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Biomedical subjects

A Chadli

Publications and source records attributed to A Chadli.

At least 37 records · Page 2Linked to original sources
PubMed1993

Study of lectin-ganglioside interactions by high-performance liquid affinity chromatography.

A high-performance affinity column containing immobilized modified GM1 (lyso-GM1) was used to study the binding of an endogenous human brain lectin (HBL) in comparison with other carbohydrate-binding proteins. The proteins are previously converted into biotinylated derivatives. Detection of biotinylated proteins in the eluates by a microtitre plate assay ensures good sensitivity. The maximum binding capacity of the adsorbent for HBL is obtained in Tris buffer supplemented with beta-mercaptoethanol. The binding is inhibitable by specific sugar. It is concluded that the use of immobilized glycolipids in analytical high-performance liquid affinity chromatographic methods may serve as models in the study of interactions between gangliosides and carbohydrate-binding proteins.

Biotin↗
PubMed1992

Development of screening methods for detection of carbohydrate-binding proteins by use of soluble glycosylated polyacrylamide-based copolymers.

Mammalian endogenous carbohydrate-binding proteins (lectins) play fundamental roles in a variety of mechanisms of interactions both at the molecular and cellular levels. We have investigated the binding of one of them (human brain lectin) to soluble acrylamide copolymerized with derivatives of either lactose (O-beta-lactosyloxyallylallylaminoacrylamide copolymer) or D-mannose (D-alpha-mannosyloxyallylallylaminoacrylamide copolymer) in direct enzyme affinoassays, in an attempt to develop simple procedures for detection and estimation of its carbohydrate-binding activity. Biotinylated plant lectins were utilized as reference standards. Affinoassays employed the polymer dotted on nitrocellulose and the polymer coated on microtiter plates as well as detection of bound biotinylated lectin by streptavidin/horseradish peroxidase reagent. Both assays provided reproducible binding, inhibitable by specific sugars. The microtiter plate assay is well suited to sensitive detection of the negative endogenous lectin by competition with biotinylated brain lectin. We conclude that the use of derivatized acrylamide in dotting and microtiter plate assays may prove practical for detection of endogenous lectins and that such polymers may serve as model substances in the study of biological partners of these carbohydrate-binding proteins.

Acrylic Resins↗
PubMed1988

[Malignant lymphoma on a gastric stump].

We report a case of lymphoplasmocytic and immunoblastic gastric remnant lymphoma occurring in a 51 year-old male. This patient had undergone subtotal gastrectomy for a gastric peptic ulcer at age 37. Histologic findings in the initial gastric specimen showed a dense lymphoid infiltrate surrounding the ulcer within the gastric mucosa. This proliferation was grouped into distinct follicles, and was compatible with pseudo-lymphoma. The progression of pseudo-lymphoma to malignant lymphoma is suggested.

Adult↗
PubMed1987

[Histologic aspects of pleural mesothelioma].

The authors present four cases of malignant mesothelioma of pleura. Mean of age of our patients is 34 years. Two of them are men, the two others are women. The histologic study shows that our cases are represented by one of epithelial type, one of sarcomatoïd type and the two last of mixed biphasic type. The authors insist, in the basis of literature data, in the importance of the positive and differential diagnosis concerning the primitive and secondary epithelial tumors of the lung. The means of diagnosis are represented currently by cytology and histopathology and also by cytochemistry and immunohistochemistry.

Adenocarcinoma↗
PubMed1987

[Endosalpingosis: apropos of a case of colonic localization].

The endosalpingosis is a benign affection which is in the most often asymptomatic. It means the presence of tubal mucosa in ectopic location; often in the tubal wall; the pelvic peritoneal and adjacent lymph node. We present here a colic endosalpingosis case with intestinal occlusion. We review in this purpose the literature, and discuss the etiopathogenicity. It shows that a previously tubal affection can have a great part in the birth of this pathology.

Colonic Diseases↗
PubMed1987

[Fibro-inflammatory polyposis of the stomach: apropos of a case].

The stomach fibro-inflammatory-polyp is distinguished from the eosinophilic granuloma by some authors because of their geographic assessments. It is an inflammatory affection represented by a pseudo tumoral lesion. We present a case concerning a woman, 62 years old, who presented since 10 years a Biermer anaemia; a neoplastic affection was suspected, and then denied by histopathologic exam. We shall discuss the pathogenic and therapeutic data proposed by the literature. Recently, the treatment of this benign lesion consists in endoscopic resection. There no recurrence or malignant degeneration.

