Biomedical subjects

A Cebers

Publications and source records attributed to A Cebers.

10 recordsLinked to original sources

PubMed2003

Deformation of intracellular endosomes under a magnetic field.

We present a non-invasive method to monitor the membrane tension of intracellular organelles using a magnetic field as an external control parameter. By exploiting the spontaneous endocytosis of anionic colloidal ferromagnetic nanoparticles, we obtain endosomes possessing a superparamagnetic lumen in eukaryotic cells. Initially flaccid, the endosomal membrane undulates because of thermal fluctuations, restricted in zero field by the resting tension and the curvature energy of the membrane. When submitted to a uniform magnetic field, the magnetized endosomes elongate along the field, resulting in the flattening of the entropic membrane undulations. The quantification of the endosome deformation for different magnetic fields allows in situ measurement of the resting tension and the bending stiffness of the membrane enclosing the intracellular organelle.

Cell Membrane↗

PubMed2002

Anisotropy of the structure factor of magnetic fluids under a field probed by small-angle neutron scattering.

Small-angle neutron scattering is used to measure the two-dimensional diffraction pattern of a monophasic magnetic colloid, under an applied magnetic field. This dipolar system presents in zero field a fluidlike structure. It is well characterized by an interaction parameter K(0)(T) proportional to the second virial coefficient, which is here positive, expressing a repulsion of characteristic length kappa-10. Under the field a strong anisotropy is observed at the lowest q vectors. The length kappa-10 remains isotropic, but the interaction parameter K(T) becomes anisotropic due to the long-range dipolar interaction. However, the system remains stable, the interaction being repulsive in all directions. Thus we do not observe any chaining of the nanoparticles under magnetic field. On the contrary, the revealed structure of our anisotropic colloid is a lowering of the concentration fluctuations along the field while the fluidlike structure, observed without field, is roughly preserved perpendicularly to the field. It expresses a strong anisotropy of the Brownian motion of the nanoparticles in the solution under applied field.

Journal Article↗

PubMed2000

Instabilities of concentration stripe patterns in ferrocolloids

Equations describing the kinetics of the phase separation in ferrocolloids in a Hele-Shaw cell under the action of a rotating magnetic field are proposed. Numerical simulation on the basis of a pseudospectral technique demonstrates that upon the action of a rotating field on a magnetic colloid which undergoes the phase separation a periodical system of stripes parallel to the plane of a rotating magnetic field stripes is created. The period of a structure found numerically satisfactorily corresponds to the one calculated on the basis of the energy minimum. Thus, the undulation instability leading to the formation of chevron structures takes place if the tangential component of a rotating magnetic field is eliminated, whereas the normal component is increased at the same time. If during the development of the undulation deformations of a concentration pattern the magnetic Bond number is large enough the secondary instabilities may occur leading to the fingering of stripes to bring about merging and break-up of stripes. It is shown that an increase in the magnetic Bond number leads to the onset of the instability at the boundaries between the regions with homogeneous orientation of stripes as well as to formation of the characteristic hairpin patterns.

Journal Article↗

PubMed2000

Shape transitions of giant liposomes induced by an anisotropic spontaneous curvature.

We explore how a magnetic field breaks the symmetry of an initially spherical giant liposome filled with a magnetic colloid. The condition of rotational symmetry along the field axis leads either to a prolate or to an oblate ellipsoid. We demonstrate that an electrostatic interaction between the nanoparticles and the membrane triggers the shape transition.

Anisotropy↗

PubMed1996