >2mm, 5 were micrometastases <2mm and 3 were isolated cellular groups (between 0.2 and 2mm). In 6 cases (43%), the metastases only became evident with queratine staining in serialized cuts. Axillary resection was carried out in 44 cases. CONCLUSIONS: The histological study of the sentinel nodes makes it possible to evaluate the status of tumoral infiltration of the axillary ganglions and to greatly reduce axillary lymphadenectomies. The conventional study of the sentinel node (with a single section) is insufficient for the diagnosis of tumoral infiltration. In our series, histological study using a protocol of serialized sections has enabled us to detect some 43% of metastases in the sentinel node that were not evident in the initial section.

Breast Neoplasms↗
PubMedDate not supplied

[Modulation of biomarkers expression (OR, PR and C-erbB2) in breast cancer following neoadjuvant treatment].

INTRODUCTION: Pre and post-operative oncological therapy in patients with breast cancer is determined, amongst other factors, by hormone receptor status and by c-erbB2 expression. The aim of this study is to determine the influence of neoadjuvant therapy on the expression of oestrogen receptor (OR), progesterone receptor (PR) and c-erbB2. METHODS: Fifty-three patients with breast cancer diagnosed by tru-cut biopsy were studied. Patients with locally advanced carcinoma (20) had preoperative treatment. All patients underwent surgical resection. Expression of OR, PR and c-erbB2 in both the tru-cut biopsy and the gross specimen was compared. RESULTS: We found significant differences in OR, PR expression in both biopsy and gross specimen, between the group of patients who underwent neoadjuvant treatment and the group without pre-surgical treatment. Changes in PR, OR and c-erbB2 status were found between the tru-cut biopsy and the gross specimen, in about 10 to 40% of the cases who received neoadjuvant therapy. These changes had no statistical significance.

Breast Neoplasms↗
PubMedDate not supplied

[Comparative results of ganglion dissection in cancer of the rectum with and without prior treatment of the adipose tissue].

BACKGROUND: The aim of this study is to describe the result obtained through two techniques of isolation of lymphatic lymph nodes in surgical pieces of anterior resection due to adenocarcinoma of the rectum. MATERIAL AND METHODS: We carried out a search in a series of 30 consecutive cases of patients operated on for adenocarcinoma of the rectum for lymph nodes first in a manual conventional way and second after 24 hours in a degreasing solution at room temperature. RESULTS: In the first search 335 lymph nodes were lymph nodes isolated, with an average that oscillated between 6.46 and 17.58, with the lower values corresponding to the groups that had received prior adjuvant treatment. In the second inclusion, following the action of the clearing solution, we found new lymph nodes (85) in some 75% of the cases, appreciably lower in number and smaller in size than the initial search. CONCLUSIONS: Manual lymph nodes dissection of the adipose tissue is a reliable method for the isolation of lymphatic lymph nodes in pieces of resection due to adenocarcinoma of the rectum. The search for lymphatic ganglions following the action of a clearing solution should be reserved for cases in which the minimum recommended in the TNM staging is not reached

Adenocarcinoma↗
PubMedDate not supplied

[Meningeal melanocytoma. Presentation of a case and review of the literature].

We would present a fifteen years old girl's case, which after two years from the beginning of his symptoms; a pigmented meningioma was removed at the cervical level of the spinal cord. It showed morphologically and clinically like the named by Limas "meningeal melanocytoma". Four years after the operation, the girl developed a malignant melanoma with invasion of the posterior mediastinum. We conclude that we ought to consider the meningeal melanocytoma as a borderline tumour, similar in the other way to the cellular blue naevus and the spindle A melanoma of the uveal tract.

Adolescent↗
PubMedDate not supplied

[Factors affecting the in vitro excystation of Cryptosporidium sp].

To assess the viability of the oocysts from Cryptosporidium sp, an in vitro excystation technique was developed and optimized. To this end, oocysts from Cryptosporidium sp were isolated from a sample of fecal material from a child with symptomatic cryptosporidiosis. The specimen was processed by a discontinuous-sucrose-gradient technique yielding a final suspension of purified and concentrated oocysts. The resulting oocysts were quantified by counting an aliquot in a Neubauer hemocytometer chamber and the remainder were preserved in phosphate-buffered saline at pH 7.2 and 4 degrees C. The two protocols tested through the use of 100-microliter aliquots containing 178,000 oocytes/mm3 were: I. Treatment at 37 degrees C for 24 h with 1% (v/v) bile plus 0.44% (w/v) sodium bicarbonate. Group A: pretreated with acidified trypsin (pH 2.75) at 37 degrees C for 1 h; Group B: not pretreated. II. Treatment at 37 degrees C for 24 h with 1% (v/v) bile at pH 6 and at pH 8 either with or without 0.44% (w/v) sodium bicarbonate in air or an atmosphere containing 10% (v/v) CO2. Group 1: bile, pH 6; Group 2: bile, pH 6 plus CO2; Group 3: bile, pH 6 plus bicarbonate; Group 4: bile, pH 8 plus bicarbonate; Group 5: bile, pH 6 plus bicarbonate plus CO2; Group 6: bile, pH 8 plus bicarbonate plus CO2; Group 7: bile, pH 8; and Group 8: bile, pH 8 plus CO2. We then assessed the degree of excystation in all the groups by counting the number of intact oocysts in the hemocytometer. For the first protocol, the oocyst count after excystation revealed an average of 28,000 oocysts/mm3 in Group A and 22,000 in Group B. These values indicate excystation frequencies of 84.3% and 87.6% for these two groups, respectively. The corresponding excystation percentages for groups 1-8 of protocol II were 49.5, 56.7, 83.2, 88.3, 88.3, 92.7, and 94.1, respectively. Preincubation with trypsin failed to augment oocyst excystation in Cryptosporidium in any of these experimental circumstances. The optimal conditions for oocyst excystation in these 24 h incubations were thus seen to be in the presence of 1% (v/v) bile at pH 8, and 37 degrees C under an atmosphere of 10% (v/v) CO2.

Animals↗