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A Canfield

Publications and source records attributed to A Canfield.

3 recordsLinked to original sources

Service learning: integrating student learning and community service.

Health professions education is directly effected by changes in health care service delivery and financing systems. In the United States, as the health care industry increasingly shifts to a market economy, service delivery venues are moving away from acute care facilities and into community-based settings. Additionally, there is increased emphasis on primary prevention programs, often provided in public health settings. For health professions programs that traditionally provide clinical training in hospitals and long-term care facilities, there are unique challenges associated with identifying new venues in order to insure that students are exposed to a wide variety of patients with a range of chronic to acute disease conditions. One set of tools that has demonstrated usefulness during these kinds of transitions is service learning. This teaching methodology emphasizes increased partnership with clinical training sites, extensive orientation to patient populations and community resources, structured reflection and instilling the ethic of service in future health care providers. Although this article describes utilization of service learning in the context of current conditions in the United States, we hope that the principles presented here can be readily adapted in any setting.

Journal Article↗

Molecular structure of heparan sulphate synthesised by bovine aortic endothelial cells.

The distribution and structure of heparan sulphate (HS) synthesised by bovine aortic endothelial cells (BAEC) has been studied. Confluent cultures were harvested and analysed as three separate compartments: (a) the culture medium, (b) the detergent-soluble cell-associated material and (c) the detergent-insoluble matrix material extracted with 6 M urea. HS was present in all three of the culture compartments, but the molecular size of the HS proteoglycans (PG) and the free polysaccharide chains varied according to compartment origin. The matrix pool accounted for almost 50% of the total HS which was present as a large HSPG possessing polysaccharide chains of 79 kDa. When studied in more detail, these large HS chains displayed an N-sulphate content and distribution (determined by low pH nitrous acid treatment) similar to that seen in the majority of other mammalian heparan sulphates. Extended iduronate sequences were also identified (i.e., heparitinase-resistant sequences); however, apart from these regions, the degree of O-sulphation was relatively low. In addition, the presence of heparin-like sequences (GlcNSO3(+/- 6S)-IdoA(2S)), characterised by heparinase sensitivity, accounted for only 5% of the disaccharides and such sequences appeared to be located with an ordered distribution, mainly in relatively short sulphated domains within the intact molecule. Given the strategic location of the large matrix-associated HSPG within the BAEC system studied, it is conceivable that the HS structure may be important in a number of functions such as cell attachment processes and/or the binding of growth factors.

Animals↗