Anemia↗
PubMed1987

[Apropos of a case of lesional pulmonary edema advancing to fibrosis with cartilage metaplasia].

The non-hemodynamic pulmonary oedema by increase alveolo-capillary permeability or Adult Respiratory distress Syndrome (ARDS), has many causes and determine an acute insufficiency respiratory who needed mechanic ventilation. It can above all progressing to interstitial fibrosis after deadly. The writer present lesional pulmonary oedema case which evolved to fibrosis in a 17 old patient. This patient has been operated for duodenal ulcer with bi-vagotomy and antrectomy and presented a post operatory shock. The particularity of this observation is The fibrosis evolution to an cartilaginous metaplasia. The lesional pulmonary oedema has usually serious prognosis because the mortality is from 50 to 80% in the literature review.

Adolescent↗
PubMed1986

[Charles Nicolle and the accomplishments of his scientific thought].

The author presents in this study the achievements of the scientific thought of Charles Nicolle, chiefly on the basis of two notions: the asymptomatic diseases and the destiny of infectious diseases. Recalling the factors which prevailing about the career of Charles Nicolle, he shows that the asymptomatic diseases became a fundamental notion in infectious pathology as human, animal, viral, bacterial and parasitic diseases. The notion allowed him to open the study of pathogen agents on the fields of the environment, so enriching the epidemiology, the prophylaxis and the medical ecology. After the notion of specificity of microbes pointed out by Pasteur, Charles Nicolle enlarged their implications and conceived the infinite complexity of microbes. The first he introduced in microbiology the notion of mutation and foresaw the birth, the evolution and the death of the infectious diseases. Not so long time after and now these anticipations has been proved.

Communicable Diseases↗
PubMed1986

[Campaigns for the eradication of malaria in Tunisia: history and current situation].

The authors recall the historic of the different campaigns conducted against the malaria in Tunisia. Started since 1903 under the direction of Charles Nicolle, these campaigns were taken over after the end of each world war. However it must be necessary to wait the last campaign from 1966 to 1972, to conclude at the eradication of the disease, in 1977, into the country. Since this date, the systematic control of malaria is always conducted in the country. This end control is objected to avoid the resurgence of this parasitic disease in Tunisia, for the number of imported cases is increasing and the country presents ecological conditions propitious to Anopheles.

Animals↗
PubMed1986

[Malignant tumors of the stomach: apropos of 583 anatomo-clinical cases].

A retrospective study interesting 582 anatomoclinical cases of gastric malignant tumors was realised in two anatomo-pathological services of Tunis from 1970 to 1985. The average age of patients is 55 years old, with extensive ranges from 17 to 85 years old. The authors point out significative male predominance 64.6% of cases. The ulcered form is the most frequent, about 3.8% of macroscopic aspect cases. Among these last, the authors find 26.7% of ulcerating and polypoid form with invasive characteristics, and 18.83% of linitis plastica. The pyloric area localisation is the most frequent, 68% of cases. Diffuse tumors are observed in 8.47% of cases and numerous stomach localisation in 0.82% of cases. 84% of histological aspect are represented by adenocarcinoma, the most frequent of them (35%) is well differentiated type, then the poorly differentiated (20%) and atypic form (19.75%). Frequency of lymphosarcoma reaches 14.7%, the first is the centroblastic form, then lymphoblastic and lymphoplasmocytic forms. Concerning evolution, gastric cancer is an infiltrative tumor: serosal surface is attempted in 69.5% of cases, and during the first chirurgical act half cases show lymph nodes metastasis.

Adenocarcinoma↗
PubMed1986

[2 cases of mycotic infection caused by Cryptococcus neoformans].

The authors present two cases of Cryptococcus neoformans disease in two young persons. Its appears, in the first case, a diffuse ganglionnary and cutaneous lesion with an evolutive meningo-encephalic localization, and in the second case it appears only a meningitic lesion. These two observations are characterized by the apparition of the infection in a immunodepression patient and by an unfavorable evolution in spite of the treatment.

Adolescent↗