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Biomedical subjects

A Chamson

Publications and source records attributed to A Chamson.

49 records · Page 3Linked to original sources
PubMed1983

Evolutionary changes in acute-phase proteins in alcoholic hepatocellular diseases.

We studied the pattern of acute-phase proteins (orosomucoid, C-reactive protein, and haptoglobin) in hepatocellular deficiency due to chronic alcohol consumption, characterized by a decrease in serum transferrin concentration. We found that their patterns could vary independently of hepatocellular deficiency, but depend on the progression of hepatic disease. The most useful protein for discriminating the stage of inflammatory reaction is orosomucoid. In moderate hepatocellular deficiency, acute-phase proteins are increased independently of the decrease in transferrin, whereas in severe hepatocellular deficiency the acute-phase proteins are also decreased. Thus, it is possible to distinguish the two stages of hepatocellular deficiency by following changes in the concentration of orosomucoid.

Acute Disease↗
PubMed1980

[Under-hydroxylation of collagen synthesized by cultured fibroblasts].

The collagen secreted by dermal fibroblast cell cultures, previously labelled with 3H-proline, was extracted and purified by carboxymethyl cellulose. The ratio of hydroxyproline to proline showed that the collagen produced by cell cultures may be under-hydroxylated in comparison to the ratio observed in the types of collagen extracted from human skin. Then, it is concluded that it is not sufficient to determine only the hydroxyproline content for a collagen biosynthesis study in cell cultures. This low hydroxyproline content may explain the rapid catabolism of a part os newly synthetized collagen in vitro.

Cells, Cultured↗
PubMed1980

[Cytopharmacology in fibroblast culture].

Fibroblast cultures represent a particularly valuable model for the study of toxic or pharmacological effects upon collagen metabolism, the clinical expression of disturbances of which, corresponds to the process of fibrosis. The suggested method consists of the following: estimation of toxic concentrations by observation of the inhibition of cell growth and modifications of ultrastructure; a metabolic study corresponding to the measurement of biosynthesis and of the secretion of collagen and non-collagen proteins and determination of the type of collagen synthesized. The model was evaluated experimentally with tobacco smoke extracts and D-penicillamine.

Cells, Cultured↗
PubMed1979

Identification of a collagen-like antigen different from C1Q component of complement in serum.

The problem of the proportions of hydroxyproline proteins in serum linked to collagen and to the C1Q component of complement was examined. The C1Q component from human and bovine serum, and acid soluble calf skin collagen were purified. Antibodies to soluble collagen were prepared. With these antibodies we found by immunoelectrophoresis that in serum two distinct antigens exist: one reacts with antiserum to C1Q and the other reacts with antiserum to collagen. The collagen-like antigen may be precipitated by sodium chloride 4.3 mol/1.

Animals↗
PubMed1979

[Determination of free intracellular proline pool in fibroblast cultures].

By using a direct, rapid, sensitive colorimetric method, free intracellular proline pool from human adult skin fibroblasts in monolayer was determined. Thus under identical experimental conditions, free intracellular proline pool was about 20 pmol proline/micrograms cellular proteins, whatever the cell lines from human adult skin fibroblasts, the passage and the time of subculture. Besides, there was a poor correlation between collagen biosynthesis in fibroblast cultures and free intracellular proline pool.

Adult↗
PubMedDate not supplied

Effects of D-penicillamine on collagen biosynthesis by fibroblast cell cultures.

The effects of D-penicillamine on collagen and total protein synthesis by fibroblast cell cultures were studied. High concentration of D-penicillamine (10 mmol/l) produced a toxic effect consisting of inhibition of growth and a non-specific increase in protein synthesis. A low concentration of D-penicillamine (100 mumol/l) decreased the biosynthesis of both type I and type III collagens.

Cell Division↗
PubMedDate not supplied

Nutritional markers, acute phase reactants and tissue inhibitor of matrix metalloproteinase 1 in elderly patients with pressure sores.

BACKGROUND: Loss of collagen and elastin is observed in the elderly. In geriatric inpatients, chronic protein malnutrition could induce susceptibility to additional morbidity such as pressure sores. OBJECTIVE: The purpose of this study was to explore the relationship between nutritional and inflammatory status and the production of tissue inhibitor of matrix metalloproteinase 1 (TIMP-1). METHODS: Chronically ill elderly inpatients, without or with pressure sores, were enrolled. Nutritional protein markers, acute phase reactants, and TIMP-1 were determined, and changes in these biological parameters were compared. RESULTS: Chronic inflammatory process and protein malnutrition were observed in all enrolled patients. The severity of these two pathophysiological processes was independent of the occurrence of pressure sores. The serum prealbumin and albumin levels were lower in patients with pressure sores than in those without. In addition, the general increase in the TIMP-1 level was independent of the occurrence of pressure sores. The TIMP-1 level was mainly related to the prognostic inflammatory and nutritional index. CONCLUSIONS: The general increase in acute-phase reactants observed in the elderly could be related to the intercurrent diseases. The generally low serum albumin level, lowest in patients with pressure sores, may be considered evidence of protein malnutrition and hypercatabolism. Regarding the morbidity, the increase in TIMP-1 levels could be explained as an adaptive process to prevent intrinsic protein expenditure of extracellular matrices.

Acute-Phase Proteins↗
PubMedDate not supplied

[Unexpected occurence of hemoglobin Athens-Georgia].

The following report concerned a 47 year old Caucasian diabetic patient. Routine HPLC of HbA1c (Variant II Biorad Laboratories - hemoglobin A1c program) resulted only in the evidence of HbF (1%) and increase in HbA1c (10%). Considering the presence of HbF a standard agarose gel electrophoresis of patient's hemoglobin was performed and revealed the presence of Hb Athens-Georgia. Consequently the occurrence of HbF during determination of HbA1c by HPLC should lead to perform a standard hemoglobin electrophoresis in order to explore an hidden, unsuspected and clinically silent occurrence of rare Hb variant or additional unsuspected increase in HbA2.

Hemoglobins, Abnormal↗
PubMedDate not supplied

[About two cases of massive hypercholesterolemia].

Two patients presented massive hypercholesterolemia related to acquired disease, intrahepatic cholangitis with cholestasis in one and nephrotic syndrome in the second. Comparison of the lipoprotein patterns demonstrated distinctive pathophysiological processes different from those operating in primary causes of hypercholesterolemia.

Adult↗
PubMedDate not supplied

[Evolutionary comparison of laboratory cardiac and infectious parameters during septic shock: about an original case].

A massive release of troponin Ic and CKMB was described in a patient during septic shock. According to experimental animal models previously described, this release of biological markers by myocardial tissue could be due to an inflammatory process of myocardial tissue during septic shock without myocardial infarction in non cardiac critically ill patients.

Aged↗