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Biomedical subjects

A Calligaro

Publications and source records attributed to A Calligaro.

97 records · Page 6Linked to original sources
PubMedDate not supplied

Clinical value of a peculiar nuclear fluorescence staining in systemic sclerosis.

Using unfixed rat liver sections as substrate for the detection of antinuclear antibodies, distinct nuclear staining was observed in the sera of patients with systemic sclerosis. Its reaction differed from previously described fluorescence patterns and was characterized by numerous fine speckles throughout the nucleus associated with one or more coarse lines. These lines were more evident in diluted sera, when fine speckled fluorescence faded. This picture "fine speckles with lines" (FLS) was defined. The FLS pattern was present in 35% of 91 patients with systemic sclerosis, while it was absent in 517 patients with rheumatic and non-rheumatic disorders and in 100 matched healthy subjects. Moreover, the FSL pattern was significantly associated with the diffuse subset of systemic sclerosis (50% of cases) and was related to the presence of anti-Scl-70 antibody. These results indicate that this fluorescent pattern of antinuclear antibodies may be considered a simple and very useful aid in the diagnosis and prognosis of systemic sclerosis.

Adult↗
PubMedDate not supplied

Morphological and functional characteristics of the crista terminalis in the rabbit right atrium.

The structural and electrophysiological characteristics of the crista terminalis (CT) in the right atrium of the rabbit have been analyzed. The myocardial cells of the CT show a longitudinal and parallel arrangement corresponding to the long axis of the CT and can be divided into two distinct groups, i.e.: (1) smaller cells, that resemble those of the atrial common myocardium and constitute a thin superficial (subendocardial) layer; (2) larger clear cells, that are organized in a homogeneous thick and deeper row and display an extended T-tubular system. The action potentials of the surface of the whole CT preparation were heterogeneous and could be grouped into three main types. Such heterogeneity was possibly related to electrotonic coupling between atrial cells. The action potentials recorded from the deep cells of the CT were homogeneous, displaying high amplitude, very high maximum rate of rise and relatively long duration. The above data supply a correlation between morphological and electrophysiological findings. They suggest that the deep layer of the CT could be responsible for the high conduction rate.

Action Potentials↗
PubMedDate not supplied

CG5/Dx human breast cancer cell line: characterization of a new doxorubicin-resistant variant.

By continuous exposure of CG5 human breast cancer cell line to increasing doxorubicin (Dx) concentrations, a multidrug-resistant (MDR) subline (CG5/Dx) was obtained. The resistant variant showed P-glycoprotein (P-gp) expression and a lower intracellular doxorubicin level than the parental cells. CG5/Dx cells were 19.4 fold more resistant to Dx than CG5 cells and showed a cross-resistance to some structurally related and unrelated compounds. Differences in kinetics, biological and ultrastructural features between the two cell lines were investigated. The CG5/Dx cells grew more slowly, produced higher CEA levels and showed a reduced progesterone receptor (PgR) content than the parental cells. Ultrastructural studies revealed differences involving, polyribosomes, rough endoplasmic reticulum, [mitochondria] and cytoskeleton.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMedDate not supplied

Localization of proliferating cell nuclear antigen-immunoreactivity in human dental pulp and gingiva.

The proliferating cell nuclear antigen (PCNA) is regarded as an operational marker of proliferating cells. We have used PC10 monoclonal antibody to PCNA to reveal proliferation sites in human dental pulp and gingiva. Intense PCNA-immunoreactivity was observed in the basal layer of the gingiva lining epithelium and within some cells of the underlying connective tissues, including some endothelial and perivascular cells. PCNA-reactive cells were scattered throughout the pulp tissue, but were particularly numerous in the peripheral part. Since PCNA is an endogenous cell cycle-related molecule, we propose that PCNA-antibodies may represent useful for studying cell kinetics in human oral tissues in normal as well as pathological situations, such as tumours, wound healing and inflammation.

Basement Membrane↗