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A Canut Blasco

Publications and source records attributed to A Canut Blasco.

At least 19 recordsLinked to original sources

[Familial outbreaks of fascioliasis: usefulness of serologic investigation by enzyme immunoassay].

BACKGROUND: To assess the usefulness of an enzyme immuno-assay test for the diagnosis of two familial outbreaks of Fasciola hepatica parasitosis in the Zamora area, where watercress are a normal part of the diet. PATIENTS AND METHODS: The microbiological diagnosis of two familial outbreaks of fascioliasis was analyzed, which included the search for eggs in feces by the Kato technique and two serologic tests, one screening test by indirect hemagglutination, and a confirmatory test by enzyme-immunoassay in 12 patients. RESULTS: Five out of the six seropositive patients had eggs detected in their feces. To note that two of the patients with eosinophilia that excreted eggs--one asymptomatic and the other with abdominal pain--had a positive result in the EIA test only and with high titers. CONCLUSIONS: The EIA test is useful for the diagnosis and study of fascioliasis outbreaks to interpret the significance of low titers in the screening test in patients with eosinophilia.

Adolescent↗

[Epidemiology and diagnosis of Cryptosporidium spp. parasitosis in children: usefulness of the serologic study].

BACKGROUND: Evaluate the contribution of the serological study of Cryptosporidium spp. for more precise knowledge of the epidemiology of this parasitation within a child population and its usefulness in laboratory diagnosis of cryptosporidiasis. METHODS: Using a prospective study design, the presence of antibodies for Cryptosporidium spp. was determined by indirect immunofluorescence in sera of 106 children, aged 0-13 years in the province of Salamanca. RESULTS: The seroprevalence of IgG against Cryptosporidium spp. n the child population was 22.6% (24/106). 20.8% of the children seropositive for IgG (5/24) also presented with specific IgM. In a newborn, IgG (12.5%; 1 out of 8 newborns) was detected but IgM not found. The seroprevalence of IgG in rural areas was 18.2% (10/55) and the average in urban areas was 27.4% (14/51). In both instances, the age group with the highest seroprevalence corresponded to that of 2-3 year olds. CONCLUSIONS: The rate of seroprevalence of Cryptosporidium spp. in the child population of Salamanca is appreciably higher than the prevalence detected in feces. Recent infection can manifest itself with simultaneous seropositivity of IgG and IgM. This serological pattern, in the presence of a clinical framework compatible with the detection of oocysts in feces, can complement the diagnosis of cryptosporidiasis.

Adolescent↗

[The detection of Cryptosporidium spp. in the feces of a preschool population: a comparison of 5 staining methods].

The usefulness of five dying techniques to detect oocysts of Cryptosporidium spp in stools is evaluated in a healthy children preschool population, taking from a smear of the sediment obtained in the formalin-ether concentration technique (Ritchie's test). 10 positive stool samples were detected of a total of 115 samples (8.7%). Dying with Lugol the sediment of Ritchie's test only detected 40% of positive samples. Modified Ziehl-Neelsen dye, auramine dye and indirect immunofluorescence dye with monoclonal antibodies, yielded an excellent diagnostic correlation (each of them detected 90% of the positive samples). Indirect immunofluorescence dye with specific human antibodies detected one case more of parasites than the rest of dyes, is not expensive and easy to perform.

Animals↗

[Focal infections caused by non-typhi Salmonella: a review of our case series and comparison with other series].

Nine cases of local infection due to non typhi Salmonella enterica, some of them of unusual localization, in 8 patients (mean age 64.9 +/- 12.4 years) attended in Zamora's Virgen de la Concha Hospital over a period of five years, are described. Focal salmonellosis represented 1.5% of non-typhi salmonellosis cases in that period (9 out of 606 detected cases). 6 of the 8 patients (75%) showed a predisposing disease. In two patients the previous existence of gastroenteritis due to Salmonella was assessed and only in one of them concomitant bacteremia was detected. Soft-tissue infections were the more frequent clinical feature: plantar abscess, two abdominal wall abscesses--one of them after cholecystectomy--post-pericardiotomy thoracic wall abscess and perianal abscess. Three soft-tissue infections were due to group B serotypes. 4 out of five soft-tissue infections evolved favorably with surgical treatment. The rest of the series is formed by two cases with acute cholecystitis in patients with previous cholelithiasis (one of whom relapsed originating an abdominal wall abscess), a recurrent pleural empyema and a purulent pericarditis. The pericarditis was produced by S. enteritidis. Patient showed signs of cardiac tamponade, his condition improving after pericardial drainage and parenteral and intrapericardial administration of ciprofloxacin. Epidemiologic and clinic characteristic of our series are compared with other series of focal salmonellosis.

Abdominal Muscles↗

The prevalence of Cryptosporidium species in children in day care centres and primary schools in Salamanca (Spain): an epidemiological study.

Cryptosporidium species was found in 1.8% (2/113 children in the first sampling) and 1.9% (2/107 children in the second sampling) of the children studied in 11 day care centres. All cases were observed in children younger than 3 years of age, who were immunocompetent and asymptomatic. No cases of parasitization by Cryptosporidium species was detected in 67 children aged 5-14 years attending 7 primary schools.

Adolescent↗

[Incidence of Cryptosporidium sp in patients treated in a general hospital. Technics for the identification of oocysts in feces].

In feces referred for parasite investigation from 1973 patients of the Hospital Clinico Universitario in Salamanca, 27 instances of infestation by Cryptosporidium sp (1.5% of all investigated patients) were detected in 19 children (1.4%) and 8 adults (2.2%). The incidence in patients with antibodies against the human immunodeficiency virus (HIV) was 12.5%, in contrast with 1.25% in patients without anti-HIV antibodies. 55.5% of the instances of Cryptosporidium sp infestation were found in children less than 4 years old. The higher incidence was in winter and spring. The association with other enteropathogens was found in 14.8%. Diarrhea and abdominal pain were the most common clinical features of cryptosporidial disease. Chronic diarrhea was found in two adult patients with acquired immunodeficiency syndrome. In immunocompromised children and adults asymptomatic carriers were found. The sensitivity and specificity of immunofluorescence testing with monoclonal antibodies as compared with Ziehl-Neelsen stain for the detection of Cryptosporidium sp oocysts were 100%.

Acquired Immunodeficiency Syndrome↗

[Post-transfusion graft versus host disease in a patient with Hodgkin's disease].

A female with Hodgkin's disease developed graft versus host disease (GVHD) after the administration of two units of packed red cells. Ten days after the transfusion she developed fever and rash, with subsequent hepatic and intestinal disease and a profound bone marrow aplasia. She died from a Candida tropicalis sepsis. The diagnosis of GVHD was made on the basis of clinical and histological criteria. We review this uncommon complication of hemotherapy, with special emphasis on its differential clinical features and its prevention.

Adult↗

The incidence of cryptosporidiosis in children: a one-year prospective survey in a general hospital in Spain.

The importance of Cryptosporidium species as a cause of diarrhea in the childhood population has increased since the examination of fecal samples has been introduced in microbiological laboratories. A one-year prospective survey carried out on 699 children from rural and urban districts examined at the University Hospital in Salamanca (Spain) revealed 8 cases of cryptosporidiosis (1.1% of the children studied). Most of the cases (5 patients) were observed in the January-April period. Seven of the eight cases occurred in children younger than 3 years of age. All the children with cryptosporidiosis were immunocompetent. Diarrhea (75.0%) and vomiting (50.0%) were the most frequent clinical features of the cryptosporidiosis. In one quarter of the cases, other pathogens were associated. A comparison is made with the incidence of giardiasis, which is the commonest intestinal parasitosis in our community.

Child↗